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pCMV-deltaR8.2(A14C,E45C)
Plasmid#79047Purposemammalian expression, lentiviral packagingDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJK438
Plasmid#71590PurposeProduces Thermoplasma acidophilum citrate synthase with C-terminal His6 tag (TpCSH6)DepositorInsertcitrate synthase
UseTagsHis6ExpressionBacterialMutationPromoterT7 promoterAvailable sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK240
Plasmid#71127PurposeExpression of Acetobacter aceti 1023 citrate synthase (AaCS) in E. coliDepositorInsertcitrate synthase
UseTagsGyrA intein and His6ExpressionBacterialMutationPromoterT7Available sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-deltaR8.2(A14C,E45C,A92E)
Plasmid#79048Purposemammalian expression, lentiviral packagingDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVP2del50
Plasmid#125185PurposeExpresses the VP2del50 truncation mutant protein derived from AAV2 VP2DepositorInserthexahistidine tag
UseTagsExpressionMammalianMutationInserted start codon and hexahistidine tag at 2697PromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVP2del30
Plasmid#125184PurposeExpresses the VP2del30 truncation mutant protein derived from AAV2 VP2DepositorInserthexahistidine tag
UseTagsExpressionMammalianMutationInserted start codon and hexahistidine tag at 2637PromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-EGFP-NLS-P2A-mCherry-PTS1
Plasmid#87803PurposeMammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using P2A. NLS and PTS1 can be replaced by proteins of interest.DepositorInsertsNLS
PTS1
UseTagsEGFP and mCherryExpressionMammalianMutationPromoterCMV promoter, tetracycline operatorAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-EGFP-NLS-P2AT2A-mCherry-PTS1
Plasmid#87828PurposeHigh-efficient mammalian expression vector for co-expression of EGFP-tagged and mCherry-tagged proteins using P2AT2A. NLS and PTS1 can be replaced by proteins of interest.DepositorInsertsNLS
PTS1
UseTagsEGFP and mCherryExpressionMammalianMutationPromoterCMV promoter, tetracycline operatorAvailable sinceApril 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-EGFP-NLS-T2A-mCherry-PTS1
Plasmid#87827PurposeMammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using T2A. NLS and PTS1 can be replaced by proteins of interest.DepositorInsertsNLS
PTS1
UseTagsEGFP and mCherryExpressionMammalianMutationPromoterCMV promoter, tetracycline operatorAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pnCS_mEGFP-DSep2_Peanut-Strep
Plasmid#174496Purposebacterial co-expression of Drosophila Sep2 fused to monomeric EGFP and of Drosophila PeanutDepositorUseTagsStrep and mEGFPExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDF11-SFPQ(214-598)_quadruple_mutant
Plasmid#135441PurposeExpresses SFPQ 214-598 quadruple mutant (L535A, L539A, L546A, M549A) with TEV-cleavable hexahistidine tagDepositorInsertsplicing factor proline and glutamine rich (SFPQ Human)
UseTagsHexahistidineExpressionBacterialMutationL535A, L539A, L546A, M549APromoterT7Available sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-OSF-TRIM5alpha(African green monkey pygerythrus)(1-293)
Plasmid#79030PurposeBaculoviral entry vector to produce Bac-to-bac baculovirusesDepositorInsertOSF-TRIM5alpha(African green monkey pygerythrus)(1-293)
UseTagsOneSTrEP-FLAGExpressionInsectMutationdeleted amino acid 294-515 from frican green monk…PromoterpolyhedrinAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher002-oHex
Plasmid#124670PurposeExpresses SpyCatcher002-oHex for hexamerization of SpyTag-proteins via hexameric coiled-coilDepositorInsertSpyCatcher002-oHex
UseTagsC-tag, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET11a-CA(A14C, E45C)
Plasmid#79045PurposeBacterial vector for cloning and propagation in DH5alphaDepositorInsertCA (A14C, E45C)
UseTagsExpressionBacterialMutationA14C and E45C mutations in HIV-1 CA proteinPromoterT7Available sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJG80
Plasmid#89282PurposeEntry vector containing TaCas9-nos terminator between attL1 and attR5 sitesDepositorInsertTaCas9
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-OSF-TRIM5-21R
Plasmid#79063PurposeGateway entry vector for cloning and propagation in DH5alphaDepositorInsertOSF-TRIM5-21R
UseTagsOneSTrEP-FLAGExpressionInsectMutationPromoterT7Available sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET11a-CA(A14C, E45C, A92E)
Plasmid#79046PurposeBacterial vector for cloning and propagation in DH5alphaDepositorInsertCA (A14C, E45C, A92E)
UseTagsExpressionBacterialMutationA14C, E45C, A92E mutations in HIV-1 CA proteinPromoterT7Available sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJK511
Plasmid#71591PurposeProduces Thermoplasma acidophilum citrate synthase with C-terminal His6 tag, His222>Gln mutant (TpCSH6-H222Q)DepositorInsertcitrate synthase
UseTagsHis6ExpressionBacterialMutationchanges histidine-222 to glutaminePromoterT7 promoterAvailable sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-OSF-TRIM5-21R(1-300)
Plasmid#79029PurposeGateway entry vector for cloning and propagation in DH5alphaDepositorInsertTRIM5-21R (1-300)
UseTagsOneSTrEP-FLAGExpressionInsectMutationPromoterT7Available sinceMarch 3, 2017AvailabilityAcademic Institutions and Nonprofits only
