We narrowed to 27,864 results for: NTS

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pX62

Plasmid

#46854

Purpose

TOP Hupki-puro2A-G245S plasmid to introduce mutant p53 to PLF ESC or MEFs by IMCE

Depositor

Monica Hollstein

Article

Wei et al Cell Cycle. 2011 Apr 15;10(8):1261-70. E

Insert

attB-Puro-2A and Hupki p53 G245S (TP53 Mouse, Human)

Expression

Mammalian

Mutation

G245S

Available Since

Aug. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pDEST17-cDAXX

Plasmid

#169729

Purpose

Bacterial expression of N-terminally 6His tagged cDAXX

Depositor

Tanja Mittag

Article

Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

Insert

DAXX (DAXX Human)

Tags

6 His

Expression

Bacterial

Promoter

T7

Available Since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG-FLEX.SF-iGluSnFR.A184V.mRuby3

Plasmid

#106205

Purpose

Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.A184V

Use

AAV

Mutation

GltI: A184V + mRuby3 fusion

Promoter

CAG

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pX68

Plasmid

#46857

Purpose

OP Hupki-puro2A-A138V plasmid to indroduce mutant p53 to PLF ESC or MEFs by IMCE

Depositor

Monica Hollstein

Article

Wei et al Cell Cycle. 2011 Apr 15;10(8):1261-70. E

Insert

attB-Puro-2A and Hupki p53 A138V (TP53 Mouse, Human)

Expression

Mammalian

Mutation

p53 A138V

Available Since

Aug. 22, 2013

Availability

Academic Institutions and Nonprofits only

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MAC-C Sars-CoV-2 NSP12_P314L

Plasmid

#194813

Purpose

Sars-CoV-2 NSP12_P314L in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

NSP12_P314L (ORF1ab Sars-CoV-2)

Tags

MAC-C

Expression

Mammalian

Mutation

P314L

Available Since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-MTS-KD-c-Src-FLAG

Plasmid

#44653

Depositor

Yoshimi Homma

Article

Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

Insert

v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)

Tags

FLAG tag and mitochondria-targeting sequence (MTS…

Expression

Mammalian

Mutation

changed lysine 298 to methionine

Promoter

CMV promoter

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Granulin 4-no linker

Plasmid

#176925

Purpose

express Granulin 4 without linker 5 in mammalian cells

Depositor

Thomas Kukar

Article

Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

Insert

Granulin 4 (GRN Human)

Tags

Twin-Strep and FLAG tags encoded after Signal Pep…

Expression

Mammalian

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Δ18 B.1.351v3

Plasmid

#169464

Purpose

Encodes SARS-CoV-2 B.1.351v3 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

Insert

SARS-CoV-2 B.1.351v3 Spike truncated to remove 18 amino acids (S )

Expression

Mammalian

Available Since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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SPDK3889(TRV-RNA2-pPEBV-MCS-AtMet-tRNA)

Plasmid

#149277

Purpose

Modified Tobacco rattle virus RNA2 vector that could be used for expression of sgRNAs

Depositor

Savithramma Dinesh-Kumar

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

Modified TRV RNA2 with PEBV promoter and AtMet-tRNA mobile RNA sequence

Use

T-dna vector

Mutation

T2318C (DNA) in PEBV coat protein sequence

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_mGFP-FGFR3-K508M

Plasmid

#191753

Purpose

Expression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-K508M leads to inactive receptor (target mutation: c.1523A>T; silent mutation added on purpose: c.1515A>T)

Depositor

Irene Tiemann-Boege

Article

Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

Insert

mGFP-FGFR3IIIc

Tags

mGFP

Expression

Mammalian

Mutation

c.1523A>T (p.K508M) + silent mutation c.1515A&…

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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