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Plasmid#227012PurposeLentiviral Expression of dCas9-KRAB from S. uberis fused to the repressive KRAB domainDepositorInsertdCas9
UseCRISPR and LentiviralTags3xFLAG, SV40 NLSExpressionMutationPromoterUbCAvailable sinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMTgR2
Plasmid#107286PurposePlasmid can be used for Drosophila S2 driven secretion of extracellular part of human interferon-_ receptor 2.DepositorInsertInterferon gamma receptor 2 (IFNGR2 Human)
UseTagsCATCATCACCATCACCATGAExpressionInsectMutationPromoterMetallothionein promoterAvailable sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-NC-CRISPRi (Gen3)
Plasmid#73499PurposeConstitutive CRISPR interference (CRISPRi) knock in construct into the AAVS1 locusDepositorInsertdCas9-KRAB
UseCRISPRTagsHA, KRAB, and NLSExpressionMammalianMutationD10A, H840A (catalytically deactivated Cas9)PromoterCAGAvailable sinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-αIFNα-ab
Plasmid#110799PurposeExpression and secretion of an scFv antibody (clone) 4EA1 against all mouse interferon α subtypes in mammalian cellsDepositorInsertantibody against mouse interferon alpha
UseTagsER signal peptide, His6 tag, and myc epitope tagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-αIFNα-ib
Plasmid#110798PurposeExpression of an intrabody derived from the monoclonal antibody 4EA1 against all mouse interferon α subtypes in mammalian cellsDepositorInsertintrabody against mouse interferon alpha
UseTagsER retention signal (SEKDEL), ER signal peptide, …ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-NDi-CRISPRi (Gen1)
Plasmid#73497PurposeDox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus with mCherry markerDepositorInsertsdCas9-KRAB-P2A-mCherry
rtTA
UseCRISPRTagsHA, KRAB, and NLSExpressionMammalianMutationD10A, H840A (catalytically deactivated Cas9)PromoterCAG and TRE3GAvailable sinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-NDi-CRISPRi (Gen2)
Plasmid#73498PurposeDox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locusDepositorInsertsdCas9-KRAB
rtTA
UseCRISPRTagsHA, KRAB, and NLSExpressionMammalianMutationD10A, H840A (catalytically deactivated Cas9)PromoterCAG and TRE3GAvailable sinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
TFORF3084
Plasmid#144560PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZFP36L1 (ZFP36L1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0727
Plasmid#141674PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZFP36L2 (ZFP36L2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJL31
Plasmid#227428PurposeKanamycin resistant L5-integrative vector for CRISPR interference in M.smegmatis with constitutive expression of mWasabi under the control of the psmyc promotor (psmyc::mWasabi)DepositorInsertmWasabi
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJL32
Plasmid#227429PurposeKanamycin resistant L5-integrative vector for CRISPR interference in M.smegmatis with the constitutive expression of tdTomato under the control of the psmyc promotor (psmyc::tdTomato)DepositorInserttdTomato
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJL33
Plasmid#227430PurposeKanamycin resistant L5-integrative vector for CRISPR interference in M.tuberculosis with the constitutive expression of mWasabi under the control of the psmyc promotor (psmyc::mWasabi)DepositorInsertmWasabi
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJL34
Plasmid#227431PurposeKanamycin resistant L5-integrative vector for CRISPR interference in M.tuberculosis with the constitutive expression of tdTomato under the control of the psmyc promotor (psmyc::tdTomato)DepositorInserttdTomato
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pQE-60NA-msGFP2
Plasmid#135301PurposeEncodes a photostable monomeric superfolder GFPDepositorInsertmsGFP2
UseTagsExpressionBacterialMutationPromoterT5Available sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFLAG GLTPD2
Plasmid#170745PurposeThe plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.DepositorInsertGLTPD2 (GLTPD2 Human)
UseTagsFLAGExpressionMammalianMutationV49F, V152A and D209E- Please see depositor comme…PromoterAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pQE-60NA-proinsulin-msGFP2
Plasmid#160466PurposeEncodes a fusion of human proinsulin and the monomeric superfolder GFP2DepositorInsertproinsulin-msGFP2
UseTagsExpressionBacterialMutationPromoterT5Available sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZ8-T_dCas9
Plasmid#74062PurposepZ8-1 plasmid carrying dcas9, driven by the IPTG-inducible Ptac promoter, KanRDepositorInsertdcas9
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterptacAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQE-60NA-proinsulin-mEGFP
Plasmid#160464PurposeEncodes a fusion of human proinsulin and the monomeric enhanced EGFP. This construct can be used as a negative control for superfolder activity in bacteriaDepositorInsertproinsulin-mEGFP
UseTagsExpressionBacterialMutationPromoterT5Available sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMAH-pnGFP-Ultra
Plasmid#157924PurposePeroxynitrite sensor pnGFP-Ultra in mammalian cells (use with pMAH-POLY-eRF1(E55D))DepositorInsertpnGFP-Ultra
UseTagsExpressionMammalianMutationPromotercmvAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only