We narrowed to 10,670 results for: nar;
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Plasmid#176226PurposeA helper plasmid for expression of the artificial T7ocr-gam-bet-exo operon. The plasmid carries the chloramphenicol acetyltransferase gene repressible by the CI repressor of lambda phage.DepositorInsertLambda Red-T7ocr operon under control of PrhaB promoter and cat under control of PL promoter of lambda phage
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPrhaB and PLAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVE13300
Plasmid#137715PurposeExpresses impLbCas12a in mammalian cells.DepositorInsertimpLbCas12a
UseTags3xHA, NLS, and SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
LLP775_αGCN4-p65HSF1-CO
Plasmid#211768PurposeSunTag counterpart binding domain, aGCN4, fused to transcriptional activator p65HSF1, with GFP selectionDepositorInsertaGCN4-p65HSF1
UseTags3xTy1ExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect p65HSF…PromoterpEF1a and pSV40Available sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
LLP777_SnoopCatcher-p65HSF1-CO
Plasmid#211770PurposeSnoopTag counterpart binding domain, SnoopCatcher, fused to transcriptional activator p65HSF1, with GFP selectionDepositorInsertSnoopCatcher-p65HSF1
UseTags2xOLLASExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect p65HSF…PromoterpEF1a and pSV40Available sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cas9-sgRNA-A
Plasmid#149369PurposeExpresses SpCas9 and sgRNA targeting the lenti-CDDR reporterDepositorInsertsCas9
sgRNA-A
Puromycin resistance
UseCRISPRTags3xFLAG, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationPromoterCBh, PGK, and U6Available sinceNov. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
LLP778_Traptavidin-p65HSF1-CO
Plasmid#211771PurposeAviTag counterpart binding domain, Traptavidin, fused to transcriptional activator p65HSF1, with sfGFP selectionDepositorInsertTraptavidin-p65HSF1
UseTags3xV5ExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect p65HSF…PromoterpEF1a and pSV40Available sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
LLP776_SpyCatcher-p65HSF1-CO
Plasmid#211769PurposeSpyTag counterpart binding domain, SpyCatcher, fused to transcriptional activator p65HSF1, with GFP selectionDepositorInsertSpyCatcher-p65HSF1
UseTags3xFLAGExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect p65HSF…PromoterpEF1a and pSV40Available sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
lenti F9-TetR-EGFP-IRES-PuroR
Plasmid#117049Purposelentiviral vector for expression of TetR-EGFP, for visualization of TetO repeatsDepositorInsertF9-TetR-EGFP
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterF9 promoterAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2 NT sgRNA3
Plasmid#164929PurposeExpresses Non-targeting sgRNA3 control in mammalian cellsDepositorInsertNon-targeting sgRNA3 (verified for knockout)
UseLentiviralTagsExpressionMutationPromoterEFS promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRha-ABE7.10-SpCas9-NG
Plasmid#170663PurposeExpresses the base editor ABE7.10-SpCas9-NG in bacterial cellsDepositorInsertABE7.10-SpCas9-NG
UseTags8xHIS and BP-NLSExpressionBacterialMutationPromoterpRhaBADAvailable sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTSK561
Plasmid#190816PurposeFor C-terminal -Halo Tagging of a gene of interest with URA3 selection marker in yeast S. cerevisiaeDepositorInsertHaloTag
UseBacterial cloning vectorTagsExpressionMutationPromoterAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pICE-NLS-mCherry
Plasmid#60364PurposePlasmid for expression of NLS-mCherry (control plasmid). Confers resistance to Puromycin. Expression is inducible in T-REx cells.DepositorInsertNLS-mCherry
UseTagsNLS from SV40 T antigen and mCherryExpressionMutationPromoterCMV-tetAvailable sinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUPD P35S (GB0552)
Plasmid#160554Purpose35s promoter for N-terminal fusionsDepositorInsert35s
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
LD101: pMVP (L3-L2) eCFP + WPRE
Plasmid#121775PurposepMVP L3-L2 entry plasmid, contains eCFP + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eCFP fusion to gene of interest in lentivirus vectors.DepositorInserteCFP + WPRE
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2 EGFP sgRNA2
Plasmid#164933PurposeExpresses EGFP sgRNA2 in mammalian cellsDepositorInsertsgRNA2 targeting Enhanced green fluorescent protein (verified for knockout)
UseLentiviralTagsExpressionMutationPromoterEFS promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
LLP617_αGCN4-mCherry
Plasmid#211785PurposeSunTag counterpart binding domain, aGCN4, fused to catalytically inert mCherry control, with GFP selectionDepositorInsertSnoopCatcher-DNMT3A
UseTags2xOLLASExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect DNMT3A…PromoterpEF1a and pSV40Available sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-trGluc
Plasmid#158959PurposeDNA donor plasmid containing repair template for Gaussia luciferase (Gluc) of bioluminescent repair reporter (BLRR).DepositorInserttrGluc
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-GST-EGFP
Plasmid#177136PurposeBacterial vector for expression of an N-terminal GST fusion of EGFP with a TEV protease site located between the GST tag and EGFPDepositorInsertEGFP
UseTagsExpressionBacterialMutationPromoterTacAvailable sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
JI401: Ad5 E1A/Hexon qRT-PCR standard
Plasmid#131753PurposePlasmid standard containing regions of the Ad5 E1A and Ad5 Hexon genes to create a qRT-PCR standard curve for detection of replication competent adenovirus (RCA).DepositorInsertsAd5 E1A gene fragment
Ad5 Hexon gene fragment
UseStandard for qrt-pcrTagsExpressionMutationPromoterN/AAvailable sinceOct. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
LLP783_SpyCatcher-DNMT3A-CO
Plasmid#211776PurposeSpyTag counterpart binding domain, SpyCatcher, fused to DNA methyltransferase DNMT3A, with GFP selectionDepositorInsertTraptavudin-DNMT3A
UseTags3xV5ExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect DNMT3A…PromoterpEF1a and pSV40Available sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only