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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,838 results for: codon optimized

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Showing: 2401 - 2420 of 4838 results

  1. pCMV-T7-ABE8.20m-SpCas9-NRRH-P2A-EGFP (RMD47)

    Plasmid
    #197499
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRRH(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRRH-BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8.20 mutations in TadA and nSpCas9-NRRH(D10A/I…
    Promoter
    CMV and T7
    Available Since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197499-plasmid-map-sequence-id-386708

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  2. pCMV-T7-ABE8.20m-SpCas9-NRTH-P2A-EGFP (RMD63)

    Plasmid
    #197500
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRTH(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRTH-BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8.20 mutations in TadA and nSpCas9-NRTH(D10A/I…
    Promoter
    CMV and T7
    Available Since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197500-plasmid-map-sequence-id-386637

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  10. pEf1a-goldDn29-dCas9-P2A-GFP

    Plasmid
    #247157
    Purpose
    Mammalian expression of a human codon optimized engineered goldDn29 recombinase fused to dCas9 and gRNA expression cassette for targeting attH1 with H1-g3
    Depositor
    Patrick Hsu
    Article
    Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038
    Insert
    goldDn29-dCas9-P2A-GFP
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N
    Promoter
    Ef1a
    Available Since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    247157-plasmid-map-sequence-id-491683

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  17. VQR variant

    Plasmid
    #138560
    Purpose
    Expresses human codon-optimized SpCas9-VQR and blasticidin resistance: EFS promoter-VQR-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    VQR
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    D1135V, R1335Q, T1337R
    Promoter
    EFS
    Available Since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138560-plasmid-map-sequence-id-274782

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Showing: 2401 - 2420 of 4838 results