We narrowed to 6,005 results for: 338
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Plasmid#159499PurposeTests for the impacts 1 uracil in a poly-uracil tract in conjunctiuon with an immediately upstream hairpin have on human polymerase III transcription elongation from a U6 promoter.DepositorInsertTermination Module:Linear-2_PolyU(1)_HairpinDistance(0)nts
UseTagsExpressionMammalianMutationPromoterU6-27Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Linear-1_PolyU(4)_HairpinDistance(10)nts_Tornado-Corn
Plasmid#159498PurposeTests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter.DepositorInsertTermination Module:Linear-1_PolyU(4)_HairpinDistance(10)nts
UseTagsExpressionMammalianMutationPromoterU6-27Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Linear-1_PolyU(4) _HairpinDistance(0)_Tornado-Corn
Plasmid#159496PurposeTests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with an immediately upstream hairpin have on human polymerase III transcription elongation from a U6 promoter.DepositorInsertTermination Module:Linear-1_PolyU(4) _HairpinDistance(0)
UseTagsExpressionMammalianMutationPromoterU6-27Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Linear-1_PolyU(1)_HairpinDistance(0)nts_Tornado-Corn
Plasmid#159495PurposeTests for the impacts 1 uracil in a poly-uracil tract in conjunctiuon with an immediately upstream hairpin have on human polymerase III transcription elongation from a U6 promoter.DepositorInsertTermination Module:Linear-1_PolyU(1)_HairpinDistance(0)nts
UseTagsExpressionMammalianMutationPromoterU6-27Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265H
Plasmid#149554PurposeGateway entry clone for CRISPR-iSpyMacCas9 hAID C-T base editing at NAAR PAMsDepositorInserthAID-ziSpyMacCas9(D10A)-UGI
UseCRISPRTagsExpressionPlantMutationD10A, R221K, N394K, Protospacer interacting domai…PromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ266B
Plasmid#149555PurposeGateway entry clone for CRISPR-iSpyMacCas9 PmCDA1 C-T base editing at NAAR PAMsDepositorInsertziSpyMacCas9(D10A)-PmCDA1-UGI
UseCRISPRTagsExpressionPlantMutationD10A, R221K, N394K, Protospacer interacting domai…PromoterAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBz172_CsoS2_N1_GFP
Plasmid#140852PurposeHis-tagged first N-peptide of NTD fused to Superfolder GFP ([N1]-GFP) used for BLI and MST, pET-14b backboneDepositorInsertCsoS2 NTD repeat 1 fused to GFP
UseTagsHexahistidine tag, Strep-Tag II, and Superfolder …ExpressionBacterialMutationPromoterT7Available sinceJune 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBz175_CsoS2_N4_GFP
Plasmid#140855PurposeHis-tagged fourth N-peptide of NTD fused to Superfolder GFP ([N4]-GFP) used for BLI, pET-14b backboneDepositorInsertCsoS2 NTD repeat 4 fused to GFP
UseTagsHexahistidine tag, Strep-Tag II, and Superfolder …ExpressionBacterialMutationPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBz173_CsoS2_N2_GFP
Plasmid#140853PurposeHis-tagged second N-peptide of NTD fused to Superfolder GFP ([N2]-GFP) used for BLI, pET-14b backboneDepositorInsertCsoS2 NTD repeat 2 fused to GFP
UseTagsHexahistidine tag, Strep-Tag II, and Superfolder …ExpressionBacterialMutationPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBz174_CsoS2_N3_GFP
Plasmid#140854PurposeHis-tagged third N-peptide of NTD fused to Superfolder GFP ([N3]-GFP) used for BLI, pET-14b backboneDepositorInsertCsoS2 NTD repeat 3 fused to GFP
UseTagsHexahistidine tag, Strep-Tag II, and Superfolder …ExpressionBacterialMutationPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLz26_CsoS2_N1-N2_GFP
Plasmid#140851PurposeHis-tagged first two N-peptides of NTD fused to Superfolder GFP ([N1-N2]-GFP) used for MST, pET-14b backboneDepositorInsertCsoS2 NTD repeats 1-2 fused to GFP
UseTagsHexahistidine tag, Strep-Tag II, and Superfolder …ExpressionBacterialMutationPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCool-human TSC1 (939-992)
Plasmid#132618Purposeexpresses human TSC1 (939-992) in E. coliDepositorInsertTSC1 (939-992)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
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Geph UTR3m shRNA
Plasmid#121071PurposeNegative control shRNA for plasmid 121070: shRNA targeting the 3'-untranslated region (UTR) of the gephyrin mRNADepositorInsertGPHN shRNA (Gphn Rat)
UseRNAiTagsExpressionMutationPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
PX458_HBP1_1
Plasmid#86320PurposeEncodes gRNA for 3' target of human HBP1DepositorInsertgRNA against HBP1 (HBP1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_HBP1
Plasmid#86272PurposeDonor vector for 3' FLAG tag of human HBP1DepositorInsertHBP1 homology arms (HBP1 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
FUP95Q15AGW (D5)
Plasmid#74028Purposelentiviral expression of Psd95-Q15A-EGFP fusionDepositorInsertPsd95 (DLG4 Rat)
UseLentiviralTagsExpressionMammalianMutationQ15APromoterhUbCAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
FUP95E17RGW (D6)
Plasmid#74029Purposelentiviral expression of Psd95-E17R-EGFP fusionDepositorInsertPsd95 (DLG4 Rat)
UseLentiviralTagsExpressionMammalianMutationE17RPromoterhUbCAvailable sinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
FUP95DSKGW (C8)
Plasmid#74024Purposelentiviral expression of Psd95-delta SK-EGFP fusionDepositorInsertPsd95 (DLG4 Rat)
UseLentiviralTagsExpressionMammalianMutationdelta SKPromoterhUbCAvailable sinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS-flt4um159-HA
Plasmid#67704PurposeExpression constructDepositorInsertflt4 (flt4 Zebrafish)
UseTagsHAExpressionMammalianMutationum159PromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only