We narrowed to 4,154 results for: pCMV

1. pCMV-T7-BPNLS-ABE8e-iSpyMac-BPNLS-P2A-EGFP (LLH528)

   Plasmid

   #208289

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with iSpyMac(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-ABE8e-iSpyMac-BPNLS-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8e mutations in TadA; mutations in iSpyMac

   Promoter

   CMV and T7

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-protein1-IRES-protein2-IRESatt-EGFP-2A-nTermPuroR-nTermIntein

   Plasmid

   #208378

   Purpose

   Episomal plasmid - for expression of 2 proteins linked with EGFP and n-terminal split puromycin resistance and split intein

   Depositor

   Daniel Hawiger

   Article

   Fallahee et al Antibodies (Basel). 2024 Mar 11;13(1):18

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human CMV

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-T7-BPNLS-CE-ABE8e-SpRY--BPNLS-P2A-EGFP (NK289)

   Plasmid

   #208293

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized CE-ABE8e-SpRY A-to-G base editor and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-CE-ABE8e-SpRY-BPNLS-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8e mutations in TadA inlaid into nSpRY(D10A/A6…

   Promoter

   CMV and T7

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Polymerase-unfused click editor - pCMV-T7-PCV2-nCas9 (LM2702)

   Plasmid

   #208961

   Purpose

   Polymerase-unfused click editor comprised of PCV2 fused to nCas9, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-XTEN-nSpCas9-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A)

   Promoter

   CMV and T7

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Nickase SpCas9 - pCMV-T7-nSpCas9(H840A)-P2A-GFP (HES140)

   Plasmid

   #208976

   Purpose

   Nickase SpCas9 (H840A) with a P2A GFP and 3xFLAG, expressed from a CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   nSpCas9(H840)-BPNLS-P2A-GFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A)

   Promoter

   CMV and T7

   Available since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-TM-KA2-CaM-NES-TevN-AsLOV2-TEVseq-tTA

   Plasmid

   #171615

   Purpose

   Soma-targeted Cal-Light with KA2

   Depositor

   Hyungbae Kwon

   Article

   Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

   Insert

   TM-KA2-CaM-NES-TevN-AsLOV2-TEVseq-tTA
   

   Use

   Synthetic Biology

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPBJ pFOS-KTR-Gal4-PEST-tub3’ pCMV-TagBFP

   Plasmid

   #176531

   Purpose

   PiggyBAC vector expressing a FOS promoter driven "KGV" synthetic transcription factor and a CMV promoter driven BFP marker.

   Depositor

   Jared Toettcher

   Article

   Ravindran et al Cell Syst. 2022 Feb 16;13(2):131-142.e13

   Inserts

   KGV transcription factor
   TagBFP
   

   Use

   Piggybac transposase integration vector

   Tags

   ErkKTR, Gal4 DNA binding domain, PEST destabiliza…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-T7-SpCas9-BPNLS(SV40)-3xFLAG-P2A-mScarlet (MNW001)

   Plasmid

   #174091

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a C-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-mScarlet
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV and T7

   Available since

   Sept. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-T7-ABEmax(7.10)-xCas9(3.7)-P2A-EGFP (RTW4644)

   Plasmid

   #140004

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized ABEmax(7.10) xCas9(3.7) with P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A; xCas9(3.7)=A262T/R324L/S409I/E480K/…

   Promoter

   CMV and T7

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. Control click editor - pCMV-T7-deadPCV2-nCas9-EcKlenow (EMK492)

    Plasmid

    #208943

    Purpose

    A control CE1 construct with catalytically attenuated PCV2 HUH, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    dPCV2-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    PCV2(Y96F); nSpCas9(H840A); EcKlenow(-exo;D355A/E…

    Promoter

    CMV and T7

    Available since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Control click editor - pCMV-T7-PCV2-deadCas9-EcKlenow (EMK500)

    Plasmid

    #208944

    Purpose

    A control CE1 construct with catalytically inactivated SpCas9, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-dSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    dSpCas9(D10A/H840A);EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Polymerase-recruited click editor - pCMV-T7-PCV2-nCas9-CC(N6) (LM2705)

    Plasmid

    #208962

    Purpose

    Polymerase-recruited click editor comprised of PCV2 fused to nCas9 with a C-terminal N6 coiled coil (CC) domain, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-CC(N6)
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A)

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Control click editor - pCMV-T7-PCV2-nCas9-deadEcKlenow (JO520)

    Plasmid

    #208945

    Purpose

    A control CE1 construct with catalytically attenuated EcKlenow DNA polymerase, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-dEcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A);deadEcKlenow(-exo;D355A,D357A,D705…

    Promoter

    CMV and T7

    Available since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-NES-TvmvC-43LK-iLID-TvmvN-TVMVseq(P1'S)-mCherry

    Plasmid

    #210504

    Purpose

    expresses NES-TvmvC-43LK-iLID-TvmvN-TVMVseq(P1'S)-mCherry component in mammalian cells

    Depositor

    Dongmin Lee

    Article

    Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

    Insert

    NES-TvmvC-43LK-iLID-TvmvN-TVMVseq(P1'S)-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-TM-Kv2.1-CaM-NES-TevN-AsLOV2-TEVseq-tTA

    Plasmid

    #171616

    Purpose

    Soma-targeted Cal-Light with Kv2.1

    Depositor

    Hyungbae Kwon

    Article

    Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

    Insert

    TM-Kv2.1-CaM-NES-TevN-AsLOV2-TEVseq-tTA
    

    Use

    Synthetic Biology

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV5 Flag-Par6 delta 250-295 Flag tagged

    Plasmid

    #24646

    Depositor

    Jeff Wrana

    Article

    Narimatsu et al Cell. 2009 Apr 17. 137(2):295-307.

    Insert

    Par6c (Pard6a Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Deletion of the PDZ domain of Par6c (aa 250-295)

    Promoter

    Available since

    June 2, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pCMV CHMP2B-L4D,F5D-V82E M85E M92E-3XHA

    Plasmid

    #242418

    Purpose

    Expression of a CHMP2B membrane binding mutant (L4D F5D) with V82E, M85E, and M92E mutations and a 3xHA tag.

    Depositor

    Franz-Ulrich Hartl

    Insert

    CHMP2B (CHMP2B Human)
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    L4D F5D V82E M85E M92E

    Promoter

    CMV

    Available since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-scFv-GCN4-StayGold-IRESv4-HaloTag-tdMCP-CAAX

    Plasmid

    #241144

    Purpose

    Expresses Anti-GCN4 scFv-StayGold, HaloTag-tdMCP, and HaloTag-tdMCP-CAAX to track mature and nascent HA-tagged proteins and track MS2-tagged mRNA anchored to the plasma membrane

    Depositor

    Tim Stasevich

    Article

    Galindo et al Mol Biol Cell. 2025 Jun 1;36(6):mr6. doi

    Inserts

    Anti-GCN4 scFv
    tdMCP - CAAX
    

    Use

    Tags

    (n2)oxStayGold(c4) E147D and HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV CHMP2B-L4D F5D-d106-113-3xHA (Δα3)

    Plasmid

    #232007

    Purpose

    Expression of a CHMP2B membrane binding mutant (L4D F5D) with the third alpha helix deleted with a 3xHA tag.

    Depositor

    Franz-Ulrich Hartl

    Insert

    CHMP2B (CHMP2B Human)
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    L4D F5D Δ106-113

    Promoter

    Available since

    Aug. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV CHMP2B-L4D F5D-d118-138-3xHA (Δα4)

    Plasmid

    #232008

    Purpose

    Expression of a CHMP2B membrane binding mutant (L4D F5D) with the fourth alpha helix deleted with a 3xHA tag.

    Depositor

    Franz-Ulrich Hartl

    Insert

    CHMP2B (CHMP2B Human)
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    L4D F5D Δ118-138

    Promoter

    Available since

    Aug. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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