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pPB-[EF1a-mRuby2-bGH]-UbC-PuroR-T2A-mGL-SV40pA

Plasmid

#235325

Purpose

PiggyBac integration vector for ComMAND base gene regulating mRuby2

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology; Piggybac

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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XZ079 (PB)EF1a-CCR6-ADARdd-P2A-PuroR-IRES-ARRB2-MCP

Plasmid

#233435

Purpose

PiggyBac donor plasmid with CCR6-V2-ADAR2dd(E488Q,T501A) and ARRB2-tdMCP separated by EMCV IRES

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

CCR6-ADARdd-P2A-PuroR-IRES-ARRB2-MCP

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

EF1a

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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LSM042 (PB)EF1a-AVPR-ADARdd-P2A-PuroR-IRES-ARRB2-MCP

Plasmid

#233426

Purpose

PiggyBac donor plasmid AVPR2-V2-ADAR2dd(E488Q,T501A) and ARRB2-tdMCP separated by EMCV IRES

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

AVPR-ADARdd-P2A-PuroR-IRES-ARRB2-MCP

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

EF1a

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-NLS-msfGFP-T2A-mCherry-NES> mPGK-PuroR

Plasmid

#218916

Purpose

Lentiviral construct expressing msfGFP and nuclear mCherry proteins.

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

EF1a-msfGFP-T2A-mCherry-NLS > mPGK-PuroR

Use

Lentiviral

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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pW228-lenti-sg1-mmSerpinh1-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#189944

Purpose

Lentiviral vector to co-express a mouse Serpinh1 spsgRNA (sg1-Serpinh1) with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

Insert

NLS-mScarlet-I-P2A-BlastR; Serpinh1 spsgRNA #1

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pW229-lenti-sg2-mmSerpinh1-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#189945

Purpose

Lentiviral vector to co-express a mouse Serpinh1 spsgRNA (sg2-Serpinh1) with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

Insert

NLS-mScarlet-I-P2A-BlastR; Serpinh1 spsgRNA #2

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pW230-lenti-sg3-mmSerpinh1-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#189946

Purpose

Lentiviral vector to co-express a mouse Serpinh1 spsgRNA (sg3-Serpinh1) with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

Insert

NLS-mScarlet-I-P2A-BlastR; Serpinh1 spsgRNA #3

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pW317-lenti-sg1-mmItga9-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#189947

Purpose

Lentiviral vector to co-express a mouse Itga9 spsgRNA (sg1-Itga9) with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

Insert

NLS-mScarlet-I-P2A-BlastR; Itga9 spsgRNA #1

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pW318-lenti-sg2-mmItga9-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#189948

Purpose

Lentiviral vector to co-express a mouse Itga9 spsgRNA (sg2-Itga9) with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

Insert

NLS-mScarlet-I-P2A-BlastR; Itga9 spsgRNA #2

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pW319-lenti-sg3-mmItga9-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#189949

Purpose

Lentiviral vector to co-express a mouse Itga9 spsgRNA (sg3-Itga9) with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

Insert

NLS-mScarlet-I-P2A-BlastR; Itga9 spsgRNA #3

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pW401-lenti-sg1-mmItgb1-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#189950

Purpose

Lentiviral vector to co-express a mouse Itgb1 spsgRNA (sg1-Itgb1) with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

Insert

NLS-mScarlet-I-P2A-BlastR; Itgb1 spsgRNA #1

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pW402-lenti-sg2-mmItgb1-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#189951

Purpose

Lentiviral vector to co-express a mouse Itgb1 spsgRNA (sg2-Itgb1) with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

Insert

NLS-mScarlet-I-P2A-BlastR; Itgb1 spsgRNA #2

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pW403-lenti-sg3-mmItgb1-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#189952

Purpose

Lentiviral vector to co-express a mouse Itgb1 spsgRNA (sg3-Itgb1) with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

Insert

NLS-mScarlet-I-P2A-BlastR; Itgb1 spsgRNA #3

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(PAMmut)

Plasmid

#176139

Purpose

EGFP fused to the C-terminus of XRCC1, linked by T2A to N-terminus Myc-tagged POLB with a mutation in the PAM site used by POLBKO gRNA1 & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

XRCC1-T2A-PolB (XRCC1, POLB Human)

Use

Lentiviral

Tags

EGFP and MYC

Expression

Mammalian

Promoter

EF1A

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pW188-lenti-spsgRNA-lacZ-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#170813

Purpose

Lentiviral vector to co-express a lacZ control spsgRNA with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

Insert

NLS-mScarlet-I-P2A-BlastR

Use

Lentiviral

Expression

Mammalian

Mutation

NA

Available Since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pJM592 EF1α 3xFLAG-NLS-DsDed-ZF10-intN-VP64 in TUPV2

Plasmid

#161568

Purpose

Constitutive expression of 3xFLAG-NLS-DsDed-ZF10-intN-VP64 under the EF1α promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-DsDed-ZF10-intN-VP64

Use

Synthetic Biology

Tags

3x-FLAG

Expression

Mammalian

Promoter

EF1α

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pJM524 EF1α 3xFLAG-NLS-VP64-intC-ZF1-NLS-HA in TUPV2

Plasmid

#161553

Purpose

Constitutive expression of 3xFLAG-NLS-VP64-intC-ZF1-NLS-HA under the EF1α promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-VP64-intC-ZF1-NLS-HA

Use

Synthetic Biology

Tags

3x-FLAG and HA

Expression

Mammalian

Promoter

EF1α

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pJM525 EF1α 3xFLAG-NLS-DsDed-intC-ZF1-NLS-HA in TUPV2

Plasmid

#161554

Purpose

Constitutive expression of 3xFLAG-NLS-DsDed-intC-ZF1-NLS-HA under the EF1α promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-DsDed-intC-ZF1-NLS-HA

Use

Synthetic Biology

Tags

3x-FLAG and HA

Expression

Mammalian

Promoter

EF1α

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pSLQ4428 pHR (EF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE)

Plasmid

#214885

Purpose

Lentiviral vector encoding TMP-regulatable RfxCas13d-DD fusion

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Insert

EF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EF1a

Available Since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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pSLQ9834_sgBB: pHR-hU6-CasMINI sgRNA_#2; EF1a-Puro-T2A-BFP- WPRE

Plasmid

#180280

Purpose

The CasMINI sgRNA cloning backbone with sites 'BsmBI' for inserting new guide sequences.

Depositor

Stanley Qi

Article

Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

Insert

CasMINI sgRNA (backbone) and BFP

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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