Addgene

We narrowed to 304 results for: Tpr

Showing: 221 - 240 of 304 results

Results per page:

Sort results by:

pTorPE-jREX-GECO1

Plasmid

#113941

Purpose

Bacterial expression of red fluorescent Ca2+ sensor jREX-GECO1

Depositor

Robert Campbell, Thomas Hughes

Article

Molina et al Biophys J. 2019 May 21;116(10):1873-1886

Insert

jREX-GECO1

Use

Tags

6xHis and Modified TorA MNNNDLFQASRRRFLAQLG[G to …

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pETM6-HpaBC

Plasmid

#71260

Purpose

Expresses E. coli HpaBC in monocistronic form from ePathBrick vector, pETM6

Depositor

Mattheos Koffas

Article

Jones et al Biotechnol Prog. 2015 Oct 21. doi: 10.10

Inserts

HpaB
HpaC

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 4, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-R4-uAb

Plasmid

#101800

Purpose

Mammalian cell expression of the beta-galactosidase-specific ubiquibody, R4-uAb

Depositor

Matthew DeLisa

Article

Portnoff et al J Biol Chem. 2014 Mar 14;289(11):7844-55

Insert

R4-uAb

Use

Tags

6xHis and Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTorPE-G-GECO1

Plasmid

#32466

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

G-GECO1.0

Use

Lab constructed

Tags

6 His tag and Modified TorA MNNNDLFQASRRRFQAQLGGL…

Expression

Bacterial

Mutation

GCaMP3 K119I/L173Q/S404G/E430V

Promoter

Available since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-link-GFPγ-SpHis5

Plasmid

#60781

Purpose

Yeast C-terminal tagging vector, which creates gene fusions with GFPγ using SpHis5 selection

Depositor

Linda Huang

Article

Slubowski et al Yeast. 2015 Jan 22. doi: 10.1002/yea.306

Insert

GFPγ

Use

Yeast integrating

Tags

Expression

Mutation

T65C S72A V163A I167T L231H

Promoter

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pTorPE-G-GECO1.1

Plasmid

#32467

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

G-GECO1.1

Use

Lab constructed

Tags

6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…

Expression

Bacterial

Mutation

GCaMP3 K69E/N77Y/D86G/K119I/L173Q/K380N/S404G/E43…

Promoter

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMK83

Plasmid

#226793

Purpose

Integrates slr0214 (HIP1 methyltransferase) into gidB-atpI locus of E. coli DH10B

Depositor

David Nielsen

Article

Kamoku et al Biotechnol Prog. 2025 Jan 23:e3518. doi:

Insert

slr0214 (slr0214 Synechocystis sp. PCC 6803)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Tetracycline resistance cassette promoter (consti…

Available since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGM209

Plasmid

#220174

Purpose

Expresses exogenous wild-type TTC1

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

TTC1 (TTC1 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-Myc-Caspase-11_humanized-mutant

Plasmid

#214316

Purpose

Expression of gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (Casp4 Mouse)

Use

Retroviral

Tags

Myc

Expression

Mutation

KLS288YKT_KASIHS348TPRAKA

Promoter

MESV

Available since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDF-Leup

Plasmid

#213154

Purpose

Expresses the leupeptin protease inhibitor pathway for bolstered production of heterologously expressed proteins.

Depositor

Jason Crawford

Article

Li et al J Nat Prod. 2021 Oct 22;84(10):2692-2699

Insert

Leupeptin Protease Inhibitor Pathway

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX6P1-hPREP

Plasmid

#179387

Purpose

Express human prolyl oligopeptidase (tagged) in bacterial cells

Depositor

Christian Gruber

Article

Retzl et al J Nat Prod. 2020 Nov 25;83(11):3305-3314

Insert

human prolyl oligopeptidase (PREP Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

TAC

Available since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBOB-CARMIL2 BH domain-GFP

Plasmid

#118736

Purpose

To express CARMIL2 BH tagged with GFP on C-terminus

Depositor

John Cooper

Article

Lanier et al J Biol Chem. 2016 Jan 15;291(3):1076-91.

Insert

CARMIL2 BH domain (CARMIL2 Human)

Use

Lentiviral

Tags

GFP

Expression

Mutation

Promoter

CMV

Available since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-link-GFPEnvy-SpHis5

Plasmid

#60782

Purpose

Yeast C-terminal tagging vector, which creates gene fusions with GFPEnvy using SpHis5 selection

Depositor

Linda Huang

Article

Slubowski et al Yeast. 2015 Jan 22. doi: 10.1002/yea.306

Insert

GFPEnvy

Use

Yeast integrating

Tags

Expression

Mutation

S30R Y39N T65C S72A F99S N105T Y145F M153T V163A …

Promoter

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
mEos3.2trCD45

Plasmid

#203753

Purpose

Encodes the transmembrane domain from CD45 with mEos3.2 fused on the N terminus. Used as a fluorescent plasma membrane probe.

Depositor

Sarah Veatch

Article

Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

Insert

trCD45 (PTPRC Human)

Use

Tags

mEos3.2

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
CD45 sp-SNAPf-CD45R0Ex-His10 (XSB316)

Plasmid

#78509

Purpose

Construct for expressing CD45R0 (extracellular domain) His10 in Sf9

Depositor

Ron Vale

Article

Su et al Science. 2016 Apr 29;352(6285):595-9. do

Insert

CD45R0Ex (PTPRC Human)

Use

Tags

His10 and SNAPf

Expression

Insect

Mutation

Promoter

Available since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac (GST-preSci site-CD45)

Plasmid

#177889

Purpose

Baculo expression of GST tag fused to human CD45 ICD

Depositor

Ron Vale

Article

Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

Insert

CD45 (aa 598-1304 only) (PTPRC Human)

Use

Tags

TEV-GST-PreScission cut site

Expression

Insect

Mutation

L652P

Promoter

Polyhedrin

Available since

Aug. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBOB-CARMIL2 BH domain-tdTomato

Plasmid

#118737

Purpose

To express CARMIL2 tagged BH with tdTomato on C-terminus

Depositor

John Cooper

Article

Lanier et al J Biol Chem. 2016 Jan 15;291(3):1076-91.

Insert

CARMIL2 BH domain (CARMIL2 Human)

Use

Lentiviral

Tags

tdTomato

Expression

Mutation

Promoter

CMV

Available since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTorPE-G-GECO1.2

Plasmid

#32468

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

G-GECO1.2

Use

Lab constructed

Tags

6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…

Expression

Bacterial

Mutation

GCaMP3 K69E/N77Y/D86G/K119I/L173Q/D260G/S404G/E43…

Promoter

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pTorPE-B-GECO1

Plasmid

#32469

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

B-GECO1.0

Use

Lab constructed

Tags

6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…

Expression

Bacterial

Mutation

GCaMP3 S40P/L60P/D86G/K119I/L173Q/T223S/Y224H/D30…

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-link-GFPIvy-SpHis5

Plasmid

#60783

Purpose

Yeast C-terminal tagging vector, which creates gene fusions with GFPIvy using SpHis5 selection

Depositor

Linda Huang

Article

Slubowski et al Yeast. 2015 Jan 22. doi: 10.1002/yea.306

Insert

GFPIvy

Use

Yeast integrating

Tags

Expression

Mutation

S30R Y39N T65C Q69A S72A F99S N105T Y145F M153T V…

Promoter

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge