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pCMV_N7-BE4-puro

Plasmid

#226584

Purpose

Express N7-BE4 base editor in mammalian cells

Depositor

Gang Wang

Article

Zhao et al Nat Commun. 2024 Sep 17;15(1):8140. doi:

Insert

N7-BE4

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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Lenti-E2-ABE-Cas9n-puro

Plasmid

#226587

Purpose

Express E2-ABE in mammalian cells

Depositor

Gang Wang

Article

Zhao et al Nat Commun. 2024 Sep 17;15(1):8140. doi:

Insert

E2-ABE

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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TC1332

Plasmid

#164874

Purpose

CURE-N: C to U RNA Editing, with high efficiency and low off-target effects

Depositor

Tian Chi

Article

Huang et al EMBO J. 2020 Nov 16;39(22):e104741. doi:

Insert

hAPOBEC3A fused to PspCas13b (APOBEC3A )

Use

CRISPR

Expression

Mammalian

Mutation

human apobec Y132D

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pJJGL001

Plasmid

#180605

Purpose

Plasmid expressing E. coli codon optimized His-MBP-TEV-DpbCasX

Depositor

Jennifer Doudna

Article

Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

Insert

DpbCasX

Use

CRISPR

Tags

10x His and MBP

Expression

Bacterial

Promoter

T7

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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TC1681

Plasmid

#164872

Purpose

CURE-C2: C to U RNA Editing, with high efficiency and high off-target effects

Depositor

Tian Chi

Article

Huang et al EMBO J. 2020 Nov 16;39(22):e104741. doi:

Insert

hAPOBEC3A fused to PspCas13b (APOBEC3A )

Use

CRISPR

Expression

Mammalian

Mutation

human apobec Y132D

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pINDUCER21-HOXA9

Plasmid

#51302

Purpose

Expression of HOXA9 after doxycycline administration

Depositor

George Daley

Article

Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

Insert

homeobox A9 (HOXA9 Human)

Use

Lentiviral

Tags

n/a

Mutation

n/a

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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HA-FRMD6

Plasmid

#32838

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

FRMD6 (FRMD6 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 15, 2011

Availability

Academic Institutions and Nonprofits only
Sa-SlugCas9

Plasmid

#163797

Purpose

Expresses Sa-SlugCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET28b-GBP-MTn

Plasmid

#154363

Purpose

Bacterial expression of MTn

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

MTn

Tags

His

Expression

Bacterial

Available Since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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TC1614

Plasmid

#164873

Purpose

CURE-X: C to U RNA Editing, with low off-target effects but also lower efficiency

Depositor

Tian Chi

Article

Huang et al EMBO J. 2020 Nov 16;39(22):e104741. doi:

Insert

hAPOBEC3A inserted into RfxCas13d (APOBEC3A )

Use

CRISPR

Expression

Mammalian

Mutation

human apobec Y132D

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pMAL-C2X dAmp/Kana+

Plasmid

#154352

Purpose

Empty backbone vector

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Type

Empty backbone

Expression

Bacterial

Available Since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCDFDuet-AtUBC8-S-AtUBA1-S

Plasmid

#222714

Purpose

Expression AtUBA1-S and AtUBC8-S in in bacterial cells

Depositor

Lei Wu

Article

Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

Inserts

AtUBC8 (UBC8 Mustard Weed)
ATUBA1 (UBA1 Mustard Weed)

Tags

S-Tag

Expression

Bacterial

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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Cas12j-8-puro

Plasmid

#194966

Purpose

The plasmid expresses human codon-optimized Cas12j-8 and puromycin resistence gene.

Depositor

Yongming Wang

Article

Wang et al Sci Adv. 2023 Feb 10;9(6):eabo6405. doi:

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAVf-EnhCB-lacZnls miR-122 3x

Plasmid

#35644

Depositor

Phillip Zamore

Article

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Insert

3 miR-122 target sites

Use

AAV

Promoter

CB

Available Since

April 25, 2012

Availability

Academic Institutions and Nonprofits only

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RV-Cag-Dio-EYFP-P2A-Mafb

Plasmid

#98175

Purpose

Retroviral vector encoding Cre-dependent expression of EYFP-P2A-Mafb

Depositor

Oscar Marin

Article

Mi et al Science. 2018 Feb 22. pii: science.aar68

Insert

Mafb (Mafb Mouse)

Use

Retroviral

Promoter

CAG

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pJJGL002

Plasmid

#180606

Purpose

Plasmid expressing E. coli codon optimized His-MBP-TEV-PlmCasX

Depositor

Jennifer Doudna

Article

Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

Insert

PlmCasX

Use

CRISPR

Tags

10xHis and MBP

Expression

Bacterial

Promoter

T7

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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RV-Cag-Dio-EYFP-P2A-cMaf

Plasmid

#98174

Purpose

Retroviral vector encoding Cre-dependent expression of EYFP-P2A-Maf

Depositor

Oscar Marin

Article

Mi et al Science. 2018 Feb 22. pii: science.aar68

Insert

cMaf (codon optimzed) (Maf Mouse)

Use

Retroviral

Mutation

Codon optimized

Promoter

CAG

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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pIND-hygro-HA-H3.3

Plasmid

#47981

Purpose

Ponasterone A-inducible expression of human HA tagged H3F3A

Depositor

Bing Zhu

Article

Huang et al PLoS Genet. 2013 Jun;9(6):e1003558. doi:

Insert

H3F3A (H3-3A Human)

Tags

HA

Expression

Mammalian

Mutation

288 bp T to C synonymous mutation

Promoter

Minimal Heat Shock Promoter

Available Since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-Ost4-GFP-MTn

Plasmid

#154351

Purpose

Mammalian expression of MTn

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

MTn

Tags

GFP

Expression

Mammalian

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pRNA-U6-let7 sponge

Plasmid

#35664

Depositor

Phillip Zamore

Article

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Insert

U6 let-7sponge

Promoter

U6

Available Since

June 22, 2012

Availability

Academic Institutions and Nonprofits only

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