We narrowed to 10,949 results for: aga

1. pKLV2-U6gRNA5(SOX2_v32_7-5)-PGKpuroBFP-W

   Plasmid

   #211987

   Purpose

   Express gRNA against SOX2 with puro and BFP

   Depositor

   Kosuke Yusa

   Article

   Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

   Insert

   sgRNA targeting SOX2 (SOX2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6 promoter

   Available since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC57-2xFLAG-mAID-AMBRA1 HDR donor template

   Plasmid

   #172609

   Purpose

   Donor plasmid containing the HDR template for the in-frame, N-terminal 2xFLAG-mAID tagging of AMBRA1 gene

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   2xFLAG-mAID-AMBRA1 (AMBRA1 Synthetic, Human)
   

   Use

   CRISPR; Human targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. P932_CLYBL-CBX3-Cbh-Zeo-TetO-NEUROG2

   Plasmid

   #202762

   Purpose

   Encodes one forebrain neuron specific transcription factor under the control of a TetOn promoter at the CLYBL safe harbor

   Depositor

   Karl Wahlin

   Article

   Agarwal et al NPJ Regen Med. 2023 Sep 29;8(1):55. doi:

   Insert

   NEUROG2 (NEUROG2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TetO

   Available since

   Feb. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-FLAG_FBXL7 (R310H)

   Plasmid

   #171849

   Purpose

   pcDNA3-FLAG_FBXL7 (R310H)

   Depositor

   Michele Pagano

   Article

   Moro et al Nat Cell Biol. 2020 Sep;22(9):1130-1142.

   Insert

   F-box and leucine rich repeat protein 7 (FBXL7 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   changed Arginine 310 to Histidine

   Promoter

   Available since

   Nov. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBABE-2xFLAG-2xSTREP-CCND3-Puro

   Plasmid

   #172622

   Purpose

   Retroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D3 and a puromycin resistance marker in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND3 (CCND3 Human)
   

   Use

   Retroviral

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBABE-2xFLAG-2xSTREP-CCND2-Puro

   Plasmid

   #172628

   Purpose

   Retroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D2 and a puromycin resistance marker in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND2 (CCND2 Human)
   

   Use

   Retroviral

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMXs-ms-c-Myc-L420P

   Plasmid

   #50776

   Purpose

   retroviral expression of mouse c-Myc L420P mutant

   Depositor

   Shinya Yamanaka

   Article

   Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

   Insert

   c-Myc L420P (MYC, Myc Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   L420P (does not bind to Max)

   Promoter

   Available since

   Feb. 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-2xFLAG-2xSTREP-CCND1(P287S)-IRES-mCherry

   Plasmid

   #172635

   Purpose

   Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1(P287S) and mCherry in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Lentiviral

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Pro287Ser

   Promoter

   CMV

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-AICD-NLS-IRES-hrGFP

   Plasmid

   #107543

   Purpose

   AAV-mediated expression of AICD (last 50 amino acids of APP) with Nuclear Localisation Signal (NLS: CCAAAAAAGAAGAGAAAGGTA) at C-term end and IRES-mediated co-expression of hrGFP

   Depositor

   Hélène Marie

   Article

   Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

   Insert

   AICD-NLS (APP Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   None

   Promoter

   CMV

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMSCV-FLAG-2xSTREP-CCND2(T280A)-Puro

    Plasmid

    #172625

    Purpose

    Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D2(T280A) and a puromycin resistance marker in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND2 (CCND2 Human)
    

    Use

    Retroviral

    Tags

    FLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Thr280Ala

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMSCV-FLAG-2xSTREP-CCND1-Puro

    Plasmid

    #172643

    Purpose

    Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D1 and a puromycin resistance marker in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Retroviral

    Tags

    FLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOTTC1270 - pAAV Rosa26 gRNA A+B EF1a EGFP

    Plasmid

    #113156

    Purpose

    An AAV vector that expresses guide RNAs targeting rat Rosa26 and expresses EGFP reporter

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    Two gRNAs for rat Rosa26
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 and hU6

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMRX-IP-EGFP-PA-BAX(ER, G108V)

    Plasmid

    #233344

    Purpose

    Stable expression of EGFP-LOV2(N538E)-BAX(G108V)-Cyb5a in mammalian cells. The G108V mutation suppresses pore-forming activity of BAX.

    Depositor

    Noboru Mizushima

    Article

    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

    Inserts

    LOV2 domain of phototropin1
    BAX (BAX Human)
    Cyb5a (Cyb5a Rat)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    BAX(3-171), N-terminus and C-terminus are deleted…

    Promoter

    Available since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. P527_POU4F2-p2A-mNeonGreen-h2b

    Plasmid

    #239103

    Purpose

    Azenta NGS; inserts a p2A-mNeonGreen-h2b fusion at the endogenous POU4F2 (aka BRN3B) gene

    Depositor

    Karl Wahlin

    Article

    Agarwal et al NPJ Regen Med. 2023 Sep 29;8(1):55. doi:

    Insert

    POU4F2 (POU4F2 Human)
    

    Use

    Tags

    p2A-mNeonGreen-h2b

    Expression

    Mammalian

    Mutation

    Promoter

    Endogenous POU4F2

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMRX-IP-EGFP-PA-BAX(mito, G108V)

    Plasmid

    #233343

    Purpose

    Stable expression of EGFP-LOV2(N538E)-BAX(G108V)-OMP25 in mammalian cells. The G108V mutation suppresses pore-forming activity of BAX.

    Depositor

    Noboru Mizushima

    Article

    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

    Inserts

    LOV2 domain of phototropin1
    BAX (BAX Human)
    Omp25 (Synj2bp Rat)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    N-terminus and C-terminus are deleted. BAX(3-171)…

    Promoter

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMRX-IP-EGFP-PA-BAX(lyso, G108V)

    Plasmid

    #233345

    Purpose

    Stable expression of EGFP-LOV2(G528A, N538E)-BAX-TMEM106B in mammalian cells. The G108V mutation suppresses pore-forming activity of BAX.

    Depositor

    Noboru Mizushima

    Article

    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

    Inserts

    LOV2 domain of phototropin1
    BAX (BAX Human)
    TMEM106B (TMEM106B Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    BAX(3-171), N-terminus and C-terminus are deleted…

    Promoter

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMRX-IP-EGFP-PA-BAX(lyso)

    Plasmid

    #228939

    Purpose

    Stable expression of EGFP-LOV2(G528A, N538E)-BAX-TMEM106B in mammalian cells. Photoactivatable BAX localizes on lysosomal membrane and induces lysosomal rupture upon blue light stimulation.

    Depositor

    Noboru Mizushima

    Article

    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

    Inserts

    LOV2 domain of phototropin1
    BAX (BAX Human)
    TMEM106B (TMEM106B Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    N-terminus and C-terminus are deleted. BAX(3-171)…

    Promoter

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-FLEX-CAG_G-PTEN

    Plasmid

    #227438

    Purpose

    An AAV backbone for expression of the G-PTEN sensor under the CAG promoter, in a Cre dependent manner.

    Depositor

    Tal Laviv

    Article

    Kagan et al Nat Methods. 2025 Apr;22(4):764-777. doi

    Insert

    G-PTEN sensor (Pten Rat)
    

    Use

    AAV

    Tags

    CD-sREACh and mEGFP

    Expression

    Mammalian

    Mutation

    R14G

    Promoter

    pCAG

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1 FFSS-BACH1 C122E

    Plasmid

    #232274

    Purpose

    Expression of N-terminal tagged 2xFLAG-2xSTREP-BACH1 (H. sapiens) with Cys122 to Glu point mutation in human cell lines

    Depositor

    Michele Pagano

    Article

    Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

    Insert

    BACH1 (BACH1 Human)
    

    Use

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    changed cysteine 122 to glutamic acid

    Promoter

    CMV

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. sgMST1/2-1

    Plasmid

    #229429

    Purpose

    knockout of MST1 and MST2

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    sgMST1/2-1 (MST1, STK3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6;bU6;EFS

    Available since

    Dec. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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