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Plasmid#50976PurposeAAV transfer vector with mammalian codon-optimized ChD-citrine between AAV2 ITRDepositorInsertoChD-citrine
UseAAVTagscitrineExpressionMutationChD gene is mammalian codon optimizedPromoterhuman synapsin promoterAvailable sinceFeb. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
MsACR1-pmCerulean3-N1
Plasmid#204958PurposeExpression of channelrhodopsin MsACR1 fused to mCerulean3 in mammalian cellsDepositorInsertMsACR1
UseTagsmCerulean3ExpressionMammalianMutationPromoterCMV (+enhancer)Available sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28-At_PRORP1-His
Plasmid#67869Purposebacterial expression of PRORP1 (At2g32230), full coding sequence (mitoch./chloropl. form) + C-term. His tagDepositorInsertPRORP1 (PRORP1 Mustard Weed)
UseTagsHisExpressionBacterialMutationResidues 36-572 (see comments)PromoterT7Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CamKII-GFP-P2A-bReaches-minWPRE
Plasmid#118276PurposeExpresses EGFP and bReaChES under a minimal CamKII PromoterDepositorInsertsEGFP
bReaCHES
UseAAVTagsP2AExpressionMutationPromoterCamK IIAvailable sinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGAN200
Plasmid#129203PurposeyTRAP sensor of [PSI+], detects aggregation of Sup35DepositorInsertSup35NM (SUP35 Budding Yeast)
UseSynthetic BiologyTagsExpressionYeastMutationN terminal and middle domainsPromoterSUP35Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
ChR2-2A-Halo
Plasmid#22047PurposeExpression of ChR2-GFP and Halo-YFP for bi-directional control of neuronal activityDepositorInsertChR2-GFP-2A-Halo-YFP
UseTagsGFP and YFPExpressionMammalianMutationNote that there is a S147R mut in YFP. This mutat…PromoterCMVAvailable sinceSept. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFH6
Plasmid#86555PurposeSubcloning of any sgRNA via BbsI sites. Note that there is an improved version of this plasmid (Addgene 105866) that results in up to 10x greater efficiencyDepositorInsertU6-26p::sgRNA scaffold
UseCRISPR; SubcloningTagsExpressionMutationPromoterArabidopsis U6-26 promoterAvailable sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE
Plasmid#202199PurposeExpresses bicistronically soma-targeted ChrimsonR in frame with EGFP and the optimized PdCO under control of human synapsin1 promotor after Cre-dependent recombinationDepositorHas ServiceAAV1 and AAV5InsertstChrimsonR, PdCO
UseAAV and Cre/LoxTagsEGFP and Rho1D4ExpressionMammalianMutationPromoterhSyn1Available sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPY22-mNeonGreen-Hyg
Plasmid#137082PurposeCodon-optimized mNeonGreen for expression in Arabidopsis thaliana and Physcomitrella patensDepositorInsertmNeonGreen-Flag
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-NtermRGS2-GFP-CIBN
Plasmid#112258PurposeExpresses alpha-helix component of opto-RGS2 in mammalian cellsDepositorInsertNtermRGS2-GFP-CIBN
UseLentiviralTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUB-Cas9-@GL1
Plasmid#86779PurposeDisruption of GLABROUS1 gene in Arabidopsis using CRISPR/Cas9DepositorInsertsgRNA against GLABROUS1
UseSynthetic BiologyTagsExpressionPlantMutationPromoterArabidopsis U6-26 promoterAvailable sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAGM38869
Plasmid#153220PurposeLevel 0 Arabidopsis U6-promoter vectorDepositorInsertArabidopsis U6 snRNA promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
RhGC(BE)_pGEM
Plasmid#85469PurposeThe rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP signalingDepositorInsertrhodopsin-guanylyl cyclase
UseTagsExpressionMammalianMutationPromoterT7 PromoterAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
SMR3B-bio-His
Plasmid#52183PurposeExpresses full-length Submaxillary gland androgen-regulated protein 3B precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertSMR3B (SMR3B Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 GfaABC1D ChR2(H134R) mCherry SV40
Plasmid#112496PurposeExpresses channel rhodopsin in astrocytesDepositorInsertChR2(H134R)
UseAAVTagsmCherryExpressionMutationH134RPromoterGfaABC1DAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV CaMKIIa ChR2 E90R D156N T159C 2A tDimer
Plasmid#52878PurposeChloride-conducting Channelrhodopsin with slow kinetics, neuron-specific promoter, plus red fluorescent protein, excitatory neuronsDepositorInsertsChannelrhodopsin-2
red fluorescent protein
UseAAVTagsExpressionMammalianMutationE90R, D156N, T159CPromoterCaMKIIaAvailable sinceJuly 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLEELA
Plasmid#79752Purposebinary DEST vector for plant expressionDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoter2xPro35SAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV CaMKIIa iChloC 2A tDimer
Plasmid#66709PurposeChloride-conducting Channelrhodopsin with neuron-specific promoter, plus red fluorescent proteinDepositorInsertsChannelrhodopsin-2
red fluorescent protein
UseAAVTagsExpressionMammalianMutationE83Q,E90R,E101S,D156N,T159CPromoterCaMKIIaAvailable sinceOct. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDGE1108
Plasmid#202772PurposeCRISPR-KO in Arabidopsis thalianaDepositorInsertsFAST marker
pRPS5a:zCas9_ttriple
ccdB cassette (BsaI-excisable)
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDGE1176
Plasmid#202774PurposeCRISPR-KO in Arabidopsis thalianaDepositorInsertsFAST marker
pRPS5a:zCas9_tNbEU
ccdB cassette (BsaI-excisable)
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only