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pDB023k

Plasmid

#99219

Purpose

Complementary plasmid for aaRS PACE; amber codons in T7 RNAP

Depositor

David Liu

Article

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

Ppsp [SD8] T7RNAP(S12*,S203*,S527*)

Mutation

amber codons at positions 12, 203, and 527 of T7 …

Promoter

Ppsp

Available Since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

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CENP-B mCherry PP1 gamma

Plasmid

#45221

Depositor

Iain Cheeseman, Michael Lampson

Article

Liu et al J Cell Biol. 2010 Mar 22;188(6):809-20.

Insert

CENP-B mCherry PP1 gamma

Expression

Mammalian

Promoter

T7

Available Since

May 31, 2013

Availability

Academic Institutions and Nonprofits only

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pCR1055

Plasmid

#71823

Purpose

Express Streptococcus pyogenes nCas9 (H840A) nickase carrying two C-terminal SV40 NLS

Depositor

Jacob Corn

Article

Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

Insert

T7pr_6xHis-MBP-TEV-SPynCas9 (H840A)-2xNLS

Use

CRISPR

Available Since

Feb. 4, 2016

Availability

Academic Institutions and Nonprofits only

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Myc-PSFΔPQ

Plasmid

#35187

Depositor

Benjamin Blencowe

Article

Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

Insert

PSF (SFPQ Human)

Tags

Myc

Expression

Mammalian

Mutation

Deletion of residues 34-113

Promoter

CMV

Available Since

Oct. 2, 2012

Availability

Academic Institutions and Nonprofits only
pAMP-CY1_NF1 T1 R1276P (+minintr, KDR)

Plasmid

#119202

Purpose

transfer vector

Depositor

Helen Morrison

Article

Cui et al Hum Mutat. 2018 Nov 8. doi: 10.1002/humu

Insert

NF1 type 1 mini-gene (NF1 Human)

Use

Transfer vector

Mutation

R1276P

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pAMP-CY1_NF1 T2 R1276P (+minintr, KDR)

Plasmid

#119203

Purpose

transfer vector

Depositor

Helen Morrison

Article

Cui et al Hum Mutat. 2018 Nov 8. doi: 10.1002/humu

Insert

NF1 type 2 mini-gene (NF1 Human)

Use

Transfer vector

Mutation

R1276P

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pRSETB-ro1GFP

Plasmid

#82365

Purpose

Inducible expression of redox sensitive GFP in bacteria

Depositor

S. James Remington

Article

Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

Insert

ro1GFP

Tags

6xHIS

Expression

Bacterial

Mutation

C48S/Q80R/S147C/Q204C

Promoter

T7

Available Since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pCDF-KpAvs4

Plasmid

#188807

Purpose

Codon optimized KpAvs4 with C-terminal His tag

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

KpAvs4

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCDF-EcAvs2

Plasmid

#188804

Purpose

Codon optimized EcAvs2 with C-terminal His tag

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

EcAvs2

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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CAG::PSAMQ79G:GlyR-IRES-GFP

Plasmid

#32482

Purpose

Expresses PSAM-GlyR (Q79G) neuronal inhibitor, with IRES-EGFP marker

Depositor

Scott Sternson

Article

Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

Insert

PSAMQ79G-GlyR

Tags

IRES-EGFP

Expression

Mammalian

Promoter

CAG

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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