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pZB604_pT7-sfGFP

Plasmid

#228510

Purpose

Bacterial Hybrid Reporter Plasmid (sfGFP), pT7-sfGFP

Depositor

Timothy Whitehead

Article

Baumer et al ACS Synth Biol. 2024 Dec 20;13(12):4165-

Insert

sfGFP

Use

Synthetic Biology

Promoter

T7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pZB641_pT7_Lysin-KU1

Plasmid

#228511

Purpose

T7 RNAP inducible Lysin KU1 Expression

Depositor

Timothy Whitehead

Article

Baumer et al ACS Synth Biol. 2024 Dec 20;13(12):4165-

Insert

Lysin KU1

Use

Synthetic Biology

Promoter

T7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-a(+)-6xHis-miRFP(-5)-HO1

Plasmid

#219424

Purpose

Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

Depositor

Allie Obermeyer

Article

Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

Inserts

miRFP682
HO1

Tags

MGHHHHHGG

Expression

Bacterial

Mutation

Contains Ribosomal Binding Site for expression of…

Promoter

T7

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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LEGO-pDHS-AP1x6-GM55

Plasmid

#217416

Purpose

Luciferase expression vector with 6 AP-1 sites and the minimal GM-CSF promoter, and a chromatin priming element. Alias: LEGO-GM55-AP-1x6-pDHS(IL-3)

Depositor

Peter Cockerill

Insert

Luciferase, GFP

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

6 AP-1 sites, Human -55 to +28 minimal CSF2 promo…

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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ATG13 sgRNA

Plasmid

#207558

Purpose

pX330 expressing Cas9 and a sgRNA targeting the ATG13 locus

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

ggaaactgatctcaattccc

Expression

Mammalian

Promoter

CMV and U6

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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YP28

Plasmid

#199969

Purpose

Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 28

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

GFP

Expression

Yeast

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pDT4

Plasmid

#199120

Purpose

scrambled promoter circular DNA template

Depositor

Jeff Gelles

Article

Inlow et al Proc Natl Acad Sci U S A. 2023 Jul 11;12

Insert

synthetic transcription template

Expression

Bacterial

Mutation

mutated 5′-CTGGAGTGCG-3′ to 5′-CTGGAGACCG-3′ to …

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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V5-K202R-short-UBASH3A

Plasmid

#192084

Purpose

Mammalian expression of human K202R short-form UBASH3A with V5 tag

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Tags

V5

Expression

Mammalian

Mutation

K202R

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_rSERT_X5C_S277C

Plasmid

#190177

Purpose

For mammalian cell expression of rat SERT and measurement of cytoplasmic pathway accessibility

Depositor

Gary Rudnick

Article

Zhang et al J Biol Chem. 2006 Nov 24;281(47):36213-2

Insert

rat serotonin transporter (Slc6a4 Rat)

Tags

FLAG, His6, and c-myc

Expression

Mammalian

Mutation

Cys15Ala, Cys21Ala, Cys109Ala, Ser277Cys, Cys357I…

Promoter

CMV

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-W279A-short-UBASH3A-Ntag

Plasmid

#192096

Purpose

Human W279A short-form UBASH3A in Gateway pDONR221 (for N-terminal tag)

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Expression

Mammalian

Mutation

W279A

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-K202R-short-UBASH3A-Ntag

Plasmid

#192094

Purpose

Human K202R short-form UBASH3A in Gateway pDONR221 (for N-terminal tag)

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Expression

Mammalian

Mutation

K202R

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-WT-short-UBASH3A-Ctag

Plasmid

#192099

Purpose

Human wild-type short-form UBASH3A in Gateway pDONR221 (for C-terminal tag)

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Expression

Mammalian

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-WT-short-UBASH3A-Ntag

Plasmid

#192100

Purpose

Human wild-type short-form UBASH3A in Gateway pDONR221 (for N-terminal tag)

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Expression

Mammalian

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pET51b_SNAP-ecDHFRmt

Plasmid

#187109

Purpose

Expression of SNAP-ecDHFRmt fusion in bacteria

Depositor

Kai Johnsson

Article

Wang et al Angew Chem Int Ed Engl. 2020 Dec 1;59(49

Insert

SNAP-ecDHFRmt

Tags

Hisx10 and Strep

Expression

Bacterial

Mutation

ecDHFR-R44L-H45Q

Promoter

T7

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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Lmajor_gp63_28_0570_WT

Plasmid

#171642

Purpose

Expression of wildtype L. major glcyoprotein-63 from chromosome 28 in mammalian cells

Depositor

Dennis Ko

Article

Antonia et al PLoS Negl Trop Dis. 2021 Oct 28;15(10):e

Insert

LmjF.28.0570

Tags

Myc-His

Expression

Mammalian

Available Since

Dec. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-AlpJ

Plasmid

#139598

Purpose

expresses AlpJ in E. coli

Depositor

Emily Balskus

Article

Wang et al J Am Chem Soc. 2017 Mar 1;139(8):2864-28

Insert

alpJ

Tags

N terminal hexahistidine

Expression

Bacterial

Promoter

T7

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-KinO1

Plasmid

#139597

Purpose

expresses KinO1 in E. coli

Depositor

Emily Balskus

Article

Wang et al J Am Chem Soc. 2017 Mar 1;139(8):2864-28

Insert

kinO1

Tags

N terminal hexahistidine

Expression

Bacterial

Promoter

T7

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pUG-UBC28

Plasmid

#104693

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

AtUBC28 (UBC28 Mustard Weed)

Use

Synthetic Biology

Mutation

BpiI/BsaI sites removed by point-mutation

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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Yes-10kb

Plasmid

#112996

Purpose

P element vector for transposon insertion of the 10Kb Drosophila Amplicon in Follicle Cells (DAFC-62D) into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Xie et al Proc Natl Acad Sci U S A. 2008 Jul 15;10

Insert

10Kb DAFC-62D amplicon

Use

P insertion vector

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3.1-OPNc PSM1

Plasmid

#106328

Purpose

Mammalian expression of mutant osteopontin

Depositor

Georg Weber

Article

Shen et al Mol Carcinog. 2014 Jun;53(6):480-7. doi:

Insert

OPNc (SPP1 Human)

Expression

Mammalian

Mutation

OPNc phosphorylation site mutant SGSSEEKQNAVSSEET…

Available Since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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