We narrowed to 31,655 results for: LIS;

1. pKLV2-EF1a-Cas9Bsd-W

   Plasmid

   #68343

   Purpose

   Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the C-terminus

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   EF1a-Cas9 cassette, WPRE
   

   Use

   CRISPR and Lentiviral

   Tags

   Cas9 fused with Flag-tag and a NLS at the N termi…

   Promoter

   EF1a

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-YFP-KRAS4B

   Plasmid

   #112718

   Purpose

   Expresses YFP-KRAS4B fusion protein used for FRET studies in eukaryotic cells.

   Depositor

   Kenneth Westover

   Article

   Ambrogio et al Cell. 2018 Feb 8;172(4):857-868.e15. doi

   Insert

   YFP-KRAS4B (KRAS Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKLV2.2-mU6gRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W

   Plasmid

   #72667

   Purpose

   Lentiviral dual CRISPR gRNA expression vector (mouse U6 and human U6 promoters)

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   mU6gRNA cassette, hU6gRNA cassette, PGKpuroBFP cassette, WPRE
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKLV2-U6gRNA5(gBFP)-PGKmCherry2ABFP-W

   Plasmid

   #67986

   Purpose

   Cas9 activity reporter with mCherry and BFP

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Inserts

   U6gRNA cassette, PGKmCherryABFP cassette, WPRE
   Guide RNA targeting modified BFP
   

   Use

   CRISPR and Lentiviral

   Mutation

   Deleted BbsI site within WPRE, modified BFP

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKLV2-U6gRNA5(BbsI)-PGKpuro2AmAG-W

   Plasmid

   #67976

   Purpose

   CRISPR gRNA expression vector with an improved scaffold and puro/mAG markers

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKpuro2AmAG cassette, WPRE
   

   Use

   Lentiviral

   Mutation

   Deleted BbsI site within WPRE

   Promoter

   human U6 promoter, mouse PGK promoter

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP

   Plasmid

   #67991

   Purpose

   CRISPR gRNA expression vector with an improved l scaffold and puro/BFP markers without WPRE

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette with an improved scaffold, PGKpuro2ABFP cassette
   

   Use

   CRISPR and Lentiviral

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. CAG-LoxpStopLoxp-NLS-dCas9-HA-NLS-NLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-T2A-EGFP-WPRE-PolyA

   Plasmid

   #107307

   Purpose

   Encoding an SPH activation system

   Depositor

   Hui Yang

   Article

   Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/

   Insert

   CAG-LSL-dCas9-10xGCN4-P2A-scFv-P65-HSF1-T2A-EGFP-WPRE-PolyA
   

   Expression

   Mammalian

   Available Since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKLV2-U6gRNA5(Empty)-PGKmCherry2ABFP-W

   Plasmid

   #67985

   Purpose

   Cas9 activity reporter (control) with mCherry and BFP

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKmCherryABFP cassette, WPRE
   

   Use

   CRISPR and Lentiviral

   Mutation

   Deleted BbsI site within WPRE, modified BFP

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGmCherry-gRNA

   Plasmid

   #87110

   Purpose

   gRNA cloning vector with CAGmCherry

   Depositor

   Juan Belmonte

   Article

   Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pC0043-PspCas13b-gRNA LINE1

    Plasmid

    #223699

    Purpose

    gRNA of dPspCas13b-FTO targeting LINE1

    Depositor

    Chuan He

    Article

    Wei et al Science. 2022 May 27;376(6596):968-973.

    Insert

    gRNA target sequence for LINE1
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKLV2-U6gRNA5(BbsI)-PGKpuro2AZsG-W

    Plasmid

    #67975

    Purpose

    CRISPR gRNA expression vector with an improved scaffold and puro/ZsG markers

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKpuro2AZsG cassette, WPRE
    

    Use

    Lentiviral

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    human U6 promoter, mouse PGK promoter

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKLV2-U6gRNA5(gBFP)-PGKGFP2ABFP-W

    Plasmid

    #67984

    Purpose

    Cas9 activity reporter with GFP and BFP

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Inserts

    U6gRNA cassette, PGKGFP2ABFP cassette, WPRE
    Guide RNA targeting modified BFP
    

    Use

    CRISPR and Lentiviral

    Mutation

    Deleted BbsI site within WPRE, modified BFP

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKLV2-U6gRNA3(BbsI)-PGKpuro2ABFP

    Plasmid

    #67990

    Purpose

    CRISPR gRNA expression vector with the conventional scaffold and puro/BFP markers without WPRE

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette with the conventional scaffold, PGKpuro2ABFP cassette
    

    Use

    CRISPR and Lentiviral

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1-CFP-KRAS4B

    Plasmid

    #112717

    Purpose

    Expresses CFP-KRAS4B fusion protein used for FRET studies in mammalian cells.

    Depositor

    Kenneth Westover

    Article

    Ambrogio et al Cell. 2018 Feb 8;172(4):857-868.e15. doi

    Insert

    CFP-KRAS4B (KRAS Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKLV2-U6gRNA5(Empty)-PGKGFP2ABFP-W

    Plasmid

    #67983

    Purpose

    Cas9 activity reporter (control) with GFP and BFP

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKGFP2ABFP cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Mutation

    Deleted BbsI site within WPRE, modified BFP

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Pet22b-mEGFP-YAP-N-IDR

    Plasmid

    #166440

    Purpose

    Bacterial expression of 6x His tagged mEGFP-YAP1-N-IDR fusion protein

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    mEGFP, YAP-N-IDR(1-164) (YAP1 Human)
    

    Expression

    Bacterial

    Available Since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRV-RB

    Plasmid

    #106374

    Purpose

    creating stable line with RFP and BSD resistance

    Depositor

    Tong-Chuan He

    Article

    Liao et al Gene Ther. 2017 Jun;24(6):333-341. doi:

    Insert

    mRFP and blasticidin resistance gene
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Feb. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRV-GN

    Plasmid

    #106373

    Purpose

    creating stable line with GFP and G418 resistance

    Depositor

    Tong-Chuan He

    Article

    Liao et al Gene Ther. 2017 Jun;24(6):333-341. doi:

    Insert

    eGFP and geneticin/G418 resistance gene
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Feb. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Pet22b-mCherry-TEAD4-IDR

    Plasmid

    #166442

    Purpose

    Bacterial expression of 6x His tagged mCherry-TEAD4 -IDR fusion protein

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    mCherry, TEAD4-IDR (TEAD4 Human)
    

    Expression

    Bacterial

    Available Since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Pet22b-mEGFP-YAP-C-IDR

    Plasmid

    #166441

    Purpose

    Bacterial expression of 6x His tagged mEGFP-YAP1-C-IDR fusion protein

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    mEGFP, YAP-C-IDR(266-504) (YAP1 Human)
    

    Expression

    Bacterial

    Available Since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge