We narrowed to 4,371 results for: 1-Oct;

1. ATP1A1_G6_T804N_v1_Dual_epegRNA_tevopreQ1

   Plasmid

   #187458

   Purpose

   Coselection for prime editing in human cells. Vector for tandem expression of ATP1A1 T804N-G6 epegRNA (tevopreQ1) with a user-specified epegRNA. pU6-tevopreq1-GG-acceptor-like plasmid

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G6 T804N epegRNA (tevopreQ1) (ATP1A1 Human)
   

   Use

   CRISPR; Prime editing

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tandem U6 promoters

   Available since

   July 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTox-EGFPsite1_NGG-PAM (BPK740)

   Plasmid

   #181746

   Purpose

   p11-lacY-wtx1 derivative toxic plasmid encoding an SpCas9 target site for EGFP site 1 with an NGG PAM

   Depositor

   Benjamin Kleinstiver

   Article

   Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

   Insert

   ccdB toxin gene and SpCas9 target site (EGFP site 1 NGG PAM)
   

   Use

   Selection plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBW2286_pCAG-PV1-FlpO-N396-L1-ABI-NLS-BGHpA

   Plasmid

   #108769

   Purpose

   Expresses N-terminus to aa396 of Flp recombinase fused to ABI CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   Flp recombinase fragment N-terminus to aa396
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   ABI,NLS

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRevTRE MKL1 S449A/T450A/S454A

   Plasmid

   #19847

   Depositor

   Ron Prywes

   Article

   Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

   Insert

   MKL1 S449A/T450A/S454A (MRTFA Human)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mutation

   changed Serine 449, Threonine 450 and Serine 454 …

   Promoter

   Available since

   Dec. 16, 2008

   Availability

   Academic Institutions and Nonprofits only

5. pProEx-HTa-SOD1 C57D/C146D

   Plasmid

   #191851

   Purpose

   express C57D/C146D mutant SOD1 in bacterial cells

   Depositor

   Nam-Chul Ha

   Article

   Baek et al Commun Biol. 2022 Oct 12;5(1):1085. doi:

   Insert

   SOD1 (SOD1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C57D, C146D

   Promoter

   Available since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCWXPGR-pTF-DVL2deltaDIX

   Plasmid

   #114276

   Purpose

   TET-inducible expression of DVL2 protein with a deletion of its DIX-domain

   Depositor

   Patrick Salmon

   Article

   Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

   Insert

   DVL2 (Dvl2 Mouse)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   deletion of DIX domain

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330-Flag-SpCas9-HF1 (without sgRNA)

   Plasmid

   #92102

   Purpose

   Expression plasmid for human codon-optimized high-fidelity SpCas9-HF1, px330-like backbone (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   3xFlag-NLS-Streptococcus pyogenes Cas9-HF1-NLS
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, Q926A

   Promoter

   Cbh

   Available since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSD36_pCAG-PV1-FKBP-L1-TP901-327C-BGHpA

   Plasmid

   #108814

   Purpose

   Expresses aa327 to C-terminus of TP901 integrase fused to FKBP

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   TP901 integrase fragment aa327 to C-terminus
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   Sept. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCSDest2-SpCas9-NLS-3XHA-NLS-Zif268

   Plasmid

   #69219

   Purpose

   Expresses wild type SpCas9 fused to Zif268 in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   Zif268 (Egr1 Mouse)
   

   Use

   CRISPR

   Tags

   NLS-3XHA-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCin4-3xFLAG-MKL1 S449A/T450A/S454A

    Plasmid

    #19845

    Depositor

    Ron Prywes

    Article

    Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

    Insert

    MKL1 S449A/T450A/S454A (MRTFA Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    changed Serine 449, Threonine 450 and Serine 454 …

    Promoter

    Available since

    Dec. 16, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pLL5.0 hCortKD-Flcortmut3YF EGFP

    Plasmid

    #187263

    Purpose

    Lentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YF mutant

    Depositor

    Alpha Yap

    Article

    Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

    Insert

    Cortactin (CTTN Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    3YF (Y412, Y470, Y486)

    Promoter

    U6, 5'LTR

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBW2660_pCAG-PV1-iCre-N270-L1-FRB-NLS-BGHpA

    Plasmid

    #108737

    Purpose

    Expresses N-terminus to aa270 of Cre recombinase fused to FRB CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Cre recombinase fragment N-terminus to aa270
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    FRB,NLS

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPRIME-CMV-GFP-shDyn2

    Plasmid

    #132712

    Purpose

    Encodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat Pdyn gene

    Depositor

    Robert Messing

    Article

    Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

    Insert

    shDyn(2) (Pdyn Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Oct. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMBP_hBrg1 (J1 antibody)

    Plasmid

    #26109

    Depositor

    Jerry Crabtree

    Article

    Wang et al EMBO J. 1996 Oct 1. 15(19):5370-82.

    Insert

    Brg1 (SMARCA4 Human)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 7, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBW2549_pCAG-PV1-VCre-N269-L1-GID1-NLS-BGHpA

    Plasmid

    #108773

    Purpose

    Expresses N-terminus to aa269 of VCre recombinase fused to GID1 CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    VCre recombinase fragment N-terminus to aa269
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    GID1,NLS

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBW2569_pCAG-PV1-GAI-L1-VCre-270C-NLS-BGHpA

    Plasmid

    #108774

    Purpose

    Expresses aa270 to C-terminus of VCre recombinase fused to GAI CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    VCre recombinase fragment aa270 to C-terminus
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    GAI and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PB-BE3-2NLS

    Plasmid

    #119031

    Purpose

    Stably express BE3 in mammalian cells

    Depositor

    Jinsong Li

    Article

    Li et al Nat Cell Biol. 2018 Oct 1. pii: 10.1038/

    Insert

    BE3
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGL3-FLIP pr4

    Plasmid

    #19129

    Depositor

    Wafik El-Deiry

    Article

    Ricci et al Mol Cell Biol. 2004 Oct . 24(19):8541-55

    Insert

    FLIP promoter (CFLAR Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    The sequence corresponds to positions -503 to +10…

    Promoter

    Available since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pTox-EGFPsite1_NGA-PAM (BPK754)

    Plasmid

    #181747

    Purpose

    p11-lacY-wtx1 derivative toxic plasmid encoding an SpCas9 target site for EGFP site 1 with an NGA PAM

    Depositor

    Benjamin Kleinstiver

    Article

    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

    Insert

    ccdB toxin gene and SpCas9 target site (EGFP site 1 NGA PAM)
    

    Use

    Selection plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-Syn-2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)

    Plasmid

    #165492

    Purpose

    Neuronal expression of PACmn with dark Venus, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity

    Depositor

    Thomas Oertner

    Article

    Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

    Insert

    2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)
    

    Use

    AAV

    Tags

    darkVenus

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn1

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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