We narrowed to 12,818 results for: mel

1. pUb FLAG-Mts H241A

   Plasmid

   #208404

   Purpose

   Expresses FLAG-tagged Drosophila mts protein with H241A mutation

   Depositor

   Jeremy Wilusz

   Article

   Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

   Insert

   microtubule star (mts Fly)
   

   Use

   Tags

   3xFLAG

   Expression

   Insect

   Mutation

   H241A

   Promoter

   Ubi-p63e

   Available since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pnCS_DSep2_Peanut-TEV-Strep

   Plasmid

   #174495

   Purpose

   bacterial co-expression of Drosophila Sep2 and of Drosophila Peanut

   Depositor

   Manos Mavrakis

   Article

   Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

   Inserts

   Sep2 (Sep2 Fly)
   peanut (pnut Fly)
   

   Use

   Tags

   TEV-Strep

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCSC-CMV-HA-Ast-3-NP-1-IRES-mCherry

   Plasmid

   #159631

   Purpose

   expresses HA Allatostatin-3, Neurophysin-1, IRES mCherry under the CSC promoter

   Depositor

   Ross Bathgate, Andrew Allen

   Article

   Ngo et al Cell Rep. 2020 Sep 15;32(11):108139. doi

   Insert

   Ast (AstA Fly)
   

   Use

   Lentiviral

   Tags

   HA, IRES, mCherry, and neurophysin-1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLX313-TP53-WT

   Plasmid

   #118014

   Purpose

   Constitutive expression of wild-type human Tumor Protein p53 (TP53)

   Depositor

   David Root, William Hahn

   Article

   Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

   Insert

   TP53 (TP53 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. MEK1 (S218D,S222D)-pcw107

   Plasmid

   #64604

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   Kris Wood, David Sabatini

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   MAP2K1 (MAP2K1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   S218D, S222D

   Promoter

   PGK

   Available since

   June 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLX313-TP53-P278A

   Plasmid

   #118015

   Purpose

   Constitutive expression of mutant human Tumor Protein p53 (TP53)

   Depositor

   David Root, William Hahn

   Article

   Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

   Insert

   TP53 (TP53 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   p.Pro278Ala

   Promoter

   EF1alpha

   Available since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pXPR003-sgTP53-2

   Plasmid

   #118020

   Purpose

   Constitutive expression of sgRNA targeting human TP53

   Depositor

   David Root, William Hahn

   Article

   Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

   Insert

   sgTP53-2 (TP53 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pXPR003-sgTP53-4

   Plasmid

   #118022

   Purpose

   Constitutive expression of sgRNA targeting human TP53

   Depositor

   David Root, William Hahn

   Article

   Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

   Insert

   sgTP53-4 (TP53 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pXPR003-sgTP53-1

   Plasmid

   #118019

   Purpose

   Constitutive expression of sgRNA targeting human TP53

   Depositor

   David Root, William Hahn

   Article

   Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

   Insert

   sgTP53-1 (TP53 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pXPR003-sgTP53-3

    Plasmid

    #118021

    Purpose

    Constitutive expression of sgRNA targeting human TP53

    Depositor

    David Root, William Hahn

    Article

    Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

    Insert

    sgTP53-3 (TP53 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. MEK1-pcw107-V5

    Plasmid

    #64650

    Purpose

    (control) when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    MAP2K1 (MAP2K1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    PGK

    Available since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pXPR003-sgTP53-5

    Plasmid

    #118023

    Purpose

    Constitutive expression of sgRNA targeting human TP53

    Depositor

    David Root, William Hahn

    Article

    Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

    Insert

    sgTP53-5 (TP53 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. TP53 MITE Library

    Pooled Library

    #113569

    Purpose

    Pooled lentiviral plasmid library containing ~8,000 single amino acid substitution and nonsense mutant variants of p53

    Depositor

    David Root, William Hahn

    Article

    Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    Lentiviral

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA-Reb

    Plasmid

    #66792

    Purpose

    Expression of D. melanogaster CG13183

    Depositor

    Marta Llimargas Casanova

    Insert

    CG13183 (CG13183 Fly)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pHD-dsRed-attP_dCrk_flankingHRs

    Plasmid

    #131141

    Purpose

    Contains ~1kb homology regions flanking Drosophila crk locus. Used to make crk null mutant. Homology regions cloned into AarI and SapI sites of pHD-dsRed-attP (Harrison, O'Connor-Giles, Wildonger).

    Depositor

    Mark Peifer

    Article

    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

    Inserts

    Crk Upstream Homology Region (Crk Fly)
    Crk Downstream Homology Region (Crk Fly)
    

    Use

    CRISPR and Cre/Lox; Homology directed repair temp…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAc5.1B-EGFP-DmUNR_AA

    Plasmid

    #148205

    Purpose

    Insect Expression of DmUNR

    Depositor

    Elisa Izaurralde

    Insert

    DmUNR (Unr Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAc5.1B-lambdaN-HA-DmUNR_AA

    Plasmid

    #148232

    Purpose

    Insect Expression of DmUNR

    Depositor

    Elisa Izaurralde

    Insert

    DmUNR (Unr Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEM-Teasy-Exp

    Plasmid

    #66791

    Purpose

    Expression of D. melanogaster CG13188

    Depositor

    Marta Llimargas Casanova

    Insert

    CG13188 (CG13188 Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    H452N (see depositor's comments below)

    Promoter

    CMV

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

19. Fly 2.0 pIDTBlue

    Plasmid

    #117418

    Purpose

    Drosophila melanogaster gene cloned into pIDTBlue vector for internal extraction control

    Depositor

    Dirk Dittmer

    Insert

    FLY 2.0
    

    Use

    Tags

    NA

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-His6-ERK2-MEK1_R4F_coexpression

    Plasmid

    #39212

    Depositor

    Melanie Cobb

    Article

    Khokhlatchev et al J Biol Chem. 1997 Apr 25;272(17):11057-6

    Inserts

    Mek1 R4F (MAP2K1 Human)
    Erk2 (Mapk1 Rat)
    

    Use

    Tags

    His6 and RBS

    Expression

    Bacterial

    Mutation

    MEK1(R4F) = S218E, S222D and deletion of AA32-51

    Promoter

    T7 and T7 via RBS

    Available since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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