We narrowed to 7,783 results for: ef1a

1. FH-Tet2-pEF

   Plasmid

   #41710

   Purpose

   This plasmid expresses mouse Tet2 with N-terminal FLAG and HA tags in a mammalian system

   Depositor

   Anjana Rao

   Article

   Ko et al Nature. 2010 Dec 9;468(7325):839-43. doi

   Insert

   tet methylcytosine dioxygenase 2 (Tet2 Mouse)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLX_TRC311 ETV4-L

   Plasmid

   #74982

   Purpose

   Constitutive ETV4 ORF, blasticidin selection

   Depositor

   William Hahn

   Article

   Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

   Insert

   ETV4 (ETV4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S396N

   Promoter

   EF1a

   Available since

   July 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. ALCAM-p2A-E2Crimson

   Plasmid

   #83939

   Purpose

   Lentiviral vector expressing ALCAM-p2A-E2Crimson

   Depositor

   David Sabatini

   Article

   Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

   Insert

   ALCAM (ALCAM Human)
   

   Use

   Lentiviral

   Tags

   p2A-E2Crimson

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pZac2.1 EFs-SaCas9-U6-Sa DMDR7-U6-SaDMDL2

   Plasmid

   #78607

   Purpose

   A single vector AAV-Cas9 system containing SaCas9 under EFs promoter, gRNAs targeting Dmd introns 22 and 23

   Depositor

   Amy Wagers

   Article

   Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

   Insert

   EFs-SaCas9-U6-Sa DMDR7-U6-SaDMDL2
   

   Use

   AAV and CRISPR

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a-short; U6

   Available since

   Sept. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

5. pEF-Bos TRAM Flag

   Plasmid

   #41551

   Depositor

   Kate Fitzgerald, Doug Golenbock

   Article

   Fitzgerald et al J Exp Med. 2003 Oct 6;198(7):1043-55. Ep

   Insert

   TRAM (TICAM2 Human)
   

   Use

   Tags

   6xHis and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 14, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pJFA344C7

   Plasmid

   #49236

   Purpose

   TET1-catalytic domain (CD) expression vector for TALE-TET1 fusion protein to induce targeted demethylation in mammalian cells

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

   Insert

   TALE-TET1CD Backbone (TET1 Synthetic, Human)
   

   Use

   TALEN; Tale-tet1 expression vector

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJOP-HTT-HR18Q

   Plasmid

   #87228

   Purpose

   HTT HR arms with 18 CAG repeats and piggyBac selection cassette

   Depositor

   Mahmoud Pouladi

   Article

   Xu et al Stem Cell Reports. 2017 Feb 21. pii: S22

   Insert

   1.7kb HTT 5' homology arm, 2.5kb HTT 3' homology arm
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a driving selection cassette

   Available since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHAGE GFP-Ago2 S387A

   Plasmid

   #60654

   Purpose

   GFP-Ago2 S387A mutant lentiviral vector

   Depositor

   Carl Novina

   Article

   Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

   Insert

   Argonaute 2 (AGO2 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mutation

   S387A

   Promoter

   EF1a

   Available since

   May 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJFA334E9

   Plasmid

   #49237

   Purpose

   TET1-catalytic domain (CD) expression vector for TALE-TET1 fusion protein to induce targeted demethylation in mammalian cells

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

   Insert

   TET1 tet methylcytosine dioxygenase 1 (TET1 Synthetic, Human)
   

   Use

   TALEN; Tale-tet1 expression vector

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJFA274

    Plasmid

    #49239

    Purpose

    TET1-catalytic domain (CD) expression vector for TALE-TET1 fusion protein to induce targeted demethylation in mammalian cells

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    TET1 tet methylcytosine dioxygenase 1 (TET1 Synthetic, Human)
    

    Use

    TALEN; Tale-tet1 expression vector

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEF5/FRT/DEST-3xHA/mGli3/Flag P1-6A

    Plasmid

    #51248

    Purpose

    Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907,980,1006 mutated to Ala

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli3 (Gli3 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV and Flag

    Expression

    Mammalian

    Mutation

    amino acids 849,865,877,907,980,1006 mutated to A…

    Promoter

    EF1a

    Available since

    March 7, 2014

    Availability

    Academic Institutions and Nonprofits only

12. MLM3739

    Plasmid

    #49959

    Purpose

    Expresses a mutant TALE-TET1CD (inactive catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene for use as a control

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    3x Flag Tet1CDmut HB-4 (HBB Human)
    

    Use

    TALEN

    Tags

    3x FLAG

    Expression

    Mutation

    catalytic domain of TET1 inactive (H1671Y and D16…

    Promoter

    EF1a

    Available since

    Dec. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

13. Exp-pcDNA3.2delCMV(EF1α-tTA/TetO-mCh-Rs1)

    Plasmid

    #26797

    Depositor

    Edward Hsiao

    Article

    Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

    Insert

    EF1a-tTA/TetO-mCherry-P2A-Rs1 (HTR4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Human 5HT4B receptor with D100A mutation, generat…

    Promoter

    Available since

    Feb. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. MLM3720

    Plasmid

    #49944

    Purpose

    Expresses a mutant TALE-TET1CD (inactive catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene for use as a control

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    3x FLAG Tet1CDmut RH-3 (RHOXF2 Human)
    

    Use

    TALEN

    Tags

    3x FLAG

    Expression

    Mutation

    catalytic domain of TET1 inactive (H1671Y and D16…

    Promoter

    EF1a

    Available since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

15. MLM3709

    Plasmid

    #49943

    Purpose

    Expresses a 3x Flag TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    3x FLAG Tet1CD RH-3 (RHOXF2 Human)
    

    Use

    TALEN

    Tags

    3x FLAG

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pEF5/FRT/DEST-3xHA/mGli2 Pc-gA/P1-6A

    Plasmid

    #51257

    Purpose

    Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248,789,805,817,848,923,956 mutated to Ala

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli2 (Gli2 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV

    Expression

    Mammalian

    Mutation

    amino acids 216,220,224,230,248,789,805,817,848,9…

    Promoter

    EF1a

    Available since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

17. MLM3743

    Plasmid

    #49962

    Purpose

    Expresses a mutant TALE-TET1CD (inactive catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene for use as a control

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    3x Flag Tet1CDmut HB-5 (HBB Human)
    

    Use

    TALEN

    Tags

    3x FLAG

    Expression

    Mutation

    catalytic domain of TET1 inactive (H1671Y and D16…

    Promoter

    EF1a

    Available since

    Dec. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

18. MLM3747

    Plasmid

    #49965

    Purpose

    Expresses a mutant TALE-TET1CD (inactive catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene for use as a control

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    3x Flag Tet1CDmut HB-6 (HBB Human)
    

    Use

    TALEN

    Tags

    3x FLAG

    Expression

    Mutation

    catalytic domain of TET1 inactive (H1671Y and D16…

    Promoter

    EF1a

    Available since

    Dec. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pJFA345D4

    Plasmid

    #49238

    Purpose

    TET1-catalytic domain (CD) expression vector for TALE-TET1 fusion protein to induce targeted demethylation in mammalian cells

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    TET1 tet methylcytosine dioxygenase 1 (TET1 Synthetic, Human)
    

    Use

    TALEN; Tale-tet1 expression vector

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MLM3713

    Plasmid

    #49946

    Purpose

    Expresses a 3x Flag TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    3x FLAG Tet1CD RH-4 (RHOXF2 Human)
    

    Use

    TALEN

    Tags

    3x FLAG

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 31, 2013

    Availability

    Academic Institutions and Nonprofits only