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Addgene
Tanja Mittag Lab: Mittag Lab Nov 2021 plasmids
Unpublished

Plasmids from Article

ID Plasmid Purpose
178658WT A1-LCD +NLSBacterial expression of N-terminally 6His tagged WT A1-LCD (LCD: low-complexity domain, aa 186-320)
178659A1-LCD-12F+12YBacterial expression of N-terminally 6His tagged A1-LCD with twelve Phe residues replaced with Tyr (LCD: low-complexity domain, aa 186-320)
178660A1-LCD+7F-7YBacterial expression of N-terminally 6His tagged A1-LCD with seven Tyr residues replaced with Phe (LCD: low-complexity domain, aa 186-320)
178661A1-LCD-9F+6YBacterial expression of N-terminally 6His tagged A1-LCD with nine Phe residues removed, six Tyr added (LCD: low-complexity domain, aa 186-320)
178662A1-LCD-8F+4YBacterial expression of N-terminally 6His tagged A1-LCD with eight Phe residues removed, four Tyr added (LCD: low-complexity domain, aa 186-320)
178663A1-LCD-9F+3YBacterial expression of N-terminally 6His tagged A1-LCD with nine Phe residues removed, three Tyr added (LCD: low-complexity domain, aa 186-320)
178664A1-LCD-10RBacterial expression of N-terminally 6His tagged A1-LCD with ten Arg residues removed (LCD: low-complexity domain, aa 186-320)
178665A1-LCD-6RBacterial expression of N-terminally 6His tagged A1-LCD with six Arg residues removed (LCD: low-complexity domain, aa 186-320)
178666A1-LCD+2RBacterial expression of N-terminally 6His tagged A1-LCD with two Arg residues added (LCD: low-complexity domain, aa 186-320)
178667A1-LCD+7RBacterial expression of N-terminally 6His tagged A1-LCD with seven Arg residues added (LCD: low-complexity domain, aa 186-320)
178668A1-LCD-2KBacterial expression of N-terminally 6His tagged A1-LCD with two Lys residues removed (LCD: low-complexity domain, aa 186-320)
178669A1-LCD-3R+3KBacterial expression of N-terminally 6His tagged A1-LCD with three Arg residues replaced with Lys (LCD: low-complexity domain, aa 186-320)
178670A1-LCD-6R+6KBacterial expression of N-terminally 6His tagged A1-LCD with six Arg residues replaced with Lys (LCD: low-complexity domain, aa 186-320)
178671A1-LCD-10R+10KBacterial expression of N-terminally 6His tagged A1-LCD with ten Arg residues replaced with Lys (LCD: low-complexity domain, aa 186-320)
178672A1-LCD-4DBacterial expression of N-terminally 6His tagged A1-LCD with four Asp residues removed (LCD: low-complexity domain, aa 186-320)
178673A1-LCD+4DBacterial expression of N-terminally 6His tagged A1-LCD with four Asp residues added (LCD: low-complexity domain, aa 186-320)
178674A1-LCD+8DBacterial expression of N-terminally 6His tagged A1-LCD with eight Asp residues added (LCD: low-complexity domain, aa 186-320)
178675A1-LCD+12DBacterial expression of N-terminally 6His tagged A1-LCD with twelve Asp residues added (LCD: low-complexity domain, aa 186-320)
178676A1-LCD+12EBacterial expression of N-terminally 6His tagged A1-LCD with twelve Glu residues added (LCD: low-complexity domain, aa 186-320)
178677A1-LCD+7R+10DBacterial expression of N-terminally 6His tagged A1-LCD with seven Arg residues added, ten Glu residues added (LCD: low-complexity domain, aa 186-320)
178678A1-LCD+7R+12DBacterial expression of N-terminally 6His tagged A1-LCD with seven Arg residues added, twelve Glu residues added (LCD: low-complexity domain, aa 186-320)
178679A1-LCD+7K+12DBacterial expression of N-terminally 6His tagged A1-LCD with seven Lys residues added, twelve Glu residues added (LCD: low-complexity domain, aa 186-320)
178680A1-LCD-2R-2K+3DBacterial expression of N-terminally 6His tagged A1-LCD with two Arg residues removed, two Lys removed, three Asp added (LCD: low-complexity domain, aa 186-320)
178681A1-LCD-4R-2K+5DBacterial expression of N-terminally 6His tagged A1-LCD with four Arg residues removed, two Lys removed, five Asp added (LCD: low-complexity domain, aa 186-320)
178682A1-LCD-10G+10SBacterial expression of N-terminally 6His tagged A1-LCD with ten Gly residues replaced with Ser (LCD: low-complexity domain, aa 186-320)
178683A1-LCD-20G+20SBacterial expression of N-terminally 6His tagged A1-LCD with twenty Gly residues replaced with Ser (LCD: low-complexity domain, aa 186-320)
178684A1-LCD-30G+30SBacterial expression of N-terminally 6His tagged A1-LCD with thirty Gly residues replaced with Ser (LCD: low-complexity domain, aa 186-320)
178685A1-LCD+23G-23SBacterial expression of N-terminally 6His tagged A1-LCD with twenty-three Ser residues replaced with Gly (LCD: low-complexity domain, aa 186-320)
178686A1-LCD-30G+30S+7F-7YBacterial expression of N-terminally 6His tagged A1-LCD with thirty Gly residues replaced with Ser, seven Tyr residues replaced with Phe (LCD: low-complexity domain, aa 186-320)
178687A1-LCD-30G+30S-12F+12YBacterial expression of N-terminally 6His tagged A1-LCD with thirty Gly residues replaced with Ser, twelve Phe residues replaced with Tyr (LCD: low-complexity domain, aa 186-320)
178688A1-LCD-20G+20S+7F-7YBacterial expression of N-terminally 6His tagged A1-LCD with twenty Gly residues replaced with Ser, seven Tyr residues replaced with Phe (LCD: low-complexity domain, aa 186-320)
178689A1-LCD-20G+20S-12F+12YBacterial expression of N-terminally 6His tagged A1-LCD with twenty Gly residues replaced with Ser, twelve Phe residues replaced with Tyr (LCD: low-complexity domain, aa 186-320)
178690A1-LCD+23G-23S+7F-7YBacterial expression of N-terminally 6His tagged A1-LCD with twenty-three Ser residues replaced with Gly, seven Tyr replaced with Phe (LCD: low-complexity domain, aa 186-320)
178691A1-LCD+23G-23S-12F+12YBacterial expression of N-terminally 6His tagged A1-LCD with twenty-three Ser residues replaced with Gly, twelve Phe residues replaced with Tyr (LCD: low-complexity domain, aa 186-320)
178692A1-LCD-14N+14QBacterial expression of N-terminally 6His tagged A1-LCD with fourteen Gln residues replaced with Asn (LCD: low-complexity domain, aa 186-320)
178693A1-LCD-23S+23TBacterial expression of N-terminally 6His tagged A1-LCD with twenty-three Thr reisudes replaced with Ser (LCD: low-complexity domain, aa 186-320)
178694A1-LCD-14N-4Q+18GBacterial expression of N-terminally 6His tagged A1-LCD with fourteen Gln residues removed, four Asn removed, replaced with eighteen Gly (LCD: low-complexity domain, aa 186-320)

Antibodies from Article