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pLV_PGK_LTB4R

Plasmid

#208087

Purpose

Mammalian expression of the wild-type human LTB4 receptor LTB4R.

Depositor

Sean R Collins

Article

Lundgren et al Sci Signal. 2023 Oct 3;16(805):eadd1845.

Insert

LTB4R (LTB4R Human)

Use

Lentiviral

Expression

Mammalian

Mutation

none

Promoter

PGK

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pTU2-A-RFP (p15A origin)

Plasmid

#74090

Purpose

pTU2-A backbone, with RFP negative selection and p15A (low-copy) origin of replication

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Golden Gate destination vector - Level 2, 4 TU's with RFP and p15A origin

Use

Synthetic Biology

Expression

Bacterial

Promoter

R0010 Const. Promoter controlling RFP

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pPU-huTRIMmiR30-TRIM5

Plasmid

#132938

Purpose

All-in-one huTRIM5 rescue-TRIM5 shRNA knockdown

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

human TRIM5

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

SFFV

Available Since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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SEH1L in pRK5

Plasmid

#154216

Purpose

Untagged SEH1L cDNA expression vector for transfections

Depositor

David Sabatini

Article

Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

Insert

SEH1L (SEH1L Human)

Tags

NONE

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-MOV10-ts1

Plasmid

#174277

Purpose

MOV10 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

MOV10 shRNA (MOV10 Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-MOV10-ts2

Plasmid

#174278

Purpose

MOV10 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

MOV10 shRNA (MOV10 Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3 2xFLAG-2xSTREP TFDP2

Plasmid

#236445

Purpose

transient overexpression of TFDP2 in mammalian cells

Depositor

Michele Pagano

Article

Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

Insert

TFDP2 (TFDP2 Human)

Tags

FLAG-FLAG-STREP-STREP

Expression

Mammalian

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pET-NEMO

Plasmid

#11966

Depositor

Jon Ashwell

Article

Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

Insert

NEMO (IKBKG Human)

Tags

His

Expression

Bacterial

Available Since

July 13, 2006

Availability

Academic Institutions and Nonprofits only
pCfB2798

Plasmid

#63659

Purpose

pCfB2798 is a vector for multiple integrations at sites sharing homology with Ty4Cons. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Insert

GFP

Use

Cre/Lox

Expression

Yeast

Promoter

TEF1 promoter

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB2794

Plasmid

#63651

Purpose

pCfB2794 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Insert

GFP

Use

Cre/Lox

Expression

Yeast

Promoter

TEF1 promoter

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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KTK_002

Plasmid

#180527

Purpose

Entry vector containing J23104 promoter

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

J23104 promoter

Expression

Bacterial

Available Since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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pBS NL4-3 envFS eGFP Δrev

Plasmid

#101348

Purpose

All mutations in rev are silent with respect to the tat reading frame. Start ATG->ACG and nts 68-71 were mutated AGC->TCA to change tyrosine to a stop.

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3

Use

Lentiviral

Mutation

delta rev

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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Ppst93-ANME2bHR1 MCR-NG

Plasmid

#192774

Purpose

Expresses NG-tagged ANME2bHR1 MCR in Methanococcus maripaludis

Depositor

William B. Whitman

Article

Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

Insert

Methyl coenzyme M reductase

Use

Synthetic Biology; Heterologous expression in met…

Tags

Flag-Strep2

Available Since

Dec. 7, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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Ppst93-ANME1G37 MCR-NG

Plasmid

#192773

Purpose

Expresses NG-tagged ANME1G37 MCR in Methanococcus maripaludis

Depositor

William B. Whitman

Article

Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

Insert

Methyl coenzyme M reductase

Use

Synthetic Biology; Heterologous expression in met…

Tags

Flag-Strep2

Available Since

Dec. 7, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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CMV-H3.3-YFP

Plasmid

#8693

Depositor

Kami Ahmad

Article

Janicki et al Cell 2004 Mar 5;116(5):683-98.

Insert

H3.3A (His3.3A Fly)

Tags

YFP

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pCMV-Myc-XPLN

Plasmid

#73365

Purpose

Expresses myc tagged XPLN in mammalian cells

Depositor

Jie Chen, Krister Wennerberg

Article

Arthur et al J Biol Chem. 2002 Nov 8;277(45):42964-72

Insert

arhgef3 (ARHGEF3 Human)

Tags

Myc (QKLISEEDL)

Expression

Mammalian

Promoter

CMV

Available Since

April 21, 2016

Availability

Academic Institutions and Nonprofits only
pEGFP-N1-FNIP1

Plasmid

#49175

Purpose

Mammalian expression of mouse folliculin interacting protein 1 (FNIP1) fused to GFP

Depositor

Shawn Ferguson

Article

Petit et al J Cell Biol. 2013 Sep 30;202(7):1107-112

Insert

Folliculin Interacting Protein 1 (Fnip1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

Stop codon deleted

Promoter

CMV

Available Since

Dec. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pSC101 NL4-3 envFS Δrev ΔRRE RTEm26CTE

Plasmid

#101351

Purpose

HIV-1 delta Rev was cloned into pSC101 and modified to include an RTEm26CTE element in order to utilize the NXF1 RNA export pathway. The RRE was also mutated.

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-Rac1 G15A

Plasmid

#69355

Purpose

expression of nucleotide free Rac1 in bacterial cells

Depositor

Rafael Garcia-Mata

Article

Garcia-Mata et al Methods Enzymol. 2006;406:425-37.

Insert

Rac1 (RAC1 Human)

Tags

GST

Expression

Bacterial

Mutation

G15A

Promoter

tac

Available Since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only
pNC1011

Plasmid

#42898

Depositor

Beverly Errede

Article

Houser et al Yeast. 2012 Dec;29(12):519-30. doi: 10.1

Insert

3 tandem copies of GFP*

Use

Yeast integrating

Mutation

changed Phenylalanine 64 to Leucine, Serine 65 t…

Promoter

None

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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