We narrowed to 3,590 results for: gfp lenti
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Plasmid#176144PurposeEGFP fused to the N-terminus of POLB, linked by T2A to N-terminus Myc-tagged SIRT6 with the mutation Gly60Ala, a mutation in the PAM site used by SIRT6-KO gRNA1 & a hygromycin resistance cassetteDepositorInsertPolB-T2A-SIRT6 (POLB Human)
UseLentiviralTagsEGFPExpressionMammalianMutationMutation in Gly60 to Ala, and a mutation in the P…PromoterEF1AAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1a-EGFP-PolB-T2A-Myc-SIRT6(PAMmut-R65A)
Plasmid#176143PurposeEGFP fused the N-terminus of POLB, linked by T2A to N-terminus Myc-tagged SIRT6 with the mutation Arg65Ala, a mutation in the PAM site used by SIRT6-KO gRNA1 & a hygromycin resistance cassetteDepositorInsertPolB-T2A-SIRT6 (POLB Human)
UseLentiviralTagsEGFPExpressionMammalianMutationMutation in Arg65 to Ala, and a mutation in the P…PromoterEF1AAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
Multiplex-CRISPR-miR-200FAM-T2A-GFP
Plasmid#179856Purposeto delete miR200 family genes including miR-141, miR-200c, miR-429 and miR-200bDepositorInsertUseCRISPR and LentiviralExpressionMammalianAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE pGK LC3Flux (HaloTag7-mGFP-LC3-STOP)
Plasmid#215015PurposeExpress an autophagy (LC3) reporter in mammalian cells (untested)DepositorAvailable SinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV UBC msNLRP3(WT)-APEX2-3xFLAG-IRES-eGFP
Plasmid#218633PurposeProximity biotinylation of msNLRP3DepositorAvailable SinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-scFv-GCN4-sfGFP-GB1-NLS-dWPRE
Plasmid#60906PurposeThe plasmid encodes an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP and a NLS for imagingDepositorInsertscFv-GCN4
UseLentiviralExpressionMammalianAvailable SinceNov. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTY-EF1a-Hoxa9-p2a-MeisI-p2a-GFP
Plasmid#61738PurposeExpress Hoxa9 and MeisI to induce leukemic transformation. GFP label transduced cells.DepositorInsertHoxa9-MeisI-GFP
UseLentiviralPromoterEF1aAvailable SinceMarch 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(D256A/PAMmut)
Plasmid#176141PurposeEGFP fused to the C-terminus of XRCC1, linked by T2A to N-terminus Myc-tagged POLB with the mutation Asp256Ala, a mutation in the PAM site used by POLBKO gRNA1 & a hygromycin resistance cassetteDepositorUseLentiviralTagsEGFP and MYCExpressionMammalianMutationMyc-tagged PolB with mutation in Asp256 to Ala, a…PromoterEF1AAvailable SinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMOS014: Grx1-roGFP2 glutathione redox sensor (cytosolic)
Plasmid#163055PurposeGrx1-roGFP2 glutathione redox sensor (cytosolic)DepositorInsertGrx1-roGFP2
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS
Plasmid#60904PurposeThe plasmid encodes a antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a transcriptional activation domain VP64DepositorInsertscFv-GCN4
UseLentiviralExpressionMammalianAvailable SinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMOS016: roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)
Plasmid#163058PurposeroGFP2-Orp1 H2O2 oxidation sensor (cytosolic)DepositorInsertroGFP2-Orp1
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pALPS_3'LTR GFP@gag start SFFV rev
Plasmid#101339PurposeLTR drives GFP and internal SFFV promoter drives codon optimized revDepositorInsertGFP, rev
UseLentiviralAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pALPS_3'LTR GFP@gag start SFFV tat
Plasmid#101338PurposeLTR drives GFP and internal SFFV promoter drives codon optimized tatDepositorInsertGFP, tat
UseLentiviralAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV UBC OSBP(PH)-APEX2-3xFLAG-IRES-eGFP
Plasmid#218637PurposeProximity biotinylation control of OSBP(PH domain)DepositorInsertOSBP (OSBP Human)
UseLentiviralTagsAPEX2-3xFLAGExpressionMammalianMutationPH domain onlyPromoterUBCAvailable SinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
BC1209-pHR-dSV40-dCas9-14xGFP11-P2A-BFP
Plasmid#164038PurposeExpression of dCas9-14xGFP11 in mammalian cellsDepositorInsertdCas9-14xGFP11
UseCRISPR and LentiviralExpressionMammalianMutationD10A and H840A in Cas9PromoterdSV40Available SinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
PCDH-CMV-MCS-EF1a-gfp-T2A-puro_HSALNT0217290
Plasmid#197989PurposeFor the overexpression experiments, full length of the transcript (HSALNT0217290) for HSALNG0104472 was inserted.DepositorInsertHSALNT0217290
UseLentiviralTagslacExpressionMammalianAvailable SinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFSW Syt1-C2B V304A,I367A IRES GFP
Plasmid#133821PurposeEncodes the C2B domain of synaptotagmin 1 with membrane-penetrating residue mutations V304A,I367A for viral expressionDepositorInsertSyt1 (Syt1 Rat)
UseLentiviralTagsIRES-GFPMutationV304A, I367APromoterhSyn (human synapsin I promoter)AvailabilityAcademic Institutions and Nonprofits only -
pFSW Syt1-C2A K189-192A IRES GFP
Plasmid#133823PurposeEncodes the C2A domain of synaptotagmin 1 with poly-lysine patch mutations K189-192A for viral expressionDepositorInsertSyt1 (Syt1 Rat)
UseLentiviralTagsIRES-GFPMutationK189-192APromoterhSyn (human synapsin I promoter)AvailabilityAcademic Institutions and Nonprofits only -
pFSW Syt1-C2A M173,F234A IRES GFP
Plasmid#133825PurposeEncodes the C2A domain of synaptotagmin 1 with membrane-penetrating residue mutations M173,F234A for viral expressionDepositorInsertSyt1 (Syt1 Rat)
UseLentiviralTagsIRES-GFPMutationM173, F234APromoterhSyn (human synapsin I promoter)AvailabilityAcademic Institutions and Nonprofits only -
pBS NL4-3 envFS eGFP 5'CMV Δtat ΔTARx2_d2TetOp
Plasmid#101349Purpose2xTet Operator inserted between NFkB and Sp1 sites in U3 of HIV-1 delta tat with 5' CMV-R-U5. 5' and 3' TAR elements were mutated to: 5'-GGTCTCTCTGGTTAGACCAGAAAGGAGCATTGGAGCTCTCTGGCTAACTAGGGAACCC-3DepositorInsertNL4-3
UseLentiviralMutationdelta tat delta TAR delta env GFP in Nef Tet ope…Available SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only