We narrowed to 6,485 results for: plasmid dna

1. pLPC52

   Plasmid

   #209991

   Purpose

   Contains Level 0 Part: 5' Connector (5C2SN) for the construction of Level 1 plasmids

   Depositor

   Georg Fritz, Thorsten Mascher

   Article

   Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

   Insert

   5' Connector (5C2SN)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLPC51

   Plasmid

   #209990

   Purpose

   Contains Level 0 Part: 5' Connector (5C1CSN) for the construction of Level 1 plasmids

   Depositor

   Georg Fritz, Thorsten Mascher

   Article

   Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

   Insert

   5' Connector (5C1CSN)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPBT-peRNA_GG-Puro

   Plasmid

   #173220

   Purpose

   piggyBac transposon containing hU6 promotor and pegRNA cloning site

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Wolff et al Front Genome Ed. 2021 Nov 12;3:786893. d

   Inserts

   pegRNA cloning cassette
   PuroR
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-a and hU6

   Available since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKB01-PssbB-mKate2

   Plasmid

   #69105

   Purpose

   Expresses mKate2 (codon adapted for Streptococcus pneumoniae); under control of CSP inducible promoter PssbB. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.

   Depositor

   Jan-Willem Veening

   Article

   Beilharz et al Appl Environ Microbiol. 2015 Aug 7. pii:

   Insert

   mKate2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. AV09_pDonor.EP.S1.TS

   Plasmid

   #100297

   Purpose

   AAVS1-targeting expression plasmid

   Depositor

   Manuel Goncalves

   Article

   Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

   Insert

   PuroR.T2A.EGFP expression unit
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLuciferase (GB0096)

   Plasmid

   #68201

   Purpose

   Provides the CDS of the firefly luciferase protein as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   Luciferase coding region
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. AY42_pS.Donor.R5

   Plasmid

   #100291

   Purpose

   CCR5-targeting plasmid

   Depositor

   Manuel Goncalves

   Article

   Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

   Insert

   18 bp RFLP (MluI+KspAI)
   

   Use

   CRISPR and Synthetic Biology; Human targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAF256

   Plasmid

   #105496

   Purpose

   pRPF185 derived plasmid encoding an anhydrotetracyclin inducible HupA-SmBiT and LgBiT

   Depositor

   Wiep Klaas Smits

   Article

   Oliveira Paiva et al J Mol Biol. 2019 Jan 8. pii: S0022-2836(

   Insert

   HupA-SmBiT/LgBiT
   

   Use

   Atc-dependent expression in c. difficile

   Tags

   Expression

   Mutation

   Promoter

   Ptet

   Available since

   Sept. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRenilla (GB0056)

   Plasmid

   #68198

   Purpose

   Provides the CDS of the Renilla protein as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   Renilla coding region
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAF257

    Plasmid

    #105497

    Purpose

    pRPF185 derived plasmid encoding an anhydrotetracyclin inducible SmBiT and HupA-LgBiT

    Depositor

    Wiep Klaas Smits

    Article

    Oliveira Paiva et al J Mol Biol. 2019 Jan 8. pii: S0022-2836(

    Insert

    SmBiT/HupA-LgBiT
    

    Use

    Atc-dependent expression in c. difficile

    Tags

    Expression

    Mutation

    Promoter

    Ptet

    Available since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. FFiBlast MSCV Puro

    Plasmid

    #68464

    Purpose

    Retroviral plasmid for establishment of blasticidin resistance Cre-reporter cell line

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Blasticidin Resistance
    

    Use

    Cre/Lox and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Inverted

    Promoter

    LTR

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBstSK+IRES-V5-IPpoI

    Plasmid

    #206321

    Purpose

    A plasmid that facilitates production of mRNA encoding V5 epitope tagged I-PpoI

    Depositor

    Brian McStay

    Article

    van Sluis et al Genes Dev. 2015 Jun 1;29(11):1151-63. do

    Insert

    IRES element-V5 epitope tag- IPpoI open reading frame
    

    Use

    Mrna production

    Tags

    V5-IPpoI fusion protein

    Expression

    Mutation

    Promoter

    Available since

    Aug. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFA0055

    Plasmid

    #131774

    Purpose

    Guide RNA (gCASS5a) and Cas9 expression plasmid for cleaving pFA6 series deletion cassettes, including KanMX, hphMX and natMX. Used to perform CRISPR-Swap of alleles.

    Depositor

    Frank Albert

    Article

    Lutz et al PLoS Genet. 2019 Nov 18;15(11):e1008375.

    Insert

    20mer CASS5a guide (gCASS5a) and 5' sgRNA
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. AY10_pS.Donor.R5.TS

    Plasmid

    #100292

    Purpose

    CCR5-targeting plasmid

    Depositor

    Manuel Goncalves

    Article

    Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

    Insert

    18 bp RFLP (MluI+KspAI)
    

    Use

    CRISPR and Synthetic Biology; Human targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pIK198

    Plasmid

    #65629

    Purpose

    "flipped and extended" sgRNA backbone (Chen et al., 2013) following a C. elegans U6 promoter (Friedland et al., 2013). For Gibson cloning locus-specific sgRNA sequences.

    Depositor

    Iskra Katic

    Article

    Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFP122

    Plasmid

    #21669

    Depositor

    James Haber

    Article

    Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

    Insert

    Lacz (lacZ E. coli)
    

    Use

    Centromeric plasmid

    Tags

    Expression

    Mutation

    The second lacZ sequence has been modified, the E…

    Promoter

    Available since

    Sept. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

17. pAAV_CK8e_Cas9_miniPA

    Plasmid

    #226141

    Purpose

    AAV construct for HITI insert with CK8e promoter and SpCas9

    Depositor

    Christopher Nelson

    Article

    Padmaswari et al Mol Ther Nucleic Acids. 2024 Sep 2;35(4)

    Insert

    Cas9
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CK8e

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX459_LIG4_Exon3

    Plasmid

    #225363

    Purpose

    pX459 plasmid encoding the sgRNA protospacer sequence “5’-GCATAATGTCACTACAGATC-3’ to target the LIG4 gene.

    Depositor

    Sujatha Jagannathan

    Article

    Cortazar et al Methods Mol Biol. 2025;2863:397-417. doi

    Insert

    LIG4 (LIG4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. POC1550

    Plasmid

    #226497

    Purpose

    pMOBC360_VM_C1 assembled plasmid (Chloramphenicol R) containing four inserts (POC1546-POC1549) assembled in POC1525 using the site-selective methylation protection approach

    Depositor

    Christopher A. O'Callaghan

    Article

    Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

    Insert

    Assembled construct flanked by methylation-protectable BsaI sites and containing several always-methylable internal BsaI sites
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. POC1546

    Plasmid

    #226487

    Purpose

    pMOBK360_VL_N1 assembled plasmid (Kanamycin R) containing four inserts (POC1535-POC1538) assembled in POC1518 using the site-selective methylation protection approach

    Depositor

    Christopher A. O'Callaghan

    Article

    Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

    Insert

    ssembled construct flanked by methylation-protectable BsaI sites and containing several always-methylable internal BsaI sites
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge