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pB-TetOn-DEST-hEF1a-mODC-rtTA-IRES-NEO (JDW 1130)

Plasmid

#229839

Purpose

A CAGGS driven, piggybac compatible, tet-on expression vector containing a luciferase reporter followed by a P2A cleavage peptide and H2A fused mCherry for nuclear labeling.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Luciferase

Expression

Mammalian

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-FLAG-IRES-blasticidin-Sox2 WT

Plasmid

#182046

Purpose

expression of Sox2

Depositor

Hyonchol Jang

Article

Kim et al Exp Mol Med. 2021 Nov;53(11):1759-1768.

Insert

Sox2 (Sox2 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CAG

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pME-3xNLS-(G2SG2)2-Cre (JDW 1149)

Plasmid

#224514

Purpose

A Gateway compatible middle entry clone containing a 3x nuclear localization signal followed by a multiple cloning site and a flexible linker fused to Cre

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

3xNLS-(G2SG2)2-Cre

Use

Cre/Lox

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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p3E-V5-mScarlet-I_SV40pA (JDW 968)

Plasmid

#224529

Purpose

A Gateway compatible 3' entry clone containing an V5 mScarlet fusion followed by SV40 late polyA

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

V5-mScarlet-I

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pRS313_ATP3T911A

Plasmid

#120254

Purpose

Expression of ATP3-6 in yeast

Depositor

Markus Ralser

Article

Vowinckel et al Nat Metab. 2021 Nov;3(11):1521-1535. doi

Insert

ATP3 (ATP3 Budding Yeast)

Expression

Yeast

Mutation

T911 changed to A; Isoleucine 304 changed to Aspa…

Promoter

ATP3

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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mOrange-P2A-hKvb1

Plasmid

#154084

Purpose

Independent expression of mOrange and human Kvbeta 1 under human synapsin 1 promoter

Depositor

Michael Hoppa

Article

Cho et al Proc Natl Acad Sci U S A. 2020 Nov 24;11

Insert

mOrange-P2A-human Kvb1 (KCNAB1 Human, Synthetic)

Use

AAV

Expression

Mammalian

Promoter

human synapsin 1

Available Since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-FLAG-IRES-blasticidin-Sox2 S248A

Plasmid

#182047

Purpose

expression of Sox2 mutants

Depositor

Hyonchol Jang

Article

Kim et al Exp Mol Med. 2021 Nov;53(11):1759-1768.

Insert

Sox2 (Sox2 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

S248A

Promoter

CAG

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-FLAG-IRES-blasticidin-Sox2 T258A

Plasmid

#182048

Purpose

expression of Sox2 mutants

Depositor

Hyonchol Jang

Article

Kim et al Exp Mol Med. 2021 Nov;53(11):1759-1768.

Insert

Sox2 (Sox2 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

T258A

Promoter

CAG

Available Since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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pMEF2c-AHF-Dre-nls (JDW 35)

Plasmid

#242585

Purpose

The Mef2c anterior heart field promoter driving expression of Dre recombinase.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

DRE-nls

Expression

Mammalian

Promoter

mouse Mef2c

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pVB240306

Plasmid

#250660

Purpose

E. coli expression of the GSM domain of mouse ChREBPalpha (aa 43-207)

Depositor

Charles Brenner

Article

Tiwari et al Nat Metab. 2025 Nov;7(11):2284-2299. doi

Insert

ChREBP alpha GSM domain (Mlxipl Mouse)

Tags

6xHis

Expression

Bacterial

Mutation

contains aa 43-307 only, with optimized E. coli c…

Promoter

T7

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pCAGEN-H2B-V5-mScarlet3-S2 (JDW 1529)

Plasmid

#242605

Purpose

A CAGGS driven expression vector containing V5 tagged mScarlet3-S2. H2B for nuclear labeling

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

H2B-mScarlet3-S2

Expression

Mammalian

Promoter

CAGGS

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pCAGEN-H2B-V5-mScarlet3-H (JDW 1530)

Plasmid

#242604

Purpose

A CAGGS driven expression vector containing V5 tagged mScarlet3-H. H2B for nuclear labeling

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

H2B-mScarlet3-H

Expression

Mammalian

Promoter

CAGGS

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pTol2-Fli1-zFUCCI-IRES-mTagBFP2 (JDW 1517)

Plasmid

#242602

Purpose

A Tol2 expression vector containing the fli promoter driving FUCCI to label cell cycle in all blood vessels followed by an IRES-mTagBFP2.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mCerulean-zGem-P2A-mCherry-zCdt1, nls-mTagBFP2

Use

Zebrafish expression

Promoter

fli promoter

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-DV-IRES-myr-EGFP (JDW 495)

Plasmid

#242574

Purpose

Gateway destination vector with a CAGGS promoter in the backbone as well as an IRES-myr-EGFP to label cell membranes.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

attR1-CmR-ccdB-attR2

Expression

Mammalian

Promoter

CAGGS

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pEF1a-EGFP-miR-MCS (JDW 1478)

Plasmid

#242580

Purpose

An EF1a driven expression vector containing an EGFP fused to a microRNA multiple cloning site.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EGFP intron and miR-MCS (empty)

Expression

Mammalian

Promoter

human EF1a

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-FastFUCCI-IRES-myr-mKate2 (JDW 1503)

Plasmid

#242598

Purpose

A CAGGS driven expression vector containing FastFUCCI to label cell cycle state followed by an IRES and a membrane targeted mKate2

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mKO2-hCDT1(30-120) T2A mAG-hGEM(1-110)

Expression

Mammalian

Promoter

CAGGS

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-FastFUCCI-IRES-myr-mTagBFP (JDW 1502)

Plasmid

#242597

Purpose

A CAGGS driven expression vector containing FastFUCCI to label cell cycle state followed by an IRES and a membrane targeted mTagBFP2

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mKO2-hCDT1(30-120) T2A mAG-hGEM(1-110)

Expression

Mammalian

Promoter

CAGGS

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-BLAST-CMV-LiCre-WPRE (JDW 1495)

Plasmid

#242594

Purpose

Lentiviral vector with a CMV promoter driving a light inducible cre (LiCre).

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LiCre

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1 GST-SHP2 H116A E252A

Plasmid

#244493

Purpose

Bacterial Expression of pH insensitive SHP2 mutant

Depositor

Katharine White

Article

Van Dyck et al Sci Signal. 2025 Nov 11;18(912):eadt3018

Insert

PTPN11 (PTPN11 Human)

Tags

GST

Expression

Bacterial

Mutation

changed Histidine 116 to Alanine and Glutamate 25…

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pME-EGFP-hs-miR-126 (JDW 1401)

Plasmid

#242564

Purpose

Gateway middle entry clone containing an EGFP fused to the human microRNA 126.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EGFP with artificial intron encoding miR-126 (MIR126 Human)

Use

Gateway subcloning

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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