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LHP1263 - HA-VPS9

Plasmid

#32431

Depositor

Linda Hicke

Article

Shih et al EMBO J. 2003 Mar 17;22(6):1273-81.

Insert

VPS9 (VPS9 Budding Yeast)

Tags

3xHA

Expression

Yeast

Promoter

Lac

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
7090 pNOTAG Hsp104 N728A

Plasmid

#1244

Depositor

Susan Lindquist

Article

Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

Insert

Hsp104 (N728A) (HSP104 Budding Yeast)

Expression

Bacterial

Mutation

N728A

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pU6-EF-gAAVS1

Plasmid

#112262

Purpose

Express g-AAVS1

Depositor

Zhili Rong

Article

Ma et al FEBS Lett. 2018 Jun 5. doi: 10.1002/1873

Insert

g-AAVS1

Expression

Mammalian

Promoter

U6

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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7092 pNOTAG Hsp104 R826M

Plasmid

#1243

Depositor

Susan Lindquist

Article

Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

Insert

Hsp104 (R826M) (HSP104 Budding Yeast)

Expression

Bacterial

Mutation

R826M

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
6969 pNOTAGHsp104(K620T)

Plasmid

#1237

Depositor

Susan Lindquist

Article

Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

Insert

Hsp104 (K620T) (HSP104 Budding Yeast)

Expression

Bacterial

Mutation

K620T

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
7852 pUG72-HSC82out

Plasmid

#10895

Depositor

Susan Lindquist

Article

Cowen et al Science. 2005 Sep 30. 309(5744):2185-9.

Insert

HSC82 (HSC82 Budding Yeast)

Use

Cre/Lox

Expression

Yeast

Available Since

April 26, 2006

Availability

Academic Institutions and Nonprofits only

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K218Tc-3

Plasmid

#1300

Depositor

Susan Lindquist

Article

Schirmer et al Proc Natl Acad Sci U S A 2001 Jan 30;98(

Insert

mutant HSP104 (HSP104 Budding Yeast)

Expression

Yeast

Mutation

K218T: A315T

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pKAT6

Plasmid

#1215

Depositor

Susan Lindquist

Article

Nathan et al Mol Cell Biol 1995 Jul;15(7):3917-25.

Insert

Hsc82 (HSC82 Budding Yeast)

Use

2 micron

Expression

Yeast

Mutation

vector backbone is YEp24 URA3 selection

Available Since

July 18, 2005

Availability

Academic Institutions and Nonprofits only
pYO1877

Plasmid

#235723

Purpose

Expression of FL yeast VPS15 S622D_H623D_T626E mutant-3x FLAG under its own promoter in yeast

Depositor

Roger Williams

Article

Špokaitė et al eLife

Insert

VPS15

Tags

3xFLAG

Expression

Yeast

Mutation

S622D, H623D, T626E

Promoter

ScVPS15 promoter

Available Since

March 18, 2026

Availability

Academic Institutions and Nonprofits only
pSC6

Plasmid

#233020

Purpose

Inducible Conjugative Plasmid

Depositor

Bogumil Karas

Article

Jaafar et al Can J Microbiol. 2025 May 6;71:1-9. doi:

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pGRNA2II_LacZT1

Plasmid

#247079

Purpose

L-arabinose inducible crRNA of type I-E CRISPR cas (Escherichia coli K-12). encoding 105 nt spacer which targets Ecoli LacZ T1 site

Depositor

Seokhee Kim

Article

Lee et al Nat Commun. 2025 Nov 25. doi: 10.1038/s4

Insert

type I-E CRISPR cas crRNA

Use

CRISPR

Expression

Bacterial

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pGRNA1_105nt

Plasmid

#247075

Purpose

L-arabinose inducible crRNA of type I-E CRISPR cas (Escherichia coli K-12). encoding 105 nt spacer which targets pheS*_codon

Depositor

Seokhee Kim

Article

Lee et al Nat Commun. 2025 Nov 25. doi: 10.1038/s4

Insert

type I-E CRISPR cas crRNA

Use

CRISPR

Expression

Bacterial

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pMut3_pm32B

Plasmid

#247071

Purpose

L-arabinose inducible pmCDA-XTEN32-CasB(Cas11)-GSSG-UGI

Depositor

Seokhee Kim

Article

Lee et al Nat Commun. 2025 Nov 25. doi: 10.1038/s4

Insert

PmCDA-XTEN32-Cas11(CasB)-UGI

Use

CRISPR

Expression

Bacterial

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pGRNA2I_LacZB1

Plasmid

#247076

Purpose

L-arabinose inducible crRNA of type I-E CRISPR cas (Escherichia coli K-12). encoding 105 nt spacer which targets Ecoli LacZ B1 site

Depositor

Seokhee Kim

Article

Lee et al Nat Commun. 2025 Nov 25. doi: 10.1038/s4

Insert

type I-E CRISPR cas crRNA

Use

CRISPR

Expression

Bacterial

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pGRNA3_LacZ_B1_IIB5

Plasmid

#247081

Purpose

L-arabinose inducible crRNA of type I-E CRISPR cas (Escherichia coli K-12). encoding 105 nt spacer which targets both Ecoli LacZ B1 and B5 site

Depositor

Seokhee Kim

Article

Lee et al Nat Commun. 2025 Nov 25. doi: 10.1038/s4

Insert

type I-E CRISPR cas crRNA

Use

CRISPR

Expression

Bacterial

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pBID2_Gateway>QFG4

Plasmid

#173729

Purpose

Drosophila QF:Gal4 expression under introduced sequence control, PhiC31 transgenesis

Depositor

Brian McCabe

Article

Ruchti et al bioRxiv 2026.02.02.703225

Type

Empty backbone

Use

Gateway destination vector

Expression

Insect

Mutation

QF DNA binding domain, GAL4 activation domain

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pBID2_Gateway>LexQF

Plasmid

#173727

Purpose

Drosophila LexA:QF expression under introduced sequence control, PhiC31 transgenesis

Depositor

Brian McCabe

Article

Ruchti et al bioRxiv 2026.02.02.703225

Type

Empty backbone

Use

Gateway destination vector

Expression

Insect

Mutation

LexA DNA binding domain, QF activation domain

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1880

Plasmid

#235721

Purpose

Expression of FL yeast VPS15 S622D_H623D_T626E mutant -EGFP under its own promoter in yeast

Depositor

Roger Williams

Article

Špokaitė et al eLife

Insert

VPS15

Expression

Yeast

Mutation

S622D, H623D, T626E

Promoter

ScVPS15 promoter

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only
pgfaABC1D-mCherry-CAAX

Plasmid

#196488

Purpose

To over express mCherry-CAAX under gfaABC1D promoter in astrocytes

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Insert

mCherry-CAAX

Use

AAV

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGEARBOCS-v0-Gfap-KO

Plasmid

#196489

Purpose

To KO Gfap in mouse astrocytes

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Insert

gRNA

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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