We narrowed to 300 results for: nisch

1. TetO-FUW-PLAGL1

   Plasmid

   #61542

   Purpose

   Expresses PLAGL1 in mammalian cells

   Depositor

   Rudolf Jaenisch

   Article

   Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

   Insert

   PLAGL1 (PLAGL1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

2. FUW-TetO-Dppa2

   Plasmid

   #40799

   Depositor

   Rudolf Jaenisch

   Article

   Buganim et al Cell. 2012 Sep 14;150(6):1209-22. doi: 1

   Insert

   Dppa2 (Dppa2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Minimal CMV + TetO

   Available since

   Oct. 12, 2012

   Availability

   Academic Institutions and Nonprofits only

3. TetO-FUW-Hes5

   Plasmid

   #61536

   Purpose

   Expresses Hes5 in mammalian cells

   Depositor

   Rudolf Jaenisch

   Article

   Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

   Insert

   Hes5 (Hes5 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pAC5-dual-dCas9VP48-sgTetO

   Plasmid

   #48237

   Purpose

   Dual expression construct expressing both dCas9VP48 and sgTetO from separate promoters

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9VP48 and sgTetO
   

   Use

   CRISPR

   Tags

   HA-tag and VP48

   Expression

   Mammalian

   Mutation

   D10A H840A (catalytically inactive)

   Promoter

   Available since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3 PBD wt (Nigg AH12)

   Plasmid

   #41162

   Depositor

   Erich Nigg

   Article

   Hanisch et al Mol Biol Cell. 2006 Jan;17(1):448-59. Ep

   Insert

   PBD (PLK1 Human)
   

   Use

   Tags

   3xMyc and PreScission Site

   Expression

   Mammalian

   Mutation

   Contains Plk1-PBD only (aa 326–603)

   Promoter

   CMV

   Available since

   June 7, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pAC92-pmax-dCas9VP96

   Plasmid

   #48224

   Purpose

   dCas9VP96 on pmax expression vector

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9(D10A;H840A) fusion with VP96 activation domain
   

   Use

   CRISPR

   Tags

   HA Tag and VP96

   Expression

   Mammalian

   Mutation

   D10A;H840A

   Promoter

   CAGGS

   Available since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. Nanog-luciferase targeting construct

   Plasmid

   #26591

   Depositor

   Rudolf Jaenisch

   Article

   Lyssiotis et al Proc Natl Acad Sci U S A. 2009 Jun 2. 10

   Insert

   Nanog promoter-luciferase-lox-pgk-neomycin-loc
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3 PBD HK-AA (Nigg AH13)

   Plasmid

   #41161

   Depositor

   Erich Nigg

   Article

   Hanisch et al Mol Biol Cell. 2006 Jan;17(1):448-59. Ep

   Insert

   PBD HK-AA (PLK1 Human)
   

   Use

   Tags

   3xMyc and PreScission Site

   Expression

   Mammalian

   Mutation

   Contains Plk1-PBD only (aa 326–603); HK-AA (H538A…

   Promoter

   CMV

   Available since

   June 7, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pAC1-pCR8-dCas9VP48

   Plasmid

   #48214

   Purpose

   dCas9VP48 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9(D10A;H840A) fusion with VP48 activation domain
   

   Use

   CRISPR; Gateway donor

   Tags

   HA Tag and VP48

   Expression

   Mutation

   D10A;H840A

   Promoter

   Available since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. Fuw-dCas9-Dnmt3a-P2A-tagBFP

    Plasmid

    #84569

    Purpose

    Lentiviral construct to express dCas9 fused with Dnmt3a-P2A-tagBFP

    Depositor

    Rudolf Jaenisch

    Article

    Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

    Insert

    dCas9-Dnmt3a (DNMT3A Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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