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Showing: 4921 - 4960 of 5800 results

  9. pB80-KIF1A(1-383)-GFP-SSPB(milli)

    Plasmid
    #174626
    Purpose
    Constitutively active KIF1A for optogenetic heterodimerization to iLID via SSPB(milli)
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    KIF1A(1-383)-GFP-SSPB(milli) (Kif1a Mouse, Synthetic)
    Use
    Tags
    GFP-SSPB(milli)
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-383): Pro202Ala; EGFP: Met1Del; SSPB:…
    Promoter
    Chicken beta-actin
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    174626-plasmid-map-sequence-id-345348

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  19. pCEP4-HA-Flag-natT1R2 a22-end_I132S-S212N-D231G

    Plasmid
    #113953
    Purpose
    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R2 (TAS1R2 Human)
    Use
    Tags
    FLAG and Signal/leader sequence from influenza he…
    Expression
    Mammalian
    Mutation
    delete N-terminal 21 residues, I132S, S212N, and …
    Promoter
    cmv
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113953-plasmid-map-sequence-id-215167

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  22. pOD2087-trnDEG

    Plasmid
    #89369
    Purpose
    MosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pmec-18 (touch neuron specific).
    Depositor
    Karen Oegema
    Article
    Wang et al Development. 2017 Jun 15. pii: dev.15009
    Insert
    vhhGFP4-ZIF-1 (zif-1 Nematode, Synthetic)
    Use
    Targeting vector for mos1 transposon mediated sin…
    Tags
    vhhGFP4 (GFP nanobody)
    Expression
    Mutation
    Promoter
    Pmec-18
    Available since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    89369-plasmid-map-sequence-id-177235

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  30. pCR4-Dact1(1250-1601)

    Plasmid
    #52046
    Purpose
    To generate mRNA in situ probe against mouse Dact1 (Probe C in PMID 17013874). For antisense cut with Spe1, transcribe with T7. For sense cut with Not1, treat with T4 polymerase, transcribe with T3.
    Depositor
    Ben Cheyette
    Article
    Suriben et al Dev Dyn. 2006 Nov;235(11):3177-83.
    Insert
    Dact1(1250-1601) (Dact1 Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 18, 2014
    Availability
    Academic Institutions and Nonprofits only

  37. pAR69_STRI_m(KS_MAT)_H8_pET22b

    Plasmid
    #122849
    Purpose
    Expresses the condensing part of murine type I fatty acid synthase (FASN) consisting of the KS and MAT domain in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.
    Depositor
    Martin Grininger
    Article
    Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi
    Insert
    m(KS_MAT) (Fasn Mouse)
    Use
    Tags
    His8 tag and Twin-Strep tag
    Expression
    Bacterial
    Mutation
    wildtype
    Promoter
    T7 promoter
    Available since
    March 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    122849-plasmid-map-sequence-id-238542

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  40. pCDNA3.1-nAChr-beta3

    Plasmid
    #48811
    Purpose
    This report is the first to directly measure nAChR subunit stoichiometry using FRET and plasma membrane localization of α6- and β3-containing receptors using TIRF.
    Depositor
    Jerry Stitzel, Henry Lester
    Article
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Insert
    nAChr Beta 3 subunit, isoform 1 (Chrnb3 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    *
    Promoter
    CMV
    Available since
    Sept. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    48811-plasmid-map-sequence-id-193963

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Showing: 4921 - 4960 of 5800 results