We narrowed to 2,612 results for: ubiquitin

1. pAAV-UBC-FLEX-XRI-FLAG

   Plasmid

   #178057

   Purpose

   Expression Recording Islands (XRIs) for recording cellular physiological histories along intracellular protein self-assembly. Contains XRI subunit with FLAG tag, under UBC promoter with a FLEX switch.

   Depositor

   Edward Boyden

   Article

   Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   XRI-FLAG
   

   Use

   AAV

   Tags

   FLAG-MBP

   Expression

   Mammalian

   Mutation

   Promoter

   Human ubiquitin C (UBC) promoter

   Available since

   Oct. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTol2CG2-ubi:QFGal4-SV40pA

   Plasmid

   #155119

   Purpose

   Tol2 vector for ubiquitous expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis marker

   Depositor

   Bret Pearson

   Article

   Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

   Insert

   ubi:QFGal4 (ubb Zebrafish, Budding Yeast, Neurospora crassa)
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   ubiquitin B

   Available since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX6P2-MDMX (23-111)

   Plasmid

   #62064

   Purpose

   Expression of human MDM4 (residues 23-111) in e. coli

   Depositor

   Gary Daughdrill

   Article

   Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

   Insert

   MDM4 (MDM4 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   contains residues 23-111

   Promoter

   tac

   Available since

   Feb. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

4. mCerulean3-Bcl-W-FUGW

   Plasmid

   #166751

   Purpose

   Express mCerulean3 fused to Bcl-2 family protein, Bcl-W, in a plasmid compatable for lentiviral infection. Used to make stable BMK-DKO cell lines.

   Depositor

   David Andrews

   Article

   Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

   Insert

   Bcl-W (Bcl2l2 Mouse)
   

   Use

   Lentiviral

   Tags

   mCerulean3

   Expression

   Mutation

   Promoter

   human ubiquitin C

   Available since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX6P2-MDMX (1-120)

   Plasmid

   #62065

   Purpose

   Expression of human MDM2 (1-120) in e. coli

   Depositor

   Gary Daughdrill

   Article

   Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

   Insert

   MDM4 (MDM4 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   contains residues 1-120

   Promoter

   tac

   Available since

   Feb. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA6-N-3XFLAG-Ubac2

   Plasmid

   #123597

   Depositor

   Bruce Beutler

   Article

   Choi et al Science. 2019 May 10;364(6440). pii: 364

   Insert

   ubiquitin associated domain containing 2 (Ubac2 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX-4T-3-TRC8 513-664

   Plasmid

   #185352

   Purpose

   RING domain-containing GST fusion protein for ubiquitination assays.

   Depositor

   Allan Weissman

   Article

   Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

   Insert

   TRC8 (RNF139 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa 513-664 only

   Promoter

   Available since

   Aug. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pZE21/UBP1/ClpS

   Plasmid

   #98566

   Purpose

   Expresses truncated codon-opt S. cerevisiae UBP1 and E. coli ClpS in an artificial operon

   Depositor

   George Church

   Article

   Kunjapur et al Proc Natl Acad Sci U S A. 2018 Jan 16;11

   Inserts

   Truncated ubiquitin cleavase, codon optimized
   ATP-dependent Clp protease adapter protein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   In a synthetic operon downstream of UBP1 and Remo…

   Promoter

   Same as UBP1 (operon) and Tet promoter (aTc induc…

   Available since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. NLS-HA-2xMCP-Ezh2

   Plasmid

   #126590

   Purpose

   Expresses MCP (MS2 Coat Protein) fusion to Ezh2 in mammalian cells, lentiviral backbone

   Depositor

   David Segal

   Article

   O'Geen et al Epigenetics Chromatin. 2019 May 3;12(1):

   Insert

   2XMCP-Ezh2 (Ezh2 Synthetic, Mouse)
   

   Use

   Lentiviral

   Tags

   NLS-HA

   Expression

   Mammalian

   Mutation

   Promoter

   human ubiquitin C promoter

   Available since

   May 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSS02:cyt-Peredox-mCherry

    Plasmid

    #161743

    Purpose

    cytosolic expression of fluorescent NADH/NAD+ biosensor Peredox-mCherry in plants

    Depositor

    Markus Schwarzländer

    Article

    Steinbeck et al Plant Cell. 2020 Aug 13. pii: tpc.20.002

    Insert

    cyt-Peredox-mCherry
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Ubiquitin 10

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA4/TO/GFPZNF598-9P-9A

    Plasmid

    #141192

    Purpose

    Expresses GFP-ZNF598 mutated in polyproline streches in mammalian cells, can be used to make inducible cell line

    Depositor

    Simon Bekker-Jensen

    Article

    Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

    Insert

    ZNF598 (ZNF598 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    all 3 proline repeats are mutated to Alanine (9xP…

    Promoter

    CMV

    Available since

    June 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA6-N-HA-Ubac2

    Plasmid

    #123598

    Depositor

    Bruce Beutler

    Article

    Choi et al Science. 2019 May 10;364(6440). pii: 364

    Insert

    ubiquitin associated domain containing 2 (Ubac2 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pInducer10-UBC9-sh1

    Plasmid

    #168986

    Purpose

    Expresses RFP cDNA and UBC9 shRNA 1 in mammalian cells

    Depositor

    Ji Luo

    Article

    Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    Ubiquitin Conjugating Enzyme 9 (UBE2I )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ubc promoter

    Available since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. LvPolyUb

    Plasmid

    #218982

    Purpose

    L.variegatus polyubiquitin-C promoter

    Depositor

    Amro Hamdoun

    Insert

    L.variegatus polyubiquitinC promoter
    

    Use

    Sea urchin

    Tags

    Expression

    Mutation

    Promoter

    L.variegatus polyubiquitinC promoter

    Available since

    Aug. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. Flag-HA-DUB3

    Plasmid

    #22593

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    DUB3 (USP17L9P Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Nov. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOPINK-TRABID (NZF1, aa 1-33)

    Plasmid

    #65437

    Purpose

    Expresses K29-/K33-specific TRABID NZF1 (aa 1-33) in E. coli

    Depositor

    David Komander

    Article

    Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

    Insert

    TRABID (ZRANB1 Human)
    

    Use

    Tags

    His6-GST

    Expression

    Bacterial

    Mutation

    only codes for amino acids 1-33 of TRABID, which …

    Promoter

    T7

    Available since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pubb-QFDBD-2x AD*-VP16*-EcR, α-cry:EGFP

    Plasmid

    #180475

    Purpose

    Inducible gene expression vector pubb-QFDBD-2x AD*-VP16*-EcR, α-cry:EGFP

    Depositor

    Hyunju Ro

    Article

    Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

    Insert

    Zebrafish ubb promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-UBC-S1-GCaMP6f

    Plasmid

    #160722

    Purpose

    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter GCaMP6f (calcium indicator), Xpress tag, and S1 protein scaffold.

    Depositor

    Edward Boyden

    Article

    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

    Insert

    S1-GCaMP6f
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Human ubiquitin C (UBC) promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYUU

    Plasmid

    #159751

    Purpose

    Ubiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by YFP fluoresce.

    Depositor

    Wolfgang Lukowitz

    Article

    Angulo et al bioRxiv

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLVX-TetOne-Puro-PSMD4

    Plasmid

    #202666

    Purpose

    Tet-ON 3rd generation expression vector with inducible expression of PSMD4 and a puromycin selection cassette

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    proteasome 26S subunit ubiquitin receptor, non-ATPase 4 (PSMD4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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