167,948 results

1. pRDA_052

   Plasmid

   #136474

   Purpose

   Lentiviral expression of AsCas12a gRNA

   Depositor

   John Doench

   Article

   DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

   Insert

   Puromycin resistance
   

   Use

   Lentiviral and Synthetic Biology

   Promoter

   EF1a

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. mU6-CR1-CRISPRiv2_hU6-CR3-NT-ctrl-guide_EF1a-PuroR-T2A-BFP

   Pooled Library

   #197348

   Purpose

   To allow for genome-wide genetic modifier screening, the library has a second fixed non-targeting control guide.

   Depositor

   Rebecca Voorhees, Jonathan Weissman

   Article

   Guna et al BMC Genomics. 2023 Oct 30;24(1):651. doi

   Species

   Homo sapiens

   Use

   Lentiviral

   Available Since

   May 25, 2023

   Availability

   Academic Institutions and Nonprofits only

3. pICH47742:: pRPS5a-PE2max-NC::tHSP

   Plasmid

   #227694

   Purpose

   Golden Gate level 1, position 2, PE2max-NC expression cassette for Arabidopsis.

   Depositor

   Jae-Yean Kim

   Article

   Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

   Insert

   PE2max-NC being driven by pRPS5a and terminated by tHSP
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   pRPS5a

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CAG-NLS-GFP (AAV PHP.eB)

   Viral Prep

   #104061-PHPeB

   Purpose

   Ready-to-use AAV PHP.eB particles produced from pAAV-CAG-NLS-GFP (#104061). In addition to the viral particles, you will also receive purified pAAV-CAG-NLS-GFP plasmid DNA. CAG-driven NLS-GFP expression. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Viviana Gradinaru

   Article

   Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

   Promoter

   CAG

   Tags

   GFP

   Available Since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

5. pXR003: CasRx gRNA cloning backbone

   Plasmid

   #109053

   Purpose

   hU6-driven expression of guide RNAs compatible with CasRx. 5' processed DR followed by BbsI sites for guide cloning.

   Depositor

   Patrick Hsu

   Article

   Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-pgk-Cre (AAV Retrograde)

   Viral Prep

   #24593-AAVrg

   Purpose

   Ready-to-use AAV Retrograde particles produced from AAV-pgk-Cre (#24593). In addition to the viral particles, you will also receive purified AAV-pgk-Cre plasmid DNA. Cre expression under the PGK promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons.

   Depositor

   Patrick Aebischer

   Promoter

   pgk

   Available Since

   July 24, 2017

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA C12 ecDHFR DD YFP HA

   Plasmid

   #192815

   Purpose

   Expresses an N-terminal enhanced destabilizing domain (DD) version of E. coli DHFR with higher basal turnover in mammalian systems. Contains missense mutations W74R/T113S/E120D/Q146L.

   Depositor

   John Hulleman

   Article

   Nakahara et al ACS Chem Biol. 2022 Sep 19. doi: 10.1021

   Insert

   E. coli dihydrofolate reductase
   

   Tags

   enhanced yellow fluorescent protein and hemagglut…

   Expression

   Mammalian

   Mutation

   W74R/T113S/E120D/Q146L

   Promoter

   CMV

   Available Since

   Nov. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CMV-FLEX-SaCas9-U6-sgSlc32a1

   Plasmid

   #159905

   Purpose

   Mutagenesis of Slc32a1

   Depositor

   Larry Zweifel

   Insert

   Slc32a1 (Slc32a1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. hCRISPRi-v2, Kinases, Phosphatases, and Drug Targets (h1), top 5 sgRNAs/gene

   Pooled Library

   #83971

   Purpose

   Human CRISPRi Pooled Library tartgeting kinsases, phosphatases and Drug Targets

   Depositor

   Jonathan Weissman

   Article

   Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

   Available Since

   Dec. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pBBR-Cas9ΔyqhD-HA500

    Plasmid

    #220369

    Purpose

    A single-plasmid-based, easily cured CRISPR/Cas9 system for genome engineering in Pseudomonas putida KT2440

    Depositor

    Yilan Liu

    Article

    Wen et al Microb Cell Fact. 2024 Dec 30;23(1):349.

    Inserts

    SpCas9
    sgRNA for yqhD in P. putida KT2440
    

    Use

    CRISPR; Broad host range

    Expression

    Bacterial

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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