We narrowed to 13,736 results for: TIM;

1. pCMV-T7-SpCas9-VRQR-P2A-mScarlet (MNW004)

   Plasmid

   #174139

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized SpCas9-VRQR with BPNLS-3xFLAG-P2A-mScarlet
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-mScarlet

   Expression

   Mammalian

   Mutation

   VRQR=D1135V/G1218R/R1335Q/T1337R

   Promoter

   CMV and T7

   Available since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBD21_035

   Plasmid

   #109152

   Purpose

   pL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of EGFP

   Depositor

   Ron Weiss

   Article

   Gam et al Nucleic Acids Res. 2019 Oct 10;47(18):e1

   Inserts

   mRFP
   EGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV and pTet

   Available since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. C453-E04: attB4-CMV51p>-attB5

   Plasmid

   #162973

   Purpose

   Gateway attB4/attB5 entry clone for mammalian expression using an optimized human CMV promoter

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   optimized human CMV promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. C413-E36: attB4-CMV51p>-attB1r

   Plasmid

   #162927

   Purpose

   Gateway attB4/attB1r entry clone for mammalian expression using an optimized human CMV promoter

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   optimized CMV promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFapO_94

   Plasmid

   #149597

   Purpose

   pXI-3-LoxP-KlURA3-pTEF1- FapO_94-yeGFP

   Depositor

   Michael Jensen

   Article

   Ambri et al ACS Synth Biol. 2020 Feb 21;9(2):218-226

   Insert

   yeGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1-FapO_94

   Available since

   Dec. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pXylO_94

   Plasmid

   #149598

   Purpose

   pXI - 3 -LoxP -KlURA3 -pTEF1 - XylO_94 -yeGFP

   Depositor

   Michael Jensen

   Article

   Ambri et al ACS Synth Biol. 2020 Feb 21;9(2):218-226

   Insert

   yeGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1-XylO_94

   Available since

   Dec. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTetO_105

   Plasmid

   #149599

   Purpose

   pXI - 3 -LoxP -KlURA3 -pTEF1 - TetO_105 -yeGFP

   Depositor

   Michael Jensen

   Article

   Ambri et al ACS Synth Biol. 2020 Feb 21;9(2):218-226

   Insert

   yeGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1-TetO_105

   Available since

   Dec. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFA271

   Plasmid

   #149600

   Purpose

   (parent plasmid pCFB262) pXII - 4 -LoxP -SpHIS5 -209bp - pCYC1_BenO_183 -yeGFP

   Depositor

   Michael Jensen

   Article

   Ambri et al ACS Synth Biol. 2020 Feb 21;9(2):218-226

   Insert

   yeGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFA272

   Plasmid

   #149601

   Purpose

   (parent plasmid pCFB262) pXII - 4 -LoxP -SpHIS5 -209bp - pCYC1_BenO_182 -yeGFP

   Depositor

   Michael Jensen

   Article

   Ambri et al ACS Synth Biol. 2020 Feb 21;9(2):218-226

   Insert

   yeGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFA197

    Plasmid

    #149602

    Purpose

    (parent plasmid pCFB262) pXII - 4 -LoxP -SpHIS5 -209bp - pCYC1 -PcaO -108 -yeGFP

    Depositor

    Michael Jensen

    Article

    Ambri et al ACS Synth Biol. 2020 Feb 21;9(2):218-226

    Insert

    yeGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PcaO_1O8

    Available since

    Dec. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pVD_79

    Plasmid

    #149603

    Purpose

    (parent plasmid pCFB257) pX - 3 -LoxP -KlLEU2 -pTEF1 - TetR

    Depositor

    Michael Jensen

    Article

    Ambri et al ACS Synth Biol. 2020 Feb 21;9(2):218-226

    Insert

    TetR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Dec. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pVD_01

    Plasmid

    #149606

    Purpose

    pTEF1-VanR inserted in pRS416 backbone

    Depositor

    Michael Jensen

    Article

    Ambri et al ACS Synth Biol. 2020 Feb 21;9(2):218-226

    Insert

    VanR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJ414 - DANCER-2

    Plasmid

    #136386

    Purpose

    Expresses DANCER-2 dynamic protein

    Depositor

    Roberto Chica

    Article

    Davey et al Nat Chem Biol. 2017 Dec;13(12):1280-1285

    Insert

    DANCER-2
    

    Use

    Tags

    6x-His tag (N-term of insert)

    Expression

    Bacterial

    Mutation

    Mutation from GB1 WT: Y3F-L5A-L7I-A34F-V39L

    Promoter

    T7 promoter

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJ414 - NERD-S

    Plasmid

    #136388

    Purpose

    Expresses NERD-S static protein

    Depositor

    Roberto Chica

    Article

    Davey et al Nat Chem Biol. 2017 Dec;13(12):1280-1285

    Insert

    NERD-S
    

    Use

    Tags

    6x-His tag (N-term of insert)

    Expression

    Bacterial

    Mutation

    Mutation from GB1 WT: Y3F-L7I-A34F-V39I-V54I

    Promoter

    T7 promoter

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJ414 - NERD-C

    Plasmid

    #136389

    Purpose

    Expresses NERD-C static protein

    Depositor

    Roberto Chica

    Article

    Davey et al Nat Chem Biol. 2017 Dec;13(12):1280-1285

    Insert

    NERD-C
    

    Use

    Tags

    6x-His tag (N-term of insert)

    Expression

    Bacterial

    Mutation

    Mutation from GB1 WT: Y3F-L7I-F30L-V39I

    Promoter

    T7 promoter

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKM45

    Plasmid

    #154152

    Purpose

    Firefly luciferase with no promoter for negative control for luciferase assays

    Depositor

    Kevin Maringer

    Article

    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

    Insert

    Firefly luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYFP-lacY-10

    Plasmid

    #140139

    Purpose

    Evaluating the impact of feedback and high sensor concentration on information transmission of a genetic sensor.

    Depositor

    Drew Tack

    Article

    Sarkar et al Commun Biol. 2020 Apr 30;3(1):203. doi:

    Insert

    lacY (lacY )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. FoxFTVC+bpFOG_Cdkn1b.a

    Plasmid

    #107523

    Purpose

    Negative regulator of cell cycle progression by G1/S transition inhibition

    Depositor

    Lionel Christiaen

    Article

    Razy-Krajka et al Elife. 2018 Feb 6;7. pii: 29656. doi: 10

    Insert

    Cdkn1b.a
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    TVC-specific FoxF enhancer (FoxFTVC+bpFOG)

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. FoxFTVC+bpFOG_Cdkn1b.b

    Plasmid

    #107524

    Purpose

    Negative regulator of cell cycle progression by G1/S transition inhibition

    Depositor

    Lionel Christiaen

    Article

    Razy-Krajka et al Elife. 2018 Feb 6;7. pii: 29656. doi: 10

    Insert

    Cdkn1b.b
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    TVC-specific FoxF enhancer (FoxFTVC+bpFOG)

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGuide-P1-O1a (EL702)

    Plasmid

    #140040

    Purpose

    CRISPR DuMPLING negative control plasmid with probe/barcode P1 and negative control lacO1array spacer in the sgRNA. Also template for library PCRs.

    Depositor

    Johan Elf

    Article

    Camsund et al Nat Methods. 2020 Jan;17(1):86-92. doi:

    Insert

    barcode P1 and sgRNA lacO1 array
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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