We narrowed to 10,052 results for: cas9 expression plasmid

1. pUDP003

   Plasmid

   #101164

   Purpose

   E. coli/S. cerevisiae amdS shuttle vector allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)

   Depositor

   Jean-Marc Daran

   Article

   de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ScTDH3

   Available since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJW1328

   Plasmid

   #69488

   Purpose

   sgRNA(F+E) targeting pha-1 (no Cas9); for co-conversion in C. elegans

   Depositor

   Jordan Ward

   Insert

   sgRNA(F+E) targeting pha-1
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   R07E5.16 U6 promoter

   Available since

   Sept. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. TLCV2

   Plasmid

   #87360

   Purpose

   LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive sgRNA expression.

   Depositor

   Adam Karpf

   Article

   Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

   Has Service

   Cloning Grade DNA

   Insert

   Cas9-2A-eGFP
   

   Use

   CRISPR and Lentiviral; Doxycycline inducible; egf…

   Tags

   Cas9-T2A-eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Tight TRE promoter

   Available since

   Feb. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNOC-CRISPR-GFP

   Plasmid

   #100009

   Purpose

   Expresses Cas9-GFP and sgRNA in Nannochloropsis oceanica, contains hygromycin resistance cassette, for integration into geneome

   Depositor

   Eva Farre

   Article

   Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

   Type

   Empty backbone

   Use

   Algae, nannochloropsis expression

   Tags

   Cas9-GFP

   Expression

   Mutation

   Promoter

   Ribi promoter

   Available since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. MSP2283

   Plasmid

   #70702

   Purpose

   Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Inserts

   mammalian codon-optimized Staphylococcus aureus Cas9
   SaCas9 sgRNA (+84)
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSP2266

   Plasmid

   #70705

   Purpose

   Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Inserts

   mammalian codon-optimized Staphylococcus aureus Cas9
   SaCas9 sgRNA (+84)
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEasyG3_nat

   Plasmid

   #184918

   Purpose

   Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_nat recombines in vivo with a PCR product from pEasyG3_mic.

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEasyG3_mic

   Plasmid

   #184920

   Purpose

   Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_mic recombines in vivo with a PCR product from pEasyG3_zeo/nat/hph.

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEasyG3_hph

   Plasmid

   #184919

   Purpose

   Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_hph recombines in vivo with a PCR product from pEasyG3_mic.

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEasyG2_zeo

    Plasmid

    #184913

    Purpose

    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_zeo recombines in vivo with a PCR product from pEasyG2_mic.

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEasyG2_nat

    Plasmid

    #184914

    Purpose

    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_nat recombines in vivo with a PCR product from pEasyG2_mic.

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEasyG2_hph

    Plasmid

    #184915

    Purpose

    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_hph recombines in vivo with a PCR product from pEasyG2_mic.

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEasyG2_mic

    Plasmid

    #184916

    Purpose

    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_mic recombines in vivo with a PCR product from pEasyG2_zeo/nat/hph.

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEasyG3_zeo

    Plasmid

    #184917

    Purpose

    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_zeo recombines in vivo with a PCR product from pEasyG3_mic.

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV-T7-AcrIIA1-NLS(SV40) (KAC478)

    Plasmid

    #133795

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA1 with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

    Insert

    human codon optimized AcrIIA1
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CMV and T7

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. lentiCRISPR v2

    Plasmid

    #52961

    Purpose

    Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

    Inserts

    Cas9
    Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available since

    May 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-ABE8eWQ

    Plasmid

    #161815

    Purpose

    Expresses ABE8eWQ in mammalian cells

    Depositor

    Sangsu Bae

    Insert

    bpNLS-TadA8e(V106W/D108Q)-32AA linker-hSpCas9(D10A)-bpNLS-P2A-EGFP-NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Promoter

    Available since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. HCP4

    Plasmid

    #166106

    Purpose

    This plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the center of Cyr1 and the other targets a non-coding region on chromosome X (location X1)

    Depositor

    Matthias Heinemann

    Insert

    Cyr1Del-sgRNA/X1-sgRNA (CYR1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HCP6

    Plasmid

    #166108

    Purpose

    This plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the C-terminus of Cyr1 and the other targets a non-coding region on chromosome X (location X1)

    Depositor

    Matthias Heinemann

    Insert

    Cyr1CT-sgRNA/X1-sgRNA (CYR1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFOXA3.1.0-gDNA

    Plasmid

    #112417

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor FOXA3

    Depositor

    Kevin White

    Insert

    FOXA3 (FOXA3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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