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pME_G_0_1-2_007_OC5'7

Plasmid

#235807

Purpose

5' Operon Connector 7 in the M1 position used to construct operons

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Operon Connector 7

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pME_G_0_2_001_Promoter_placeholder

Plasmid

#235808

Purpose

Encodes a sfGFP cassette in the promoter position used for placeholder cloning

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

Promoter placeholder

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pME_B_0_2_020_Pdummy

Plasmid

#235809

Purpose

Promoter dummy sequence used as a baseline for promoter characterization

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

Promoter dummy

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pME_G_0_1-2_002_OC5'2

Plasmid

#235802

Purpose

5' Operon Connector 2 in the M1 position used to construct operons

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Operon Connector 2

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pME_G_0_1-2_003_OC5'3

Plasmid

#235803

Purpose

5' Operon Connector 3 in the M1 position used to construct operons

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Operon Connector 3

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pME_G_0_1-2_005_OC5'5

Plasmid

#235805

Purpose

5' Operon Connector 5 in the M1 position used to construct operons

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Operon Connector 5

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pME_G_0_1-2_006_OC5'6

Plasmid

#235806

Purpose

5' Operon Connector 6 in the M1 position used to construct operons

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Operon Connector 6

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pME_G_0_1-2_001_OC5'1

Plasmid

#235801

Purpose

5' Operon Connector 1 in the M1 position used to construct operons

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Operon Connector 1

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_7-8_004_Amp/ColEI_sfGFP

Plasmid

#235970

Purpose

Ampicillin/ColEI backbone with sfGFP placeholder for lvl1 assembly

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

Amp/ColEI

Use

Synthetic Biology

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pET28b-TREER

Plasmid

#208321

Purpose

Bacterial expression of the His-tagged peptide TREER.

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TREER

Tags

DBCO-Tag, His-Tag

Expression

Bacterial

Available Since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pET28b-PuRRRE

Plasmid

#208323

Purpose

Bacterial expression of the His-tagged peptide PuRRRE.

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

PuRRRE

Tags

DBCO-Tag, His-Tag

Expression

Bacterial

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pEsg1-ps-6K-Luc

Plasmid

#15395

Depositor

Shinya Yamanaka

Article

Amano et al BMC Dev Biol. 2006 Feb 28;6:11. doi: 10.

Insert

Upstream region of mouse Esg1 pseudogene

Use

Luciferase

Available Since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only
Exp-pcDNA3.2delCMV(EF1α-tTA/TetO-mCh-Rs1)

Plasmid

#26797

Purpose

Human 5HT4B receptor with D100A mutation, generating the RASSL Rs1. Construct expresses both mCherry and Rs1 via a P2A ribosomal skip sequence. Expresses tTA under EF1α promoter separately.

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Inserts

Rs1 (HTR4 Human)
tTA

Tags

mCherry-P2A

Expression

Mammalian

Mutation

D100A

Promoter

EF1α and short TetO, miniCMV

Available Since

Feb. 23, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PPO-Venus

Plasmid

#139502

Purpose

Expresses Venus-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.

Depositor

Michael Bruchas

Article

Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

Insert

parapinopsin

Tags

Venus tag following 3x Ala linker

Expression

Mammalian

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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STARR-seq luciferase validation vector_SCP1_empty

Plasmid

#99299

Purpose

Luciferase validation vector; empty vector without insert to determine baseline

Depositor

Alexander Stark

Article

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Type

Empty backbone

Use

Luciferase; Starr-seq luciferase validation vector

Expression

Mammalian

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pSPORT1[NheI/glGFP/bglobin3'-UTR/Ins2]

Plasmid

#74101

Purpose

vector bearing an NheI restriction site for the two-step cloning method in Wang and Szaro, Dev Biol, 2015

Depositor

Ben Szaro

Article

Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:

Inserts

Green Lantern Green Fluorescent Protein
Rabbit beta 1-globin gene 3'UTR

Available Since

May 26, 2016

Availability

Academic Institutions and Nonprofits only

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pReporter_5

Plasmid

#60566

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_5

Expression

Bacterial

Promoter

BBa_J23119

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pCFJ1656

Plasmid

#51483

Purpose

miniMos transposable element with HygroR and peel-1, R4-R3 Gateway cloning

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Type

Empty backbone

Expression

Worm

Available Since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

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pLentiRNACRISPR_010-TetO-NLS-RfxCas13d-2A-mCherry-NLS-WPRE-EFS-rtTA3-2A-Blast

Plasmid

#228555

Purpose

Expresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus

Depositor

Neville Sanjana

Article

Hart et al Nat Biotechnol. 2025 Feb 11. doi: 10.103

Insert

RfxCas13d

Use

Lentiviral

Tags

NLS-HA and P2A-mCherry

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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3xFlag-tdMCP-Erk2 (pErk reporter)

Plasmid

#226167

Purpose

Used to transiently express the pErk reporter in LABEL-seq MAPK activity assays.

Depositor

Dustin Maly

Article

Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

Insert

3xFlag-tdMCP-Erk2 (MAPK1 Human)

Tags

3xFlag-tdMCP

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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