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Showing: 2481 - 2500 of 6460 results

  4. pLP-PIGA (pLP140)

    Plasmid
    #168461
    Purpose
    Big-IN Landing Pad plasmid encoding pEF1a-driven mScarlet-P2A-CreERT2-P2A-PuorR-P2A-hmPIGA expression cassette flanked by heterotypic LoxM/LoxP sites and BsaI homology arms cloning sites.
    Depositor
    Jef Boeke, Matthew Maurano
    Article
    Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118
    Type
    Empty backbone
    Use
    Cre/Lox, Mouse Targeting, and Synthetic Biology ;…
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    168461-plasmid-map-sequence-id-362577

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  6. pUF1-dCas9-Sir2a

    Plasmid
    #122510
    Purpose
    Combining with the specific sgRNA sequence to repress the Plasmodium falciparum endogenous gene transcription, through the decreasing of H3K9 acetylation.
    Depositor
    Lubin Jiang
    Article
    Xiao et al Proc Natl Acad Sci U S A. 2019 Jan 2;116
    Insert
    dCas9-Sir2a
    Use
    CRISPR
    Tags
    3*FLAG
    Mutation
    dCas9(D10A,H840A)
    Promoter
    PfHsp86
    Available Since
    June 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    122510-plasmid-map-sequence-id-243592

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  7. W45A mutant of H3K9me3 biosensor

    Plasmid
    #120808
    Purpose
    FRET biosensor. Disrupting HP1 binding capability in H3K9me3 biosensor to abolish the detection capability
    Depositor
    Yingxiao Wang
    Article
    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p
    Insert
    Truncated YPet-HP1(W45A mutation)-EV linker-ECFP-mouse histone H3
    Expression
    Mammalian
    Mutation
    Tryptophan 45 is mutated to Alanine 45 on HP1 dom…
    Promoter
    CMV
    Available Since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    120808-plasmid-map-sequence-id-235136

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  8. AAEL006511-Cas9

    Plasmid
    #100580
    Purpose
    The promoter of AAEL006511 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti
    Depositor
    Omar Akbari
    Article
    Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p
    Inserts
    AAEL006511-hspcas9-GFP
    Opie2-dsRed
    Use
    CRISPR
    Tags
    GFP and dsRED
    Expression
    Insect
    Promoter
    AAEL006511 and Opie2
    Available Since
    Oct. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    100580-plasmid-map-sequence-id-193175

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  16. pSU313

    Plasmid
    #169695
    Purpose
    Template plasmid for generating C-terminal 1xFLAG epitope tag fusions to bacterial genes by "Lambda red" recombineering.
    Depositor
    Nara Figueroa
    Article
    Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98
    Insert
    1xFLAG epitope tag adjacent to FRT-flanked cat (CamR) gene
    Tags
    1xFLAG tag
    Expression
    Bacterial
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169695-plasmid-map-sequence-id-334516

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  17. pSU314

    Plasmid
    #169696
    Purpose
    Template plasmid for generating C-terminal hemagglutinin (1xHA) epitope tag fusions to bacterial genes by "Lambda red" recombineering.
    Depositor
    Nara Figueroa
    Article
    Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98
    Insert
    1xHA epitope tag adjacent to FRT-flanked cat (CamR) gene
    Tags
    1xHA tag
    Expression
    Bacterial
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169696-plasmid-map-sequence-id-334524

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Showing: 2481 - 2500 of 6460 results