We narrowed to 11,219 results for: NSI

1. pAB082g

   Plasmid

   #79239

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   Ppro1 rpoZ-Cry1Ac-d23B
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ppro1

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAB094b

   Plasmid

   #79242

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PBAD rpoZ-HA4 (Y35A); PC araC
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PBAD; PC

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAB094c

   Plasmid

   #79243

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PBAD rpoZ-HA4 (R38A); PC araC
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PBAD; PC

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAB094d

   Plasmid

   #79244

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PBAD rpoZ-HA4 (E52A); PC araC
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PBAD; PC

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBABE-hygro-mTurquoise2-Nuf2

   Plasmid

   #80762

   Purpose

   Express Nuf2 N-terminally labeled with mTurquoise2

   Depositor

   Daniel Needleman

   Article

   Yoo et al Elife. 2018 Jul 25;7. pii: 36392. doi: 1

   Insert

   Nuf2 (NUF2 Human)
   

   Use

   Retroviral

   Tags

   mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. NaVSp1 Ae1 CC pIRES

   Plasmid

   #128079

   Purpose

   Mammalian cell expression vector for (NaVSp1 1-236 NaVAe1 265-288) chimera bacterial sodium channel.

   Depositor

   Dan Minor

   Article

   Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

   Insert

   NaV Sp1 and NaVAe1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. NaVSp1 Bh1 CTD pIRES

   Plasmid

   #128080

   Purpose

   Mammalian cell expression vector for (NaVSp1 1-220 NaVBh1 236-274) chimera bacterial sodium channel.

   Depositor

   Dan Minor

   Article

   Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

   Insert

   NavSp1_Bh1CTD
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. NaVSp1 Ms CTD pIRES

   Plasmid

   #128081

   Purpose

   Mammalian cell expression vector for (NaVSp1 1-220 NaVMs 222-274) chimera bacterial sodium channel.

   Depositor

   Dan Minor

   Article

   Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

   Insert

   NavSp1_MsCTD
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. NaVSp1 Pz CTD pIRES

   Plasmid

   #128083

   Purpose

   Mammalian cell expression vector for (NaVSp1 1-220 NaVPz 224-262) chimera bacterial sodium channel.

   Depositor

   Dan Minor

   Article

   Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

   Insert

   NavSp1_PzCTD
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. NaVAe1 Sp1 CTD pIRES

    Plasmid

    #128085

    Purpose

    Mammalian cell expression vector for (NaVAe1 1-243 NaVSp1 220-255) chimera bacterial sodium channel.

    Depositor

    Dan Minor

    Article

    Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

    Insert

    NaVAe1 and Sp1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRK5_tAKARδ

    Plasmid

    #119916

    Purpose

    Mammalian expression of the tAKARδ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

    Depositor

    Haining Zhong

    Article

    Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

    Insert

    tAKARδ
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRK5_tAKARε

    Plasmid

    #119917

    Purpose

    Mammalian expression of the tAKARε sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

    Depositor

    Haining Zhong

    Article

    Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

    Insert

    tAKARε
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLIB-HISX6-TEV-RSP4

    Plasmid

    #176693

    Purpose

    Expression of Chlamydomonas reinhardtii RSP4 recombinant protein in insect cells

    Depositor

    Ron Vale

    Article

    Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

    Insert

    RSP4
    

    Use

    Tags

    HISX6

    Expression

    Insect

    Mutation

    None

    Promoter

    polyhedrin

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLIB-RSP5

    Plasmid

    #176694

    Purpose

    Expression of Chlamydomonas reinhardtii RSP5 recombinant protein in insect cells

    Depositor

    Ron Vale

    Article

    Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

    Insert

    RSP5 (RSP5 Chlamydomonas reinhardtii)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    None

    Promoter

    polyhedrin

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBR9-genomic_RSP6-HAX3

    Plasmid

    #176703

    Purpose

    Rescue of RSP6 Chlamydomonas mutant with tagged RSP6

    Depositor

    Ron Vale

    Article

    Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

    Insert

    RSP6
    

    Use

    Tags

    HAX3

    Expression

    Plant

    Mutation

    None

    Promoter

    rbcs2

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MG1655-tMMR

    Bacterial Strain

    #62392

    Purpose

    E. coli MG1655 temperature-sensitive mismatch repair deficient strain, harboring mutS(A134V) mutL(G62S), and nadA::Tn10 pglΔ8 [λ cI857 Δ(cro-bioA)]} for λ Red expression

    Depositor

    Csaba Pál

    Article

    Nyerges et al Nucleic Acids Res. 2014 Apr;42(8):e62. d

    Bacterial Resistance

    Tetracycline

    Species

    Escherichia coli

    Available since

    March 4, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pINDUCER21-puro_Gateway-3xFLAG

    Plasmid

    #172981

    Purpose

    Gateway Destination vector for doxycycline-inducible expression of ORF

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDuet TDP43 WT

    Plasmid

    #27462

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Coexpression

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 31, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FUGW-ER-TurboID

    Plasmid

    #200965

    Purpose

    3rd gen lentiviral plasmid for expressing endoplasmic reticulum localized TurboID with EGFP

    Depositor

    Keriann Backus

    Article

    Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

    Insert

    ER-TurboID
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-sapphireCKAR

    Plasmid

    #118468

    Purpose

    cp-T-sapphire-based single-color biosensor for monitoring Protein Kinase C activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    sapphireCKAR
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3-sapphireCKAR(T/A)

    Plasmid

    #118469

    Purpose

    Negative-control mutant for sapphireCKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    sapphireCKAR(T/A)
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3.1(+)-blueAKAR(T/A)

    Plasmid

    #118471

    Purpose

    Negative-control mutant for blueAKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    blueAKAR(T/A)
    

    Use

    Tags

    6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGEX-KG-D4H*-mCherry

    Plasmid

    #134604

    Purpose

    fluorescent cholesterol-binding probe

    Depositor

    Roberto Zoncu

    Article

    Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

    Insert

    D4H*
    

    Use

    Tags

    GST and mCherry

    Expression

    Bacterial

    Mutation

    Y415A and A463W

    Promoter

    Available since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Venus-rab11a

    Plasmid

    #234744

    Purpose

    Mammalian expression of recycling endosome marker Venus-rab11a

    Depositor

    Nevin Lambert

    Article

    Lan et al Traffic. 2012 Nov;13(11):1450-6. doi: 10

    Insert

    Venus-rab11a (RAB11A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Venus-rab7a

    Plasmid

    #234743

    Purpose

    Mammalian expression of late endosome marker Venus-rab7a

    Depositor

    Nevin Lambert

    Article

    Lan et al Traffic. 2012 Nov;13(11):1450-6. doi: 10

    Insert

    Venus-rab7a (Rab7 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGGAselect

    Plasmid

    #195714

    Purpose

    pGGAselect is a cloning vector for Golden Gate Assembly using BsaI, BsmBI, and BbsI Type IIS restriction enzymes.

    Depositor

    Eric Cantor

    Article

    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. UPB-stomach (UPB1)

    Plasmid

    #180784

    Purpose

    Unique Projection Barcode (UPB1)-Stomach for Projection-seq

    Depositor

    Rui Chang

    Article

    Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

    Insert

    humanized ChR2-H134R (partial)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. UPB-lung (UPB2)

    Plasmid

    #180788

    Purpose

    Unique Projection Barcode (UPB2)-Lung for Projection-seq

    Depositor

    Rui Chang

    Article

    Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

    Insert

    humanized ChR2-H134R (partial)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. UPB-colon (UPB4)

    Plasmid

    #180786

    Purpose

    Unique Projection Barcode (UPB4)-Colon for Projection-seq

    Depositor

    Rui Chang

    Article

    Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

    Insert

    hM3Dq ( partial )
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pACE-GIRK4-mC-S

    Plasmid

    #172427

    Purpose

    Expression of full-length, human GIRK4 with C-terminal mCherry StrepTag II

    Depositor

    Arthur Laganowsky

    Article

    Qiao et al Biochemistry. 2021 Dec 21;60(50):3813-38

    Insert

    GIRK4 (KCNJ5 Human)
    

    Use

    Tags

    mCherry Streptag II

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3-ExRai-AMPKAR

    Plasmid

    #192446

    Purpose

    Genetically encoded excitation-ratiometric fluorescent biosensor for monitoring AMPK activity in living cells.

    Depositor

    Jin Zhang

    Article

    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

    Insert

    ExRai-AMPKAR
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. HA-Foxo1 (pCMV5)

    Plasmid

    #12142

    Depositor

    Domenico Accili

    Article

    Nakae et al J Clin Invest. 2001 Nov . 108(9):1359-67

    Insert

    Foxo1 (Foxo1 Mouse)
    

    Use

    Tags

    HA and Myc

    Expression

    Mammalian

    Mutation

    Wild-type

    Promoter

    CMV

    Available since

    Aug. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3-Mito-ExRai-AMPKAR

    Plasmid

    #192448

    Purpose

    ExRai-AMPKAR targeted to the mitochondrial outer membrane.

    Depositor

    Jin Zhang

    Article

    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

    Insert

    Mito-ExRai-AMPKAR
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA_RGG-GFP-RGG

    Plasmid

    #124930

    Purpose

    Expression of RGG-GFP-RGG

    Depositor

    Daniel Hammer, Matthew Good, Benjamin Schuster

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3-PM-ExRai-CKAR2

    Plasmid

    #236098

    Purpose

    Plasma membrane-targeted enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells.

    Depositor

    Jin Zhang

    Article

    Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038

    Insert

    Lyn-ExRai-CKAR2
    

    Use

    Tags

    N-terminal targeting sequence from Lyn kinase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3-Lyso-ExRai-AMPKAR

    Plasmid

    #192449

    Purpose

    ExRai-AMPKAR targeted to the lysosomal membrane.

    Depositor

    Jin Zhang

    Article

    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

    Insert

    Lyso-ExRai-AMPKAR
    

    Use

    Tags

    Full-length LAMP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAB076i3

    Plasmid

    #79198

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI-SH2ABL1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PlacZ-opt (OR1); Ppro1

    Available since

    April 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMC-6-BeYDV-Rep-RepA-9

    Plasmid

    #197715

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    BeYDV Rep/RepA proteins: native ORFs
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAB076i7

    Plasmid

    #79201

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI(RR69)-SH2ABL1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PlacZ-opt (OR1); Ppro1

    Available since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. GFP-C1-PLCdelta-PH

    Plasmid

    #21179

    Purpose

    encodes a PI(4,5)P2 lipid selective PH domain that can be used as a fluorescent translocation biosensor to monitor changes or local differences in the concentration of plasma membrane PI(4,5)P2 lipids

    Depositor

    Tobias Meyer

    Article

    Stauffer et al Curr Biol. 1998 Mar 12. 8(6):343-6.

    Insert

    PLCdelta (PLCD1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    PH domain of human PLC delta, amino acids Met1–Il…

    Promoter

    Available since

    July 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge