We narrowed to 2,956 results for: NAK
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CMV-mito-GEM-GECO1
Plasmid#32461PurposeMammalian expression of mitochondria-targeting blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imagingDepositorInsertGEM-GECO
UseTagsa duplex of the mitochondrial targeting signal of…ExpressionMammalianMutationGCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…PromoterCMVAvailable sinceDec. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCAG-All-in-one-hCascade
Plasmid#134919PurposeComponents for genome editing in mammalian cells with pPB-CAG-hCas3 and pBS-U6-crRNA-targeted. This plasmid coexpresses PuroR protein.DepositorInsertCascade factors (Cse1(Cas8), Cse2(Cas11), Cas5, Cas6, Cas7) with bpNLS
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVax1-hVEGF165
Plasmid#74466Purposeexpression of hVEGF165 in mammalian cellsDepositorInsertvascular endothelial growth factor 165 (VEGFA Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLX304 YAP1
Plasmid#193689PurposeConstitutive lentiviral expression of YAP1DepositorInsertYAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVAX1-cGAS
Plasmid#196624PurposeExpress mouse cGAS protein in mammalian cells.DepositorInsertcGAS (Cgas Mouse)
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceMarch 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCSIIpuro-mNG-eDHFR(69K6)-RasGEF
Plasmid#172104PurposeMammalian expression of RasGRF1(Cdc25) fused to mNeonGreen-eDHFR(69K6)DepositorInsertmNeonGreen-eDHFR(69K6)-RasGRF1(Cdc25) (RASGRF1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1-alphaAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
p2706-pHAGE-EF1aL-YAP5SA-UBC-GFP
Plasmid#216475Purposelentiviral dual expression of YAP5SA and eGFPDepositorInsertsYAP5SA
eGFP
UseLentiviralTagsExpressionMutationS61A, S109A, S127A, S164A, S381APromoterEF1aL and UBCAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
FLAG-HA-FbxO11-pcDNA3.1-
Plasmid#52501Purposeexpresses human FbxO11 in mammalian cells with FLAG-HA tag at N-terminusDepositorInsertFbxO11 (FBXO11 Human)
UseTagsFLAG-HAExpressionMammalianMutationPromoterCMVAvailable sinceJuly 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS0045
Plasmid#131124PurposeMammalian Target-ACE expression vectorDepositorInsertTadA-linker(32aa)-TadA*(7.10)-linker(32aa)-nCas9-PmCDA1-UGI
UseCRISPRTagsPmCDA1, TadA, and UGIExpressionMammalianMutationA262T, R324L, S409I, E480K, E543D, M694I, E1219V …PromoterCMV promoterAvailable sinceSept. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHes1(467)-luc
Plasmid#41723DepositorInsertHes1 Promoter (-467 to +46) (Hes1 Mouse)
UseLuciferaseTagsFirefly luciferaseExpressionMammalianMutationContains the murine Hes1 promoter (-467 to +46)PromoterHes1 promoter fragment (-467 to +46)Available sinceMarch 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-Crimson
Plasmid#70683PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Crimson reporter from EF-1a promoter. Lentiviral backbone.DepositorInsertsS. pyogenes sgRNA cassette
Crimson Reporter
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEf1-a and hU6Available sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pnEA-vH_His-TEV-SEPT2
Plasmid#174491Purposebacterial expression of human SEPT2DepositorInsertSEPTIN2 (SEPTIN2 Human)
UseTagsHis6-TEVExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Tet-On shYap1-1
Plasmid#193669PurposeTet inducible knockdown of Yap1DepositorInsertYap1 (Yap1 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
Px461-Cas9n-Trp53-sgRNA-beta
Plasmid#88847PurposeCRISPR KO of Trp53DepositorInsertTrp53 (Trp53 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAVTRE3_Clover
Plasmid#135179PurposeAAV expression of TRE(TRE3G)-driven, Clover expression for fluorescent lablingDepositorInsertsClover
TRE3G
UseAAVTagsExpressionMammalianMutationa variant of GFPPromoterTRE3GAvailable sinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mEGFP-CaMKIIa (T286D/T305A/T306A)
Plasmid#127392PurposeFluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286D/T305A/T306A)DepositorInsertcalcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
UseTagsmEGFPExpressionMammalianMutationchanged Threonine 286 to Aspartic acid and Threon…PromoterpCAGAvailable sinceJuly 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
Px461-Cas9n-Trp53-sgRNA-alpha
Plasmid#88846PurposeCRISPR KO of Trp53DepositorInsertTrp53 (Trp53 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Tet-On shYap1-5
Plasmid#193671PurposeTet inducible knockdown of Yap1DepositorInsertYap1 (Yap1 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_ 2xFLAG-2xSTREP_FBXO22
Plasmid#159127Purposeexpresses FBXO22 in mammalian cellsDepositorInsertFBXO22 (FBXO22 Human)
UseTags2X-FLAG _ 2X-STREPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only
