We narrowed to 6,256 results for: tTA

Showing: 2341 - 2360 of 6256 results

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sg3

Plasmid

#113968

Purpose

Single short guide RNA targeting CAATGTATCTTATCATGTC

Depositor

Jordan Green

Article

Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

Insert

sg3

Expression

Mammalian

Promoter

U6

Available Since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

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sg2+sg3

Plasmid

#113970

Purpose

Double short guide RNA targeting TACCACATTTGTAGAGGTT & CAATGTATCTTATCATGTC

Depositor

Jordan Green

Article

Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

Insert

sg2+sg3

Expression

Mammalian

Promoter

U6

Available Since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

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mPLD2-PX (62-192)

Plasmid

#119128

Purpose

Bacterial expression of human phox homology (PX) domain, mPLD2-PX (62-192)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

mPLD2-PX (62-192)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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FLAG-MBP1-K295TAG-W340A-S233H-T237C-CAAX.pcDNA3-k

Plasmid

#127403

Purpose

Expresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site

Depositor

Sharona Gordon

Article

Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

Insert

Maltose Binding Protein

Tags

CAAX and FLAG

Expression

Mammalian

Mutation

Amino acid mutations: K295 to Amber codon, W340A,…

Promoter

CMV

Available Since

July 3, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-MBP1-E322TAG-K305H-E309H-W340A-CAAX.pcDNA3-k

Plasmid

#127407

Purpose

Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site

Depositor

Sharona Gordon

Article

Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

Insert

Maltose Binding Protein

Tags

CAAX and FLAG

Expression

Mammalian

Mutation

Amino acid mutations: E322 to Amber codon, K305H,…

Promoter

CMV

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-MBP1-K295TAG-W340A-S233H-T237H-CAAX.pcDNA3-k

Plasmid

#127401

Purpose

Expresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site

Depositor

Sharona Gordon

Article

Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

Insert

Maltose Binding Protein

Tags

CAAX and FLAG

Expression

Mammalian

Mutation

Amino acid mutations: K295 to Amber codon, W340A,…

Promoter

CMV

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pSiren_EGFP-shHmga2#2

Plasmid

#122290

Purpose

Knockdown for mouse Hmga2

Depositor

Yukiko Gotoh

Article

Kishi et al Nat Neurosci. 2012 Aug;15(8):1127-33. do

Insert

Knockdown shRNA for mouse Hmga2 (Hmga2 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-SUV39h2 ts3

Plasmid

#115885

Purpose

SUV39h2 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SUV39h2 shRNA (SUV39H2 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-Stx3-L77A/L80A

Plasmid

#100273

Purpose

Expresses GST-Stx3-L77A/L80A in bacteria.

Depositor

Thomas Weimbs

Article

Giovannone et al Sci Rep. 2020 Dec 7;10(1):21350. doi: 10

Insert

Stx3-1-265-L77A/L80A (Stx3 Rat)

Tags

GST

Expression

Bacterial

Mutation

No transmembrane domain; residues 4-264 present, …

Promoter

Tac

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-Stx3-E78A/E83A

Plasmid

#100272

Purpose

Expresses GST-Stx3-E78A/E83A in bacteria.

Depositor

Thomas Weimbs

Article

Giovannone et al Sci Rep. 2020 Dec 7;10(1):21350. doi: 10

Insert

Stx3-1-265-E78A/E83A (Stx3 Rat)

Tags

GST

Expression

Bacterial

Mutation

No transmembrane domain; residues 4-264 present, …

Promoter

Tac

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-Stx3-LE165/166AA

Plasmid

#100271

Purpose

Expresses GST-tagged open-conformation mutant of Stx3 in bacteria.

Depositor

Thomas Weimbs

Article

Giovannone et al Sci Rep. 2020 Dec 7;10(1):21350. doi: 10

Insert

Stx3-1-265-LE165/166AA (Stx3 Rat)

Tags

GST

Expression

Bacterial

Mutation

No transmembrane domain; residues 4-264 present, …

Promoter

tac

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-puro-eGFP-HEC1-d80

Plasmid

#114025

Purpose

Inducible expression of eGFP-tagged HEC1-d80 in FLPin Trex cell lines

Depositor

Geert Kops

Article

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Insert

eGFP-d80-HEC1 (NDC80) (NDC80 Human)

Expression

Mammalian

Mutation

Hec1 - 1 to 80 deleted

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-hygro-eGFP-HEC1-d80

Plasmid

#114030

Purpose

Inducible expression of eGFP-tagged HEC1-d80 in FLPin Trex cell lines

Depositor

Geert Kops

Article

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Insert

eGFP-d80-HEC1 (NDC80) (NDC80 Human)

Expression

Mammalian

Mutation

Hec1 - 1 to 80 deleted

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-hygro-eGFP-HEC1-8A/S15D

Plasmid

#114031

Purpose

Inducible expression of eGFP-tagged HEC1-8A/S15D in FLPin Trex cell lines

Depositor

Geert Kops

Article

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Insert

eGFP-HEC1-8A/S15D (NDC80) (NDC80 Human)

Expression

Mammalian

Mutation

Hec1 - 8A/S15D

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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RNAPII_CTD27-52_N2A

Plasmid

#98677

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II with N2A

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Tags

6xHis

Expression

Bacterial

Mutation

N1775A, N1782A

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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RNAPII_CTD27-52_del1961-1970

Plasmid

#98680

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II with del1961-1970

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Tags

6xHis

Expression

Bacterial

Mutation

del1961-1970

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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RNAPII_CTD27-52_N2A_S1966C

Plasmid

#98682

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II with N2A and S1966C

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Tags

6xHis

Expression

Bacterial

Mutation

N1775A, N1782A, S1966C

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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RNAPII_CTD27-52_K5S_S1966C

Plasmid

#98683

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II with K5S and S1966C

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Tags

6xHis

Expression

Bacterial

Mutation

K1838S, K1859S, K1866S, K1873S, K1887S, S1966C

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pmCherry_C1 I3

Plasmid

#61398

Purpose

Expresses mCherry-tagged Inhibitor-3 in mammalian cells

Depositor

Hemmo Meyer

Article

Eiteneuer et al EMBO J. 2014 Oct 8. pii: e201489054.

Insert

Inhibitor-3 (PPP1R11 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/TO-GFP-I3

Plasmid

#61401

Purpose

Expresses GFP-tagged Inhibitor-3 in mammalian cells

Depositor

Hemmo Meyer

Article

Eiteneuer et al EMBO J. 2014 Oct 8. pii: e201489054.

Insert

Inhibitor-3 (PPP1R11 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only