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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,478 results for: nls

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Showing: 2361 - 2380 of 6478 results

  1. pRDA174_EF1a_EnAsCas12a_BFP

    Plasmid
    #196724
    Purpose
    Sortable and selectable EnAsCas12a.
    Depositor
    Bernhard Schmierer
    Insert
    enASCas12a-HA-T2A-BSD-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS - HA and SV40 NLS
    Mutation
    E174R, S542R, K548R
    Promoter
    EF1a
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196724-plasmid-map-sequence-id-388065

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  2. BII-Sh-BnTW

    Plasmid
    #133365
    Purpose
    Piggybac vector for shRNA-mediated knockdown, with co-expression with tdTomato-NLS
    Depositor
    Blair Madison
    Insert
    tdTomato-NLS
    Tags
    Flag-tagged tdTomato
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available Since
    Jan. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    133365-plasmid-map-sequence-id-268571

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  3. lenti.Cas9.BFP.Blast

    Plasmid
    #196714
    Purpose
    WT SpCas9 with BSD-TagBFP for selection and sorting
    Depositor
    Bernhard Schmierer
    Insert
    Cas9-T2A-BSD-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Promoter
    EF1a
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196714-plasmid-map-sequence-id-388043

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  4. pLV-EBFP2-nuc

    Plasmid
    #36085
    Depositor
    Pantelis Tsoulfas
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Replace EYFP of the vector pEYFP-Nuc from Clontec…
    Available Since
    June 1, 2012
    Availability
    Academic Institutions and Nonprofits only
    36085-plasmid-map-sequence-id-44618

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  5. mEmerald-Nucleus-7

    Plasmid
    #54206
    Purpose
    Localization: Nucleus, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    SV40 T-Antigen Nuclear Localization Signal (NLS)
    Tags
    mEmerald-Nucleus
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  6. CROPSeq-PP7-p65-HSF

    Plasmid
    #196719
    Purpose
    CROP-Seq library plasmid for CRISPR-A with SAM system (p65-HSF1 activator recruited to PP7 binding loops in the tracrRNA of the guide).
    Depositor
    Bernhard Schmierer
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196719-plasmid-map-sequence-id-388066

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  7. pMG005_mNeonGreen_P2A

    Plasmid
    #237215
    Purpose
    Fluorescent reporter codon optimized for Dictyostelid species
    Depositor
    Jordi van Gestel
    Insert
    mNeonGreen-P2A
    Use
    Cloning
    Tags
    3xFLAG-tag, HA-tag, SV40-NLS, and nuleoplasmin NL…
    Available Since
    May 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    237215-plasmid-map-sequence-id-473907

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  8. pMG008_mCherry_P2A

    Plasmid
    #237216
    Purpose
    Fluorescent reporter codon optimized for Dictyostelid species
    Depositor
    Jordi van Gestel
    Insert
    mCherry-P2A
    Use
    Cloning
    Tags
    3xFLAG-tag, HA-tag, SV40-NLS, and nuleoplasmin NL…
    Available Since
    May 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    237216-plasmid-map-sequence-id-473906

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  13. pNB695

    Plasmid
    #60144
    Purpose
    Disintegrator plasmid encoding MET17 promoter driven green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    GrLuc
    Tags
    ClpXP degron and Nuclear localization signal (NLS…
    Expression
    Yeast
    Promoter
    MET17pr
    Available Since
    Dec. 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    60144-plasmid-map-sequence-id-96226

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  14. pNB697

    Plasmid
    #60146
    Purpose
    Disintegrator plasmid encoding MET17 promoter driven red beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    RdLuc
    Tags
    ClpXP degron and Nuclear localization signal (NLS…
    Expression
    Yeast
    Promoter
    MET17pr
    Available Since
    Dec. 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    60146-plasmid-map-sequence-id-96253

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  15. pNB690

    Plasmid
    #60134
    Purpose
    Yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by MET17 promoter.
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    YeLuc
    Tags
    ClpXP degron and Nuclear localization signal (NLS…
    Expression
    Yeast
    Promoter
    MET17pr
    Available Since
    Oct. 31, 2014
    Availability
    Academic Institutions and Nonprofits only
    60134-plasmid-map-sequence-id-96201

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  16. pNB754

    Plasmid
    #60157
    Purpose
    Green beetle luciferase with nuclear localization signal (NLS) and adjustable degron tag (ssrA), driven by LYS9 promoter.
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    GrLuc
    Tags
    ClpXP degron and Nuclear localization signal (NLS…
    Expression
    Yeast
    Promoter
    LYS9pr
    Available Since
    Dec. 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    60157-plasmid-map-sequence-id-96349

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  19. pNB693

    Plasmid
    #60145
    Purpose
    Disintegrator plasmid encoding MET17 promoter driven yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    YeLuc
    Tags
    ClpXP degron and Nuclear localization signal (NLS…
    Expression
    Yeast
    Promoter
    MET17pr
    Available Since
    Dec. 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    60145-plasmid-map-sequence-id-96228

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  20. pNB689

    Plasmid
    #60133
    Purpose
    Green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by MET17 promoter.
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    GrLuc
    Tags
    ClpXP degron and Nuclear localization signal (NLS…
    Expression
    Yeast
    Promoter
    MET17pr
    Available Since
    Oct. 31, 2014
    Availability
    Academic Institutions and Nonprofits only
    60133-plasmid-map-sequence-id-96189

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Showing: 2361 - 2380 of 6478 results