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We narrowed to 15,942 results for: LEA

Showing: 5951 - 6000 of 15942 results

  14. pCAGGS-Twin-strep-TEV-DGKδ2-DSAMD

    Plasmid
    #223721
    Purpose
    Express human diacylglycerol kinase delta 2- SAM domain deletion mutant (N-terminal TEV protease cleavable Twin-Strep-fusion protein) in mammalian cell
    Depositor
    Chiaki Murakami
    Insert
    DGKD (DGKD Human)
    Use
    Tags
    TEV cleavable site and Twin-Strep-tag
    Expression
    Mammalian
    Mutation
    deleted sterile alpha motif domain (deleted amino…
    Promoter
    CAG and chicken β-actin promoter
    Available Since
    Jan. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    223721-plasmid-map-sequence-id-457432

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  18. SpCas9-miniCGBE1

    Plasmid
    #198548
    Purpose
    Expresses human codon-optimized SpCas9-miniCGBE1 and blasticidin resistance: EFS promoter-SpCas9-miniCGBE1-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-miniCGBE1
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available Since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198548-plasmid-map-sequence-id-388891

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  19. SpCas9-NG-miniCGBE1

    Plasmid
    #198538
    Purpose
    Expresses human codon-optimized SpCas9-NG-miniCGBE1 and blasticidin resistance: EFS promoter-SpCas9-NG-miniCGBE1-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NG-miniCGBE1
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198538-plasmid-map-sequence-id-388888

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  20. SpCas9-NRRH

    Plasmid
    #198540
    Purpose
    Expresses human codon-optimized SpCas9-NRRH and blasticidin resistance: EFS promoter-SpCas9-NRRH-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NRRH
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available Since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198540-plasmid-map-sequence-id-388897

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  21. SpCas9-NRCH

    Plasmid
    #198542
    Purpose
    Expresses human codon-optimized SpCas9-NRCH and blasticidin resistance: EFS promoter-SpCas9-NRCH-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NRCH
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available Since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198542-plasmid-map-sequence-id-388906

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  25. pACB143

    Plasmid
    #222214
    Purpose
    For recombinant expression of vanB (PP_3737 from Pseudomonas putida) with the A24P mutation and an N-terminal thrombin-cleavable His tag
    Depositor
    Gregg Beckham
    Article
    Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.
    Insert
    vanB(A24P)
    Use
    Tags
    N-terminal, thrombin-cleavable 6x His tag
    Expression
    Bacterial
    Mutation
    Changed Alanine 24 to Proline
    Promoter
    T7
    Available Since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222214-plasmid-map-sequence-id-438176

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  26. pETSUMO2_Ideonella-bGSDM-WELQ

    Plasmid
    #222098
    Purpose
    For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Ideonella species with a WELQut protease cleavage site for controlled activation.
    Depositor
    Philip Kranzusch
    Article
    Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:
    Insert
    Ideonella-bGSDM-WELQ ORF
    Use
    Tags
    6xHis-SUMO2
    Expression
    Bacterial
    Mutation
    N-terminal methionine deleted and replaced by sin…
    Promoter
    T7
    Available Since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222098-plasmid-map-sequence-id-437096

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  27. pcDNA5-FRT/T0-3XFLAG-NESm-NFATC2IP

    Plasmid
    #221445
    Purpose
    Mammalian expression vector of triple Flag tagged NFATC2IP ORF with mutant nuclear export signal (NESm) at N-terminus
    Depositor
    Daniel Durocher
    Article
    Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.
    Insert
    NFATC2IP (NFATC2IP Human)
    Use
    Tags
    3XFlag and Mutant Nuclear Export Signal (mNES)
    Expression
    Mammalian
    Mutation
    mutant NES (NESm: NLVDLQKKLEEAKADEQQ (PMID: 10739…
    Promoter
    CMV
    Available Since
    July 3, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    221445-plasmid-map-sequence-id-437442

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  28. pILGFPEasy9R

    Plasmid
    #218291
    Purpose
    Plasmid-free in-situ promoter cloning and characterisation system in S. cerevisiae, allows the characterisation of a bidirectional promoter using yEGFP and E2-Crimson as reporters.
    Depositor
    Claudia Vickers
    Article
    McDonnell et al bioRxiv 2024.03.26.586389
    Insert
    pURA3>KlURA3>tKlURA3-tPGK1KanMX>tAgTEF-pMAL32>MazF(RPL28i)-E2Crimson>tPDC1-pDDI2>ISceI>tSynth3>ura3(4,191)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218291-plasmid-map-sequence-id-433736

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  30. TDP-43 mRuby K84ONBK

    Plasmid
    #216226
    Purpose
    TDP-43 with a C-terminal mRuby tag and Lysine 84 in the NLS mutated to a TAG stop codon for amber codon suppression.
    Depositor
    Jennifer Lee
    Article
    Shadish et al Biophys Chem. 2024 Jan 23;307:107191. do
    Insert
    Transactive response DNA binding protein of 43 kDa (TARDBP Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Lysine 84 mutated to a TAG stop codon
    Promoter
    CMV Promoter
    Available Since
    April 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    216226-plasmid-map-sequence-id-431056

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  31. pCL1318

    Plasmid
    #217960
    Purpose
    Expression of human H4 carrying three point mutations to disrupt binding with H3. Expression in mammalian cells driven by the CMV promoter.
    Depositor
    Andrew Bowman
    Article
    Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755
    Insert
    Histone H4 (H4C1 Human)
    Use
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    L63A, F66A, I71A
    Promoter
    CMV
    Available Since
    April 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    217960-plasmid-map-sequence-id-430214

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  32. pCL1317

    Plasmid
    #217959
    Purpose
    Expression of human H4 carrying a triple glycine insertion to disrupt folding with H3. Expression in mammalian cells driven by the CMV promoter.
    Depositor
    Andrew Bowman
    Article
    Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755
    Insert
    Histone H4 (H4C1 Human)
    Use
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    GGG insertion between V65 and F66
    Promoter
    CMV
    Available Since
    April 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    217959-plasmid-map-sequence-id-430213

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  33. pCL1315

    Plasmid
    #217957
    Purpose
    Expression of human H3.1 carrying a triple point mutation to disrupt folding with histone H4. Expression in mammalian cells driven by the CMV promoter.
    Depositor
    Andrew Bowman
    Article
    Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755
    Insert
    Histone H3.1 (H3C1 Human)
    Use
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    F84A, L92A, Y99A
    Promoter
    CMV
    Available Since
    April 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    217957-plasmid-map-sequence-id-430211

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  34. pCL1314

    Plasmid
    #217956
    Purpose
    Expression of human H3.1 carrying a triple glycine insertion to disrupt folding with H4. Expression in mammalian cells driven by the CMV promoter.
    Depositor
    Andrew Bowman
    Article
    Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755
    Insert
    Histone H3.1 (H3C1 Human)
    Use
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    GGG insertion between A95 and C96
    Promoter
    CMV
    Available Since
    April 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    217956-plasmid-map-sequence-id-430210

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  35. pMAT_M4_YVTM_YQNQ

    Plasmid
    #197084
    Purpose
    This plasmid contains the coding sequence for signaling peptide M4, which contains the YVTM and YQNQ signaling motifs. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP
    Depositor
    Wendell Lim
    Article
    Daniels et al Science. 2022 Dec 16;378(6625):1194-1200
    Insert
    This plasmid contains the coding sequence for signaling peptide M4, which contains the YVTM and YQNQ signaling motifs.
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    April 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    197084-plasmid-map-sequence-id-386821

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  36. pMAT_M10_PQQAT

    Plasmid
    #197093
    Purpose
    This plasmid contains the coding sequence for signaling peptide M10, which contains the PQQAT signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP
    Depositor
    Wendell Lim
    Article
    Daniels et al Science. 2022 Dec 16;378(6625):1194-1200
    Insert
    This plasmid contains the coding sequence for signaling peptide M10, which contains the PQQAT signaling motif.
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    April 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    197093-plasmid-map-sequence-id-386838

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  37. pMAT_M11_PQEINF

    Plasmid
    #197094
    Purpose
    This plasmid contains the coding sequence for signaling peptide M11, which contains the PQEINF signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP
    Depositor
    Wendell Lim
    Article
    Daniels et al Science. 2022 Dec 16;378(6625):1194-1200
    Insert
    This plasmid contains the coding sequence for signaling peptide M11, which contains the PQEINF signaling motif.
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    April 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    197094-plasmid-map-sequence-id-386825

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  38. pMAT_M12_RPTAVEG

    Plasmid
    #197095
    Purpose
    This plasmid contains the coding sequence for signaling peptide M12, which contains the RPTAVEG signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP
    Depositor
    Wendell Lim
    Article
    Daniels et al Science. 2022 Dec 16;378(6625):1194-1200
    Insert
    This plasmid contains the coding sequence for signaling peptide M12, which contains the RPTAVEG signaling motif.
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    April 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    197095-plasmid-map-sequence-id-386819

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  39. pMAT_M13_6xSAG

    Plasmid
    #197096
    Purpose
    This plasmid contains the coding sequence for the control peptide M13, which contains a linker with six repeats of serine-alanine-glycine. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_C
    Depositor
    Wendell Lim
    Article
    Daniels et al Science. 2022 Dec 16;378(6625):1194-1200
    Insert
    Control peptide M13, which contains a linker with six repeats of serine-alanine-glycine (SAG)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    April 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    197096-plasmid-map-sequence-id-386824

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  41. pCF702

    Plasmid
    #209650
    Purpose
    Suicide plasmid carrying a neomycin resistance marker flanked by homologous regions of the Mth60-fimbria encoding operon of Methanothermobacter thermautotrophicus
    Depositor
    Bastian Molitor
    Article
    Fink et al Appl Environ Microbiol. 2023 Jul 26;89(7
    Inserts
    Downstream homologous region
    thermostable neomycin phosphotransferase gene
    Upstream homologous region
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    March 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    209650-plasmid-map-sequence-id-416212

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Showing: 5951 - 6000 of 15942 results