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We narrowed to 2,492 results for: PUC19

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Showing: 2341 - 2360 of 2492 results

  1. pTCFL5-donor

    Plasmid
    #113808
    Purpose
    CRISPR donor plasmid to tag human transcription factor TCFL5 with GFP
    Depositor
    Kevin White
    Insert
    TCFL5 homology arms flanking EGFP-IRES-Neo cassette (TCFL5 Human)
    Use
    CRISPR
    Mutation
    hg38:20:62842215:T>A
    Available Since
    Dec. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    113808-plasmid-map-sequence-id-229886

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  2. pZNF250-donor

    Plasmid
    #112383
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF250 with GFP
    Depositor
    Kevin White
    Insert
    ZNF250 homology arms flanking EGFP-IRES-Neo cassette (ZNF250 Human)
    Use
    CRISPR
    Mutation
    hg38:8:144882271:C>G,hg38:8:144882273:T>C
    Available Since
    Dec. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    112383-plasmid-map-sequence-id-229838

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  3. pE2F5-donor

    Plasmid
    #112351
    Purpose
    CRISPR donor plasmid to tag human transcription factor E2F5 with GFP
    Depositor
    Kevin White
    Insert
    E2F5 homology arms flanking EGFP-IRES-Neo cassette (E2F5 Human)
    Use
    CRISPR
    Mutation
    hg38:8:85213859:T>C, hg38:8:85213860:T>G, h…
    Available Since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112351-plasmid-map-sequence-id-229665

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  4. pZNF23-donor

    Plasmid
    #112325
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF23 with GFP
    Depositor
    Kevin White
    Insert
    ZNF23 homology arms flanking EGFP-IRES-Neo cassette (ZNF23 Human)
    Use
    CRISPR
    Mutation
    rs1804984
    Available Since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    112325-plasmid-map-sequence-id-228545

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  5. pZNF764-donor

    Plasmid
    #112298
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF764 with GFP
    Depositor
    Kevin White
    Insert
    ZNF764 homology arms flanking EGFP-IRES-Neo cassette (ZNF764 Human)
    Use
    CRISPR
    Mutation
    hg38:16:30555233:G>A, hg38:16:30555234:C>G,…
    Available Since
    Nov. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    112298-plasmid-map-sequence-id-228408

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  11. H1_sgRNA(GRIN2B)_SauCas9_AcrIIA5-AsLOV2(N77)

    Plasmid
    #246056
    Purpose
    Expression of a sgRNA targeting the GRIN2B locus, a SauCas9 and an AcrIIA5-cpGR2 hybrid (insertion before N77) linked through a P2A sequence, under the same pol-III H1 promoter
    Depositor
    Dominik Niopek
    Article
    Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk
    Insert
    SauCas9, sgRNA(GRIN2B) and AcrIIA5_AsLOV2-N77
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Available Since
    Dec. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    246056-plasmid-map-sequence-id-495342

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  12. H1_sgRNA(GRIN2B)_SauCas9_AcrIIA5-AsLOV2(D41)

    Plasmid
    #246055
    Purpose
    Expression of a sgRNA targeting the GRIN2B locus, a SauCas9 and an AcrIIA5-cpGR2 hybrid (insertion before D41) linked through a P2A sequence, under the same pol-III H1 promoter
    Depositor
    Dominik Niopek
    Article
    Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk
    Insert
    SauCas9, sgRNA(GRIN2B) and AcrIIA5_AsLOV2-D41
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Available Since
    Dec. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    246055-plasmid-map-sequence-id-495341

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  13. qTAG-C-Solo-mScarlet-MAPRE1

    Plasmid
    #227323
    Purpose
    Donor template for mScarlet insertion into the C-terminus of the MAPRE1 locus. For growing microtubule tip visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MAPRE1 (Addgene #207793)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    MAPRE1 Homology Arms flanking a mScarlet Tag (MAPRE1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    227323-plasmid-map-sequence-id-451725

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  14. qTAG-C-Solo-mStayGold-ARL13B

    Plasmid
    #227277
    Purpose
    Donor template for mStayGold insertion into the C-terminus of the ARL13B locus. For cilia visualization. To be co-transfected with sgRNA plasmid pX330-PITCh-ARL13B (Addgene #227276)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    ARL13B Homology Arms flanking a mStayGold Tag (ARL13B Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227277-plasmid-map-sequence-id-452043

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  17. pDM097

    Plasmid
    #216819
    Purpose
    Aspergillus nidulans codon-adjusted mStayGold fluorescent protein, includes linker for N-terminal or internal tagging.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Insert
    mStayGold
    Tags
    FLAG-(SGGS)x2-XTEN16-(GGGGS)x3 and c4-(GGGGS)x2-X…
    Expression
    Bacterial
    Mutation
    Aspergillus nidulans codon-adjusted
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216819-plasmid-map-sequence-id-430144

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  18. pIRHDGAL3C

    Plasmid
    #218276
    Purpose
    Introduction of a LowTempGAL Turbo module (HD-GAL3C) to accelerate induction of GAL promoter induction
    Depositor
    Claudia Vickers
    Article
    Lu et al Nucleic Acids Res. 2024 May 29:gkae460.
    Insert
    fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pENO2> UBI4>RHGSGTMV>DHFRP66L>G*6>GAL3C(S509P)>tENO2-fcy1(454-778)
    Expression
    Yeast
    Mutation
    GAL3 (S509P), DHFR (P66L)
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218276-plasmid-map-sequence-id-433677

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  19. pINER3R

    Plasmid
    #185883
    Purpose
    In vivo gene amplification (HapAmp) of nerolidol synthetic module at RPL25 locus (15 copy).
    Depositor
    Claudia Vickers
    Article
    Peng et al Nat Commun. 2022 May 24;13(1):2895. doi:
    Insert
    PRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PGAL2>Y.FAST-EVBR1.2A-AcNES1>TRPL41B-PPDA1>RPL25(part;Arm2)
    Expression
    Yeast
    Mutation
    None
    Available Since
    April 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    185883-plasmid-map-sequence-id-430023

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Showing: 2341 - 2360 of 2492 results