We narrowed to 7,591 results for: Lif;

1. pMIA3-Olônne

   Plasmid

   #214447

   Purpose

   Expresses eSpCas9 and gRNA targeting Olônne-18 GSH

   Depositor

   Roger Foo

   Article

   Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

   Insert

   eSpCas9-P2A-mRuby2-P2A-tp53dn
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMIA10.53-Clover

   Plasmid

   #214450

   Purpose

   BxbI landing-pad mClover-IRES-puro donor. Use with BxbI expressing plasmid to swap payload into 4.721 landing-pad. Generates constitutively expressing cell line.

   Depositor

   Roger Foo

   Article

   Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

   Insert

   mClover
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Nrp1-Fc-His

   Plasmid

   #72097

   Purpose

   Expresses the extracellular region of the Neuropilin 1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Nrp1 (Nrp1 Mouse)
   

   Use

   Tags

   Fc-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. Sema7a-Fc-His

   Plasmid

   #72175

   Purpose

   Expresses the extracellular region of the Sema7A protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Sema7a (Sema7a Mouse)
   

   Use

   Tags

   Fc-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHalo-MyosinIIA-C25

   Plasmid

   #128573

   Purpose

   Studies of cellular contractility

   Depositor

   Dylan Burnette

   Article

   Fenix et al Elife. 2018 Dec 12;7. pii: 42144. doi: 1

   Insert

   Myosin IIA (Myh9 Mouse)
   

   Use

   Tags

   Halo

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBeta-lacZ-attBx2

   Plasmid

   #124445

   Purpose

   Plasmid for in vivo enhancer reporter assay. Plasmid contains a β-globin minimal promoter followed by a lacZ reporter gene derived from pRS16, with the entire reporter cassette flanked by attB sites.

   Depositor

   Yingguang Frank Chan

   Article

   Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

   Type

   Empty backbone

   Use

   Enhancer reporter assay construct

   Tags

   LacZ

   Expression

   Mammalian

   Mutation

   Promoter

   beta globin minimal promoter

   Available since

   May 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. Nrp2.3-AP-His

   Plasmid

   #71974

   Purpose

   Expresses the extracellular region of the Neuropilin 2, isoform 3 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Nrp2.3 (Nrp2 Mouse)
   

   Use

   Tags

   AP-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV HA hRB delta CDK + S612

   Plasmid

   #58914

   Purpose

   mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S612 restored

   Depositor

   Steven Dowdy

   Article

   Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

   Insert

   Rb (RB1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   delta CDK* with S612 restored

   Promoter

   CMV

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pCMV HA hRB delta CDK + S608

   Plasmid

   #58913

   Purpose

   mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S608 restored

   Depositor

   Steven Dowdy

   Article

   Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

   Insert

   Rb (RB1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   delta CDK* with S608 restored

   Promoter

   CMV

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

10. SspB-HaloTag-DHPH-TIAM1

    Plasmid

    #176114

    Purpose

    Heterodimerization with iLID, Rac1 activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    TIAM1 (TIAM1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Fireworks RFP[PTC+] (AVA2626)

    Plasmid

    #85444

    Purpose

    Expresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a PTC-containing beta-globin reporter.

    Depositor

    Joan Steitz

    Article

    Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:

    Insert

    tDNA1 EF1alfa-5xtdTomato-TEVprotease-PEST-beta-globin(dI1)(PTC39)-BGH polyA tDNA2 FRT-HygromycinR
    

    Use

    Minimal backbone fragment for low-copy bacterial …

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNS38-SadCas9-SNAP

    Plasmid

    #113718

    Purpose

    Bacterial vector for expression of Snap-tagged Staphylococcus aureus dCas9

    Depositor

    Gerald Schwank

    Article

    Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    HA-2xNLS-SNAP-NLS and His6-MBP-TEV

    Expression

    Bacterial

    Mutation

    D10A, N580A

    Promoter

    Available since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pScarlessHD-C-3xVHH05-DsRed

    Plasmid

    #171580

    Purpose

    For scarless insertion of 3xVHH05 NanoTag into C-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

    Depositor

    Hidde Ploegh, Norbert Perrimon

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    3xVHH05 (UBE2J1 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMRX-IZU-LAMP1-mRuby3

    Plasmid

    #222586

    Purpose

    Stable expression of LAMP1-mRuby3 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    LAMP1 (LAMP1 Human)
    

    Use

    Retroviral

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pmCherry C1 MFF

    Plasmid

    #157760

    Purpose

    Expression of mcherry:MFF in cells

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    MFF (MFF Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pScarlessHD-C-3x127D01-DsRed

    Plasmid

    #171578

    Purpose

    For scarless insertion of 3x127D01 NanoTag into C-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

    Depositor

    Hidde Ploegh, Norbert Perrimon

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    3x127D01 (CXCR2 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. CSII-PPM1H-mApple

    Plasmid

    #198473

    Purpose

    Mammalian expression of PPM1H-mApple

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    PPM1H-mApple (PPM1H Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Plxna2-AP-His

    Plasmid

    #71997

    Purpose

    Expresses the extracellular region of the PlexinA2 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Plxna2 (Plxna2 Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Nrp2.1-Fc-His

    Plasmid

    #72098

    Purpose

    Expresses the extracellular region of the Neuropilin 2, isoform 1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Nrp2.1 (Nrp2 Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLKO.1-shQKI-3371

    Plasmid

    #115455

    Purpose

    Constitutive lentiviral expression

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    shQKI-3371 (QKI Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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