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pABY-c25

Plasmid

#201769

Purpose

Expresses mNeonGreen-tagged NbALFA under CYC1 promoter in yeast

Depositor

Saravanan Palani

Publication

Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

Insert

NbALFA

Tags

40aa Linker, mNeonGreen

Expression

Yeast

Promoter

CYC1

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-6-IPT-9

Plasmid

#197746

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Publication

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

Isopentyl transferase (IPT)

Expression

Plant

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pYSD313

Plasmid

#212931

Purpose

Encodes the Aga2 signal peptide, mRuby2 fluorescent protein HA-tagged Sed1p yeast surface display anchor protein with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

Depositor

Paul Young

Publication

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

Sed1 (SED1 Budding Yeast)

Expression

Yeast

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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mc2 neo

Plasmid

#206214

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Publication

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-9-35S-11

Plasmid

#197759

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Publication

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

35S terminator

Expression

Plant

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pFTK055

Plasmid

#171327

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Publication

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

rTA2S-M2 (TetR-3xVP16) transcriptional activator

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pPD292 ZF43x8-Compact_EYFP

Plasmid

#138903

Purpose

EYFP reporter vector for the ZF43x8-Compact promoter

Depositor

Joshua Leonard

Publication

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x8-Compact

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x8-Compact

Available since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pPD558 ZF172x6-Compact_EYFP

Plasmid

#138872

Purpose

EYFP reporter vector for the ZF172x6-Compact promoter

Depositor

Joshua Leonard

Publication

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF172x6-Compact

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF172x6-Compact

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pPD547 ZF43x6-Compact_EYFP

Plasmid

#138861

Purpose

EYFP reporter vector for the ZF43x6-Compact promoter

Depositor

Joshua Leonard

Publication

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x6-Compact

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x6-Compact

Available since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pPD541 ZF13x6-Compact_EYFP

Plasmid

#138855

Purpose

EYFP reporter vector for the ZF13x6-Compact promoter

Depositor

Joshua Leonard

Publication

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF13x6-Compact

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF13x6-Compact

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pJW1883

Plasmid

#154331

Purpose

sgRNA Target Vector for CRISPR

Depositor

Jordan Ward

Publication

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

ttTi5605 site targeting sgRNA (F+E) with R07E5.16 U6 promoter and 3'UTR

Use

CRISPR

Expression

Worm

Mutation

F+E mutations in sgRNA

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1849

Plasmid

#154327

Purpose

sgRNA Target Vector for CRISPR

Depositor

Jordan Ward

Publication

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

ttTi4348 site targeting sgRNA (F+E) with K09B11.2 U6 promoter and 3'UTR

Use

CRISPR

Expression

Worm

Mutation

F+E mutations in sgRNA

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pBBR1_DVK_AE(J23100-RiboJ-UTR1-E0040m-B0015)

Plasmid

#197662

Purpose

Expresses GFP under the control of J23100 and RiboJ-UTR1

Depositor

Eric Young

Publication

Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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Tol2pA2-drl:APEX2-mCherry

Plasmid

#188945

Purpose

Plasmid for Tol2 transgenesis expressing APEX2 tagged to mitochondria and nuclei, and cytoplasmic mCherry, driven by the draculin promoter

Depositor

Owen Tamplin

Publication

Agarwala et al Elife. 2022 Aug 9;11. pii: 64835. doi: 1

Insert

drl:mito-APEX2_p2A_APEX2-H2B_p2A_mCherry_polyA

Use

Tol2 destination vector (attr4-r3)

Available since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_KRAB_KanR neo

Plasmid

#167877

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB

Depositor

Alejandro Chavez, Nan Cher Yeo

Publication

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pPD057 VP64-ZF37

Plasmid

#138834

Purpose

Constitutive expression of VP64-ZF37 under the CMV promoter.

Depositor

Joshua Leonard

Publication

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP64-ZF37

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pPD536 VP16-ZF150

Plasmid

#138769

Purpose

Constitutive expression of VP16-ZF150 under the CMV promoter.

Depositor

Joshua Leonard

Publication

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP16-ZF150

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pPD538 VP16-ZF172

Plasmid

#138772

Purpose

Constitutive expression of VP16-ZF172 under the CMV promoter.

Depositor

Joshua Leonard

Publication

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP16-ZF172

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pStrep-2xEGFP-N1

Plasmid

#122489

Purpose

Expression of Strep-tagged 2xEGFP

Depositor

Georg Mayr

Publication

Zweifel et al Anal Biochem. 2019 Sep 12:113430. doi: 1

Insert

Strep-tagged 2xEGFP

Expression

Mammalian

Promoter

CMV

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pStrep-3xEGFP-N1

Plasmid

#122490

Purpose

Expression of Strep-tagged 3xEGFP

Depositor

Georg Mayr

Publication

Zweifel et al Anal Biochem. 2019 Sep 12:113430. doi: 1

Insert

Strep-tagged 3xEGFP

Expression

Mammalian

Promoter

CMV

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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