We narrowed to 2,501 results for: ERK

1. pET29b-AK1-His

   Plasmid

   #124135

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Adenylate kinase (AK1 Chicken)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUAS-V5-TurboID-NES

   Plasmid

   #116904

   Purpose

   Expresses TurboID under Gal4/UAS control. TurboID is tagged at the N-terminus with V5 and a nuclear export signal (NES) at the C-terminus.

   Depositor

   Norbert Perrimon

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   TurboID
   

   Use

   Drosophila expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNW-sfGFP(SPS6)

   Plasmid

   #133814

   Purpose

   Fluorescent protein sfGFP(SPS6) variant for optimized expression in Bacillus mycoides.

   Depositor

   Oscar Kuipers

   Article

   Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

   Insert

   sfGFP(SPS6)
   

   Use

   E.coli/bacillus spp. shuttle vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ppta

   Available since

   Nov. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJCC_076 AsCas12a R1226A

   Plasmid

   #171669

   Purpose

   For bacterial expression of AsCas12a R1226A (RuvC-inhibiting mutation) with an N-terminal His-MBP tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

   Insert

   AsCas12a R1226A
   

   Use

   Tags

   10X His, TEV protease cleavage site, and maltose-…

   Expression

   Bacterial

   Mutation

   changed arginine 1226 to alanine

   Promoter

   Available since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJCC_075 AsCas12a D908A

   Plasmid

   #171668

   Purpose

   For bacterial expression of AsCas12a D908A (RuvC-inactivating mutation) with an N-terminal His-MBP tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

   Insert

   AsCas12a D908A
   

   Use

   Tags

   10X His, TEV protease cleavage site, and maltose-…

   Expression

   Bacterial

   Mutation

   changed aspartate 908 to alanine

   Promoter

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGex-3x MKK4

   Plasmid

   #14617

   Depositor

   Roger Davis

   Article

   Derijard et al Science. 1995 Feb 3. 267(5198):682-5.

   Insert

   MKK4 (MAP2K4 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 25, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pJCC_058 FnCas12a D917A

   Plasmid

   #171667

   Purpose

   For bacterial expression of FnCas12a D917A (RuvC-inactivating mutation) with a C-terminal sorting motif and His tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

   Insert

   FnCas12a D917A
   

   Use

   Tags

   6X His and sortase A sorting motif

   Expression

   Bacterial

   Mutation

   changed aspartate 917 to alanine

   Promoter

   Available since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. TAL2324

   Plasmid

   #36750

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   MAP2K4-TALEN_Left (Map2k4 Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Expression

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

9. TAL2325

   Plasmid

   #36751

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   MAP2K4-TALEN_Right (Map2k4 Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Expression

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pJCC_103 SpCas9 T1337C

    Plasmid

    #179525

    Purpose

    For bacterial expression of SpCas9 T1337C (for DNA cross-linking) with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

    Insert

    SpCas9 T1337C
    

    Use

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Mutation

    changed threonine 1337 to cysteine

    Promoter

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Sc NybbR eRF3∆165N (H348E)

    Plasmid

    #175420

    Purpose

    Encodes His-MBP-TEV-ybbR-H348E eRF3∆165N

    Depositor

    Joseph Puglisi

    Article

    Lawson et al Science. 2021 Aug 20;373(6557):876-882.

    Insert

    eRF3 (SUP35 Budding Yeast)
    

    Use

    Tags

    His-MBP-TEV and ybbR

    Expression

    Bacterial

    Mutation

    ∆165N, H348E

    Promoter

    T7

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. KHBD00487

    Plasmid

    #39592

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG1048 (zen2 Fly)
    

    Use

    Gateway donr vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Sept. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pCDNA3 Flag MKK4

    Plasmid

    #14615

    Depositor

    Roger Davis

    Article

    Derijard et al Science. 1995 Feb 3. 267(5198):682-5.

    Insert

    MKK4 (MAP2K4 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCDNA3 Flag MKK4(glu)

    Plasmid

    #14813

    Depositor

    Roger Davis

    Article

    Whitmarsh et al Science. 1995 Jul 21. 269(5222):403-7.

    Insert

    MKK4(glu) (MAP2K4 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser-220Glu Thr-224Glu

    Promoter

    Available since

    July 25, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pCDNA3 Flag MKK4(ala)

    Plasmid

    #15314

    Depositor

    Roger Davis

    Article

    Whitmarsh et al Science. 1995 Jul 21. 269(5222):403-7.

    Insert

    MKK4(ala) (MAP2K4 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser-220Ala Thr-224Ala

    Promoter

    Available since

    July 25, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pTA231-p.Syn-PAmCherry1Hfx

    Plasmid

    #164661

    Purpose

    expression of H. volcanii codon-optimized PAmCherry1

    Depositor

    Anita Marchfelder

    Article

    Turkowyd et al Front Microbiol. 2020 Nov 20;11:583010.

    Insert

    PAmCherry1Hfx
    

    Use

    Archaeal expression

    Tags

    Expression

    Mutation

    codon-optimization for Haloferax volcanii

    Promoter

    p.Syn

    Available since

    Feb. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA3 Flag MKK4beta

    Plasmid

    #15517

    Depositor

    Roger Davis

    Article

    Derijard et al Science. 1995 Feb 3. 267(5198):682-5.

    Insert

    pCDNA3 Flag MKK4beta (MAP2K4 Human)
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 24, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pTA231-p.Syn-Dendra2Hfx

    Plasmid

    #164660

    Purpose

    expression of H. volcanii codon-optimized Dendra2

    Depositor

    Anita Marchfelder

    Article

    Turkowyd et al Front Microbiol. 2020 Nov 20;11:583010.

    Insert

    Dendra2Hfx
    

    Use

    Archaeal expression

    Tags

    Expression

    Mutation

    codon-optimization for Haloferax volcanii

    Promoter

    p.Syn

    Available since

    Feb. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAC-luc-CLK

    Plasmid

    #33132

    Depositor

    Michael Rosbash

    Article

    Kadener et al Genes Dev. 2009 Sep 15;23(18):2179-91. E

    Insert

    CLK 3' UTR (Clk Fly)
    

    Use

    Luciferase

    Tags

    Firefly Luciferase

    Expression

    Insect

    Mutation

    Promoter

    Actin 5C

    Available since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

20. HA SEK AL pcDNA3.1 zeo (+) - SEK1 (A-L)

    Plasmid

    #24653

    Depositor

    Jim Woodgett

    Article

    Yan et al Nature. . 372(6508):798-800.

    Insert

    SEK1 (MAP2K4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Ser220Ala, Thr224Leu

    Promoter

    Available since

    June 21, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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