We narrowed to 165,918 results for: addgene

1. pLV-mRFP1-Human UTROPHIN_1-261

   Plasmid

   #225052

   Purpose

   Express in mammalian cells the mRFP1 fused to residues 1-261 of Human Utrophin to detect filamentous actin in live, fix cels or tissues.

   Depositor

   Pantelis Tsoulfas

   Insert

   Human Utrophin residues 1-261 and RFP1 (UTRN Human, Homo Sapiens)
   

   Use

   Lentiviral

   Tags

   Human UTROPHIN 1-261 and mRFP1

   Expression

   Mammalian

   Mutation

   Sequence is derived from ADDGENE Plasmid #26739-R…

   Promoter

   CMV promoter and enhancer

   Available Since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-EGFP-Human-UTROPHIN_1-261

   Plasmid

   #222393

   Purpose

   Express in mammalian cells the EGFP fused to residues 1-261 of Human Utrophin to detect filamentous actin in live, fix cels or tissues.

   Depositor

   Pantelis Tsoulfas

   Insert

   Human Utrophin residues 1-261 and EGFP (UTRN Human, Homo Sapiens)
   

   Use

   Lentiviral

   Tags

   EGFP and Human UTROPHIN 1-261

   Expression

   Mammalian

   Mutation

   Sequence is derived from ADDGENE Plasmid #26737-G…

   Promoter

   CMV promoter and enhancer

   Available Since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. MDC1 KO sgRNA

   Plasmid

   #207103

   Purpose

   pX330 based plasmid for expression of Cas9 and the GGTGTAACGTGGAGCCAGTA sgRNA to target the MDC1 coding sequence.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   GGTGTAACGTGGAGCCAGTA
   

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-PGK-CXCR4 (neo)

   Plasmid

   #192077

   Purpose

   Lentivirus for expression of CXCR4

   Depositor

   Roland Friedel

   Insert

   CXCR4 (CXCR4 Human)
   

   Use

   Lentiviral

   Promoter

   hPGK

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEF-I-GFP GX

   Plasmid

   #45443

   Depositor

   John Brigande

   Article

   Gubbels et al Nature. 2008 Sep 25. 455(7212):537-41.

   Type

   Empty backbone

   Tags

   IRES EGFP

   Expression

   Mammalian

   Promoter

   human EF1α

   Available Since

   July 2, 2013

   Availability

   Academic Institutions and Nonprofits only

6. p8267 LentiCRISPR v2 hygro sgNT-2

   Plasmid

   #193978

   Purpose

   Expression of spCas9 and non-targeting control sgRNA

   Depositor

   Elizabeth White

   Article

   Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

   Insert

   spCas9
   

   Use

   Lentiviral

   Available Since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLVPRT-tTR-KRAB

   Plasmid

   #11648

   Purpose

   Tet-regulated (Tet-on) lentiviral vector for transgene (hPrion promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

   Depositor

   Didier Trono, Patrick Aebischer

   Article

   Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

   Insert

   hPrion, GFP, tTR-KRAB, Tet-on
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pdCas9-NED

   Plasmid

   #109358

   Purpose

   Mammalian vector for expressing effector domains fused to dCas9.

   Depositor

   Antal Kiss

   Article

   Goubert et al Methods Mol Biol. 2018;1767:395-415. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   dCas9, SV40 NLS, 3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX459_gRNA-AAVS1_hspCas9

   Plasmid

   #193309

   Purpose

   Cas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

   Insert

   Cas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   not applicable

   Available Since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-CarO-BFP2-TSERex

    Plasmid

    #124795

    Purpose

    Expresses the proton pump rhodopsin in mammalian cells under CAG promoter

    Depositor

    Yulong Li

    Article

    Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

    Insert

    CarO-BFP2
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-Mac-BFP2-TSERex

    Plasmid

    #124793

    Purpose

    Expresses the proton pump rhodopsin in mammalian cells under CAG promoter

    Depositor

    Yulong Li

    Article

    Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

    Insert

    Mac-BFP2
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. DNA-PKcs N-terminal sgRNA

    Plasmid

    #207093

    Purpose

    pX330 based plasmid for expression of Cas9 and the AGCGGGACTCGGCGGCATGG sgRNA to target the DNA-PKcs locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    AGCGGGACTCGGCGGCATGG
    

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. 7TG

    Plasmid

    #24314

    Depositor

    Roel Nusse

    Article

    Brugmann et al Development. 2007 Sep . 134(18):3283-95.

    Insert

    7xTcf-eGFP
    

    Use

    Lentiviral

    Mutation

    EGFP is GFP with S65T and F64L mutations

    Available Since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLentiCRISPRv2-Stuffer-HepmCherry

    Plasmid

    #192825

    Purpose

    Contains stuffer sequence into which sgRNAs can be cloned and expresses mCherry from hepatocyte-specific promoter

    Depositor

    Kristin Knouse

    Article

    Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

    Insert

    mCherry
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    Hepatocyte-specific promoter (HS-CRM8-TTRmin modu…

    Available Since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti UBC hNLRP3-mNG

    Plasmid

    #218642

    Purpose

    Expresses NLRP3 tagged with mNeonGreen in mammalian cells (lentiviral); driven by the weak UBC promoter with puro selection

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    NLRP3 (NLRP3 Human)
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Promoter

    UBC

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. ULK1 sgRNA

    Plasmid

    #207559

    Purpose

    pX330 expressing Cas9 and a sgRNA targeting the ULK1 locus

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    CCAGCCAGGCCAGAAAGGTC
    

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVPT-rtTR-KRAB

    Plasmid

    #11777

    Purpose

    Tet-regulated (Tet-off) lentiviral vector for transgene (mPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

    Depositor

    Didier Trono, Patrick Aebischer

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Insert

    mPGK, GFP, rtTR-KRAB, Tet-off
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSNAPf-Nup43

    Plasmid

    #98276

    Purpose

    mammalian expression of SNAPf-Nup43

    Depositor

    Mike Heilemann

    Article

    Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

    Insert

    Nup43 (NUP43 Human)
    

    Tags

    SNAPf-tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. 6BHD (SETDB1)

    Plasmid

    #119955

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Insert

    SETDB1 (SETDB1 Human)
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    contains amino acids 190-410

    Promoter

    T7

    Available Since

    Feb. 1, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. ATG9A sgRNA

    Plasmid

    #207557

    Purpose

    pX330 expressing Cas9 and a sgRNA targeting the ATG9A locus

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    CACTGAATACCAGCGCCTAG
    

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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