We narrowed to 7,747 results for: Trac;

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TIA1 RRM-CFP-FRBx5

Plasmid

#103780

Purpose

expresses iPOLYMER component in mammalian cells

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

TIA1 RRM-CFP-FRBx5

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pSK234

Plasmid

#131149

Purpose

dual yTRAP, Gateway destination vector of aTF2 fusion reporting on mKate2

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Type

Empty backbone

Use

Gateway destination vector

Expression

Yeast

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pSK247

Plasmid

#131148

Purpose

dual yTRAP, Gateway destination vector of aTF2 fusion reporting on mNeonGreen

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Type

Empty backbone

Expression

Yeast

Promoter

pSUP35, min pCYC1

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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tdEos-Vimentin-N-18

Plasmid

#57690

Purpose

Localization: Intermediate Filaments, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

NM_003380.3 (VIM Human)

Tags

tdEos

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pMM2-hPMCA2x/b

Plasmid

#47582

Depositor

Emanuel Strehler

Article

Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

Insert

PMCA2x/b (ATP2B2 Human)

Expression

Mammalian

Promoter

SV40

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1 AT1AR-324delta

Plasmid

#45635

Depositor

Robert Lefkowitz

Article

Wei et al J Biol Chem. 2004 Nov 12;279(46):48255-6

Insert

AT1AR-324delta (Agtr1a Rat)

Tags

HA

Expression

Mammalian

Mutation

added stop codon at 324 aa

Promoter

CMV

Available Since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only
pSK226

Plasmid

#129268

Purpose

dual yTRAP, RNQ1 fusion to aTF2 reporting on [RNQ+] state with mNeonGreen reporter

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Insert

Rnq1

Expression

Yeast

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pSEM386 - mosTI unc-119 - NNN - gfp - tbb-2

Plasmid

#182345

Purpose

GFP fusion cloning vector for mosTI(unc-119). Transgene inserted upstream of gfp and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available Since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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secCitrine

Plasmid

#181964

Purpose

Basic chitinase signal peptide fused to Citrine, under control of MUM4_1.5kb promoter

Depositor

George Haughn

Article

Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

Insert

signal peptide sequence from basic chitinase

Tags

Citrine YFP

Expression

Plant

Promoter

1.5 kb MUM4 promoter

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCFJ2241 - [1-2] ENTR - Fluor - ce-mCardinal (1 intron, syntrons(3), no_atg, no_stop)

Plasmid

#159860

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-mCardinal (1 intron, syntrons(3), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSwith-tdTomato

Plasmid

#60953

Purpose

Cre recombinase-mediated cassette exchange

Depositor

Liangxue Lai

Article

Li et al Cell Res. 2014 Apr;24(4):501-4. doi: 10.

Insert

tdTomato

Use

Cre/Lox

Available Since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pIW086

Plasmid

#134132

Purpose

Bacterial expression of His-TEV-Ska1 and Ska2

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

His-TEV-human Ska1 ; human Ska2

Tags

His-TEV

Expression

Bacterial

Mutation

K183A K184A

Available Since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pMDJ15 - pEXP[Peft-3 | mCardinal (NLS) | tbb-2 UTR]

Plasmid

#159801

Purpose

Expression construct with ubiquitous expression to test fluorescence level on microscope

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Peft-3 | mCardinal (NLS) | tbb-2 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSEM389 - mosTI unc-119 - NNN - mScarlet(nls) - tbb-2

Plasmid

#182349

Purpose

mScarlet transcriptional reporter cloning vector for mosTI(unc-119). Transgene inserted upstream of mScarlet(2xNLS) and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available Since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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pET-28a-His6-SUMO-VAMP8

Plasmid

#135553

Purpose

Express mouse VAMP8 in E. coli BL21, purified via HisPur Ni-NTA Resin

Depositor

Jingshi Shen

Article

Yu et al Methods Mol Biol. 2019;1860:237-249. doi

Insert

VAMP8

Tags

6xHis tag, thrombin recognition and cleavage site…

Expression

Bacterial

Mutation

No ATG start codon

Promoter

T7 promoter

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pQUAST>stop>shibire (ts1)

Plasmid

#24363

Depositor

Liqun Luo

Article

Potter et al Cell. 2010 Apr 30. 141(3):536-48.

Insert

Shibire (ts1) (shi Fly)

Expression

Insect

Available Since

April 30, 2010

Availability

Academic Institutions and Nonprofits only

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gRD21A-mRFP

Plasmid

#181959

Purpose

RD21A protein tagged with mRFP, under control of native promoter

Depositor

George Haughn

Article

Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

Insert

RD21A genomic sequence including 2kb upstream of the ATG

Tags

mRFP

Expression

Plant

Promoter

RD21A native

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCFJ2210 - [1-2] ENTR - Fluor - mMaple3(1 intron, syntrons(3), no_start, no_stop)

Plasmid

#159864

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - mMaple3(1 intron, syntrons(3), no_start, no_stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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CD4_LD 0_CS G_tTA-BFP

Plasmid

#58869

Purpose

MESA target chain with CD4 ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion

Depositor

Joshua Leonard

Article

Daringer et al ACS Synth Biol. 2014 Mar 11.

Insert

MESA target chain with CD4 ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence

Use

AAV

Tags

EBFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pMDJ16 - pEXP[Peft-3 | mMaple3 (NLS) | tbb-2 UTR]

Plasmid

#159802

Purpose

Expression construct with ubiquitous expression to test fluorescence level on microscope

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Peft-3 | mMaple3 (NLS) | tbb-2 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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