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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,240 results for: spl

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Showing: 2441 - 2460 of 4240 results

  3. pX461-ABE8e-nSpCas9

    Plasmid
    #237460
    Purpose
    Transfection-based all-in-one base editor plasmid that expresses both adenine base editor (ABE8e-nSpCas9) and single guide RNAs (sgRNA) with BpiI cloning sites to clone sgRNA.
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Publication
    Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:
    Insert
    3xFlag_ABE8e_SpCas9(D10A)_6xNLS
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    chicken beta-actin promoter & U6
    Available since
    Jan. 12, 2026
    Availability
    Academic Institutions and Nonprofits only
    237460-plasmid-map-sequence-id-476159

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  4. pX461-evoCDA1-nSpCas9

    Plasmid
    #237461
    Purpose
    Transfection-based all-in-one base editor plasmid that expresses both cytosine base editor (evoCDA1-nSpCas9) and single guide RNAs (sgRNA) with BpiI cloning sites to clone sgRNA.
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Publication
    Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:
    Insert
    3xFlag_evoCDA1_ssDBD_nickase SpCas9 _6xUGI
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    chicken beta-actin promoter & U6
    Available since
    Jan. 12, 2026
    Availability
    Academic Institutions and Nonprofits only
    237461-plasmid-map-sequence-id-476160

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  7. pBOB-HA-Gnas

    Plasmid
    #245994
    Purpose
    Express HA N-terminal tagged mouse Galphas transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.
    Depositor
    Zhao Zhang
    Publication
    Xun et al Science. 2025 Jun 5;388(6751):eadp3989.
    Insert
    Gnas (Gnas Mouse)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    245994-plasmid-map-sequence-id-488657

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  8. pAGK112

    Plasmid
    #228127
    Purpose
    Express node 1531 retron with split retron ncRNA and gRNA to edit (SNIP + PAM recode) HEK4 gene in human cells
    Depositor
    Seth Shipman
    Publication
    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Retron node 1531 split retron ncRNA and gRNA to edit HEK4 gene (SNP + PAM recode) in human cells
    Expression
    Mammalian
    Promoter
    CAG for RT, U6/H1 for ncRNA/gRNA
    Available since
    Dec. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    228127-plasmid-map-sequence-id-455412

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  9. nCre-ST-NLS-P2A-NLS-pSC-cCre

    Plasmid
    #207638
    Purpose
    A plasmid encoding N-terminal Cre fused to SpyTag (ST) and photocaged SpyCatcher (pSC) fused to C-terminal Cre with a nuclear localization signal (NLS) sequence
    Depositor
    Cole DeForest
    Publication
    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1
    Insert
    nCre-SpyTag-NLS-P2A-NLS-photocaged SpyCatcher-cCre
    Expression
    Mammalian
    Mutation
    Amber stop codon at SpyCatcher’s critical lysine
    Promoter
    CMV
    Available since
    Oct. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    207638-plasmid-map-sequence-id-409880

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  12. mCherry-hPMCA2xb

    Plasmid
    #47751
    Purpose
    mammalian expression of mCherry tagged hPMCA2, xb splice variant
    Depositor
    Emanuel Strehler
    Publication
    Paszty et al Sci Signal. 2015 Feb 17;8(364):ra19. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PMCA2x/b (ATP2B2 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    xb splice variant
    Promoter
    CMV
    Available since
    Nov. 23, 2018
    Availability
    Academic Institutions and Nonprofits only

  15. pJH124

    Plasmid
    #48259
    Purpose
    Template plasmid for PCR-based transplant of Ashbya gossypii TEF1 promoter in yeast.
    Depositor
    Ronald Davis
    Publication
    Horecka et al Yeast. 2014 May;31(5):185-93. doi: 10.10
    Inserts
    URA3 3' fragment
    URA3 5' fragment
    Ag TEF1 promoter
    Use
    Routine cloning vector
    Mutation
    -242 upstream to URA3 ORF +495 and URA3 ORF from …
    Promoter
    Ag TEF1 promoter
    Available since
    Feb. 10, 2014
    Availability
    Academic Institutions and Nonprofits only
    48259-plasmid-map-sequence-id-69693

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  19. mCh-LgBiT-2A-EGFP-Vin-PILATeS-CT

    Plasmid
    #216763
    Purpose
    PiggyBac vector for stable co-expression of mCherry-LgBiT and EGFP-Vinculin-PILATeS-CT (700 pM) negative control in mammalian cells. Requires transposase
    Depositor
    Alex Dunn
    Publication
    Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry-LgBiT-P2A-T2A-EGFP-Vin-PILATeS-CT
    Tags
    EGFP and mCherry
    Expression
    Mammalian
    Available since
    Aug. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    216763-plasmid-map-sequence-id-428945

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Showing: 2441 - 2460 of 4240 results