We narrowed to 2,475 results for: T2A

1. psBIG1a ActBT2AmcherryP2AP -TagBFPH

   Plasmid

   #229971

   Purpose

   Harbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains Last ActB exon fused T2A-mCherry-P2A-puro and mTagBFPnls-T2A-Hygro

   Depositor

   Martin Pelosse

   Article

   Pelosse et al Synthetic Biology 2025.02.13

   Insert

   ActBT2AmcherryP2AP -TagBFPH
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. NFfshb-t2a-GFP

   Plasmid

   #194356

   Purpose

   over expression vector for killifish fshb tagged with GFP

   Depositor

   Itamar Harel

   Article

   Moses et al eLife. 2023 Oct 24;12:e85960. doi: 10.75

   Insert

   follicle stimulating hormone beta subunit
   

   Use

   Lentiviral

   Tags

   t2a-GFP

   Expression

   Mammalian

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. NFlhb-t2a-GFP

   Plasmid

   #194357

   Purpose

   over expression vector for killifish lhb tagged with GFP

   Depositor

   Itamar Harel

   Article

   Moses et al Nat Aging. 2024 Jun;4(6):791-813. doi: 1

   Insert

   luteinizing hormone subunit beta
   

   Use

   Lentiviral

   Tags

   t2a-GFP

   Expression

   Mammalian

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. T2A GFP H2B

   Plasmid

   #227722

   Purpose

   Plasmid contains T2A GFP H2B, with some nlp-51 homology sequence before, Amp Resistant

   Depositor

   Haosheng Sun, Oliver Hobert

   Article

   Sun et al MicroPubl Biol. 2023 Nov 2;2023:10.17912

   Insert

   (nlp-51 homology) T2A 3xNLS GFP (nlp-51 Nematode)
   

   Use

   Cloning vector

   Tags

   T2A 3xNLS GFP

   Available Since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGGB-V5-TurboID-MUTT2A-C

   Plasmid

   #218897

   Purpose

   golden gate cloning

   Depositor

   Sven Eyckerman

   Article

   Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

   Insert

   V5-TurboID-MUTT2A
   

   Use

   Synthetic Biology

   Available Since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGGB-V5-TurboID-T2A-C

   Plasmid

   #218896

   Purpose

   golden gate cloning

   Depositor

   Sven Eyckerman

   Article

   Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

   Insert

   V5-TurboID-T2A
   

   Use

   Synthetic Biology

   Available Since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. FSHB-t2a-dTOMATO

   Plasmid

   #205597

   Purpose

   an overexpression vector of the killifish FSHB

   Depositor

   Itamar Harel

   Article

   Moses et al eLife. 2023 Oct 24;12:e85960. doi: 10.75

   Insert

   FSHB
   

   Tags

   dTOMATO

   Promoter

   CMV

   Available Since

   March 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNKX2-5-GFP-T2A

   Plasmid

   #203608

   Purpose

   Donor plasmid to create 5' knockin of NKX2-5 with GFP-T2A

   Depositor

   Matthew Birket

   Article

   Douglas et al Stem Cell Res. 2023 Dec 3;74:103262. doi

   Insert

   NKX2-5-GFP-T2A-NKX2-5 (NKX2-5 Human)
   

   Use

   Human targeting

   Available Since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. WT_IFL_G1A-T2A-A28C_R

   Plasmid

   #200185

   Purpose

   WT PA14 I-F Leader and G1A-T2A-A28C Repeat

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   Wildtype I-F Leader and a mutant I-F Locus (G1A-T2A-A28C)
   

   Expression

   Bacterial

   Mutation

   G1A-T2A-A28C in the I-F repeat

   Available Since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDropIn-T2A-ZpGal4DBD

    Plasmid

    #199199

    Purpose

    Insert to create ZpGal4DBD knock-ins, via Drop-In cloning, released via BsmBI digest

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

    Insert

    T2A-ZpGal4DBD
    

    Expression

    Insect

    Available Since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDropIn-T2A-p65Zp

    Plasmid

    #199200

    Purpose

    Insert to create p65Zp knock-ins, via Drop-In cloning, released via BsmBI digest

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

    Insert

    T2A-p65Zp
    

    Expression

    Insect

    Available Since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKozak_mTagBFP2-T2A-sGFP11x7_synCoTC

    Plasmid

    #199495

    Purpose

    Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

    Depositor

    Robert Bryson-Richardson

    Article

    Miles et al bioRxiv 2023

    Insert

    mTagBFP2-T2A-sGFP11x7
    

    Use

    CRISPR

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSA_0_mTagBFP2-T2A-sGFP11x7_synCoTC

    Plasmid

    #199478

    Purpose

    Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

    Depositor

    Robert Bryson-Richardson

    Article

    Miles et al bioRxiv 2023

    Insert

    mTagBFP2-T2A-sGFP11x7
    

    Use

    CRISPR

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSA_1_mTagBFP2-T2A-sGFP11x7_synCoTC

    Plasmid

    #199479

    Purpose

    Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

    Depositor

    Robert Bryson-Richardson

    Article

    Miles et al bioRxiv 2023

    Insert

    mTagBFP2-T2A-sGFP11x7
    

    Use

    CRISPR

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSA_2_mTagBFP2-T2A-sGFP11x7_synCoTC

    Plasmid

    #199480

    Purpose

    Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

    Depositor

    Robert Bryson-Richardson

    Article

    Miles et al bioRxiv 2023

    Insert

    mTagBFP2-T2A-sGFP11x7
    

    Use

    CRISPR

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV_tKiMBImut-T2A-caMEK

    Plasmid

    #199579

    Purpose

    Express tKiMBImut(AA) and caMEK in an AAV vector

    Depositor

    Michael Lin

    Article

    Wu et al ACS Cent Sci. 2023 Mar 20;9(4):719-732.

    Inserts

    ERK tdTomato-Kinase-Modulated Bioluminescent Indicator (mutant)
    constitutively active MEK
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. LFNG:GFPnls-T2a-ER-Cre-ER

    Plasmid

    #200107

    Depositor

    Prayag Murawala

    Insert

    LFNG:GFPnls-T2A-ER-Cre-ER
    

    Use

    Unspecified

    Available Since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. IR76b-T2A-QF2

    Plasmid

    #162523

    Purpose

    Plasmid for CRISPR-mediated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment replacing the stop codon of the endogenous Aedes aegypti Ir76b gene

    Depositor

    Leslie Vosshall

    Article

    Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.

    Insert

    Ir76b-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir76b-right-HDR-arm
    

    Expression

    Insect

    Available Since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. GR3-T2A-QF2

    Plasmid

    #162521

    Purpose

    Plasmid for CRISPR-mediated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment replacing the stop codon of the endogenous Aedes aegypti Gr3 gene

    Depositor

    Leslie Vosshall

    Article

    Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.

    Insert

    Gr3-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Gr3-right-HDR-arm
    

    Expression

    Insect

    Available Since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. IR8a-T2A-QF2

    Plasmid

    #162520

    Purpose

    Plasmid for CRISPR-mediated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment replacing the stop codon of the endogenous Aedes aegypti Ir8a gene

    Depositor

    Leslie Vosshall

    Article

    Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.

    Insert

    Ir8a-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir8a-right-HDR-arm
    

    Expression

    Insect

    Available Since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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