We narrowed to 7,508 results for: ef1a

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pAF125

Plasmid

#222373

Purpose

Recombinant human IgG antibody vector without variable regions. Contains mTagBFP marker.

Depositor

Andrew S. Flies

Article

Slyp et al Methods Mol Biol. 2024;2826:231-249. doi

Insert

IGHG (partial)

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pAF146

Plasmid

#222377

Purpose

Express Tasmanian devil PDL1 protein (saha_PDL1_FL_DN11026_c0_g1_i1)

Depositor

Andrew S. Flies

Article

Slyp et al Methods Mol Biol. 2024;2826:231-249. doi

Insert

PDL1

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pKER-Clover

Plasmid

#85974

Purpose

Expresses Clover from the EF1a promoter in mammalian cells

Depositor

Michele Calos

Article

Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

Insert

Clover

Expression

Mammalian

Promoter

Ef-1a

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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rtTA-N144

Plasmid

#66810

Purpose

2nd generation lentiviral transfer plasmid. Expresses the reverse tetracycline-controlled transactivator (rtTA) with hygromycin resistance

Depositor

Andrew Yoo

Article

Richner et al Nat Protoc. 2015 Oct;10(10):1543-55. doi

Insert

Reverse tetracycline-controlled transactivator

Use

Lentiviral

Expression

Mammalian

Promoter

Human EF1a

Available Since

Sept. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-R-Creon shRNA[Control]

Plasmid

#180775

Purpose

This vector simultaneously expresses mCherry protein and shRNA in a Cre-dependent manner.

Depositor

Eun Mi Hwang

Article

Kim et al J Neurosci Methods. 2021 Dec 21:109452.

Type

Empty backbone

Use

AAV, Cre/Lox, and RNAi

Expression

Mammalian

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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cEF.tk-GFP

Plasmid

#33308

Depositor

Linzhao Cheng

Article

Pomper et al Cell Res. 2009 Mar;19(3):370-9.

Insert

HSV-TK/GFP

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

March 29, 2012

Availability

Academic Institutions and Nonprofits only

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LentiSAMPHv2

Plasmid

#167934

Purpose

All-in-one lentiviral CRISPRa gRNA expression with blasticidin selection marker.

Depositor

Ronald Germain

Article

Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6, EF1a

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pGEMS-II

Plasmid

#204765

Purpose

GEMS sensor system (pCMV-EGFP-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577)

Depositor

Kate Meyer

Article

Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

EGFP-m6Alinker-DHFR

Mutation

mutated all RACs in EGFP to RGC/RCC

Promoter

CMV

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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PB-TO-hNGN2-BSD-mApple

Plasmid

#182308

Purpose

Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression, bsd selection, mApple

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Inserts

hNGN2 (NEUROG2 Human)
bsd-T2A-mycNLS-mApple

Tags

T2A-mycNLS-mApple

Expression

Mammalian

Promoter

EF1a and TRE3G

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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CROPseq-puro-v2

Plasmid

#127458

Purpose

Entry plasmid for guide cloning

Depositor

Paul Blainey

Article

Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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pYSEAP

Plasmid

#37326

Depositor

John Schiller

Article

Pastrana et al Virology. 2004 Apr 10;321(2):205-16.

Insert

SEAP

Expression

Mammalian

Promoter

EF1a

Available Since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

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LentiGuide-TagRFP-2A-BSD

Plasmid

#167930

Purpose

Lentiviral gRNA expression with TagRFP fluorescence and blasticidin selection marker.

Depositor

Ronald Germain

Article

Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6, EF1a

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-Cre-MS2_UbC-NLS-tdMCP-GFP (Cre_tdMCP-GFP)

Plasmid

#219537

Purpose

a. Expresses Cre recombinase tagged with 23x repeats of MS2 binding sequence under EF1A promotor and b. NLS-tdMCP_GFP reporter under UbC promotor

Depositor

Jeffrey Gerst

Article

Haimovich et al bioRxiv 2024.11.06.622258

Inserts

Cre recombinase (cre )
NLS-tdMCP-GFP

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 11, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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p11L1w

Plasmid

#37330

Depositor

John Schiller

Article

Mossadegh et al Virology. 2004 Aug 15;326(1):57-66.

Insert

HPV11 L1

Expression

Mammalian

Promoter

EF1a

Available Since

July 23, 2013

Availability

Academic Institutions and Nonprofits only

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pDD426

Plasmid

#202079

Purpose

Frame +1/-0 selector for NHEJ knock-in

Depositor

Daniel Dickinson

Article

Shi et al Development. 2023 May 15;150(10):dev2013

Insert

pEF1a-mCherry-Cas9 + Frame +1/-0 sgRNA

Use

CRISPR

Promoter

EF1a

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pDD425

Plasmid

#202078

Purpose

Frame +0 selector for NHEJ knock-in

Depositor

Daniel Dickinson

Article

Shi et al Development. 2023 May 15;150(10):dev2013

Insert

pEF1a-mCherry-Cas9 + Frame +0 sgRNA

Use

CRISPR

Promoter

EF1a

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pDD427

Plasmid

#202080

Purpose

Frame +2/-1 selector for NHEJ knock-in

Depositor

Daniel Dickinson

Article

Shi et al Development. 2023 May 15;150(10):dev2013

Insert

pEF1a-mCherry-Cas9 + Frame +2/-1 sgRNA

Use

CRISPR

Promoter

EF1a

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pYS19

Plasmid

#202084

Purpose

Frame -2 selector for NHEJ knock-in

Depositor

Daniel Dickinson

Article

Shi et al Development. 2023 May 15;150(10):dev2013

Insert

pEF1a-mCherry-Cas9 + Frame –2 sgRNA

Use

CRISPR

Promoter

EF1a

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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LentiGuide-TagBFP-2A-BSD

Plasmid

#167929

Purpose

Lentiviral gRNA expression with TagBFP fluorescence and blasticidin selection marker.

Depositor

Ronald Germain

Article

Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6, EF1a

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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PCT62

Plasmid

#170391

Purpose

expresses three sgRNA cassettes

Depositor

Jonathan Weissman

Article

Quinn et al Science. 2021 Feb 26;371(6532). pii: sci

Insert

PCT62-targetsite

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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