We narrowed to 13,735 results for: TIM;

1. pCMV-T7-SpG-P2A-mScarlet (MNW006)

   Plasmid

   #174136

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized SpG with BPNLS-3xFLAG-P2A-mScarlet
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-mScarlet

   Expression

   Mammalian

   Mutation

   SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

   Promoter

   CMV and T7

   Available since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYES2.1-pomMet17

   Plasmid

   #107453

   Purpose

   Overexpression of MET fusion protein in S. cerevisiae

   Depositor

   Clare O'Connor

   Article

   Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

   Insert

   Met17 (met17 Fission Yeast)
   

   Use

   Tags

   V5-His

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMOS031: superecliptic pHluorin (endoplasmic reticulum)

   Plasmid

   #163051

   Purpose

   superecliptic pHluorin (endoplasmic reticulum)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   superecliptic pHluorin
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFA6a-link-yoLSS-mKate2-SpHis5

   Plasmid

   #44848

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized LSS-mKate2 with SpHis5 selection

   Depositor

   Wendell Lim, Kurt Thorn

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yoLSS-mKate2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. csr-1_son-1_mApple

   Plasmid

   #191741

   Purpose

   Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

   Depositor

   Jay C Dunlap

   Article

   Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

   Insert

   NCU04288 (NCU04288 Neurospora crassa)
   

   Use

   Tags

   NCU04502 promoter and mApple

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. csr-1_son-1_mCherry

   Plasmid

   #191742

   Purpose

   Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

   Depositor

   Jay C Dunlap

   Article

   Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

   Insert

   NCU04288 (NCU04288 Neurospora crassa)
   

   Use

   Tags

   NCU04502 promoter and mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. tubulin-CeNL_pcDNA3

   Plasmid

   #89515

   Purpose

   Cyan color variant of bright luminescent protein, fused to b-tubulin

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   CeNL
   

   Use

   Tags

   b-tubulin

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. lentiCRISPRv2-7SK-Blast

   Plasmid

   #183620

   Purpose

   lentiCRISPRv2 derivative with modified 7SK promoter driving sgRNA and encoding Blasticidin resistance. Compatible with inserts designed for lentiCRISPRv2.

   Depositor

   Bill Sugden

   Article

   Hutcheson et al ACS Synth Biol. 2022 Jun 29. doi: 10.102

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Modified 7SK

   Available since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHT-trxA.ads

   Plasmid

   #47383

   Purpose

   Expresses ads with trxA tag under Pgrac promoter (inducible by IPTG) in bacteria

   Depositor

   Heng-Phon Too

   Article

   Zhou et al Biotechnol Bioeng. 2013 Mar 8. doi: 10.1

   Insert

   ads
   

   Use

   Tags

   6xHis and trxA

   Expression

   Bacterial

   Mutation

   Promoter

   Pgrac

   Available since

   Oct. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. BCJJ340

    Plasmid

    #117502

    Purpose

    UBI10:Cas9:E9 Golden Gate Level 1 Position 2

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BpiI:GCAA:UBI10:Cas9-3(intron):E9:ACTA:BpiI
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtUBI10

    Available since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSUPER-retro-puro-shNup133-1

    Plasmid

    #87334

    Purpose

    To express shRNA against human Nup133

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    shRNA against human Nup133
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCIS.MTRIA-PRLH

    Plasmid

    #184618

    Purpose

    For expression of a fluorescent sensor for Prolactin-releasing peptide factor in mammalian cells

    Depositor

    Hiroshi Hibino, Daisuke Ino

    Article

    Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

    Insert

    MTRIA-PRLH
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCIS.MTRIA-NOC

    Plasmid

    #184611

    Purpose

    For expression of a fluorescent sensor for nociceptin in mammalian cells

    Depositor

    Hiroshi Hibino, Daisuke Ino

    Article

    Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

    Insert

    MTRIA-NOC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    June 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. YN2_1_LT84_RPL13A

    Plasmid

    #177712

    Purpose

    Expresses Cas9 and sgRNA cassette for targeting RPL13A in yeast Saccharomyces cerevisiae

    Depositor

    Lisbeth Olsson

    Article

    Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. YeNL-Actin_pcDNA3

    Plasmid

    #89521

    Purpose

    Yellow color variant of bright luminescent protein, fused to actin

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    YeNL
    

    Use

    Tags

    Actin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNB777

    Plasmid

    #60153

    Purpose

    Red click beetle luciferase (Promega) driven by LEU1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    CBR
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    LEU1pr

    Available since

    Nov. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR-L4-pTF-L1R

    Plasmid

    #45954

    Depositor

    Patrick Salmon

    Article

    Giry-Laterriere et al Hum Gene Ther. 2011 Oct;22(10):1255-67.

    Insert

    pTF promoter
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    TET optimized

    Available since

    Aug. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pME-QF2w-SV40pA

    Plasmid

    #155117

    Purpose

    Middle entry vector containing QF2w

    Depositor

    Bret Pearson

    Article

    Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

    Insert

    QF2w
    

    Use

    Gateway middle entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. KA2897_pPB-TREtight-UBCmin-cHA-IRESH2BmKO2

    Plasmid

    #124176

    Purpose

    piggyBac-based Dox-inducible expression (moderate)

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. tubulin-YeNL_pcDNA3

    Plasmid

    #89519

    Purpose

    Yellow color variant of bright luminescent protein, fused to b-tubulin

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    YeNL
    

    Use

    Tags

    b-tubulin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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