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CBS_pCSdest

Plasmid

#53909

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

CBS (CBS Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
pExT

Plasmid

#36889

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

T (i.e., ATG, start codon as a first exon)
GFP
beta-globin intron
Hygro

Expression

Mammalian

Mutation

deleted nucleotides before nucleotide 275 and ins…

Promoter

CAG (chicken beta actin promoter and CMV enhancer…

Available Since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pExG

Plasmid

#36888

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP
tdTomato-3Myc
beta-globin intron
Hygro

Tags

3 Myc tags

Expression

Mammalian

Mutation

deleted nucleotides after nucleotide 274, inserti…

Promoter

CAG (chicken beta actin promoter and CMV enhancer…

Available Since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pLib6.4-Cquin_596

Plasmid

#176655

Purpose

Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039596) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pSL1180-HR-PUbEGFP-NLS

Plasmid

#176681

Purpose

Expression of nuclear localized EGFP under Ae. aegypti Polyubiquitin promoter (AAEL003888), EGFP version of pSL1180-HR-PUbECFP (Addgene #47917)

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Insert

EGFP

Expression

Insect

Promoter

Ae. aegypti poly-ubiquitin (AAEL003888)

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ2300 - [1-2] ENTR - Fluor - ce-Dendra2(1 intron, syntrons(3), no_atg, no_stop)

Plasmid

#159869

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-Dendra2(1 intron, syntrons(3), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV DCC mut

Plasmid

#16460

Depositor

Bert Vogelstein

Article

Fearon et al Science. 1990 Jan 5. 247(4938):49-56.

Insert

DCC (DCC Human)

Expression

Mammalian

Mutation

5' end of DCC

Available Since

March 10, 2008

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-H2-Ld

Plasmid

#135519

Purpose

Mammalian expression of myc-tagged H2-Ld

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

H2-Ld

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pMtb CblA

Plasmid

#138298

Purpose

Expression of Mtb CblA

Depositor

Ruma Banerjee

Article

Ruetz et al Science. 2019 Nov 1;366(6465):589-593. d

Insert

Mtb CblA

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS-synPCB2.0

Plasmid

#139477

Purpose

Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

Depositor

Kazuhiro Aoki

Article

Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

Insert

MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Fnr-Fd-T7

Expression

Mammalian

Mutation

V413L

Promoter

CAG promoter

Available Since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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pBSK-SN3 (wt p53)

Plasmid

#16421

Depositor

Bert Vogelstein

Article

Baker et al Science. 1989 Apr 14. 244(4901):217-21.

Insert

p53 (TP53 Human)

Expression

Bacterial

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
sg2.02 MUC4.1

Plasmid

#101152

Purpose

gRNA with two MCP binding sites

Depositor

Mazhar Adli

Article

Qin et al Nat Commun. 2017 Mar 14;8:14725. doi: 10

Insert

MUC4 (MUC4 Human)

Use

CRISPR and Lentiviral

Promoter

mU6

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV4-Flag-mIL-17RA/YFP.Hygro

Plasmid

#46862

Purpose

encodes Flag-tagged mouse IL-17RA fused to Yellow Fl. Protein

Depositor

Sarah Gaffen

Article

Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

IL-17RA-YFP

Tags

Flag/IL-3 signal sequence and fused to CFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only
IGSF5_pCSdest

Plasmid

#53886

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

IGSF5 (IGSF5 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
pENTR4_FLAG/HA_FUS_His6

Plasmid

#26365

Depositor

Thomas Tuschl

Article

Spitzer et al Genes Chromosomes Cancer. 2011 May . 50(

Insert

FUS (FUS Human)

Use

Entry vector

Tags

FLAG/HA and His6

Mutation

delta 527 (stop codon)

Available Since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only
pCFJ2240 - [1-2] ENTR - Fluor - ce-tagRFP-T (1 intron, syntrons(3), no_atg, no_stop)

Plasmid

#159855

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-tagRFP-T (1 intron, syntrons(3), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMRE151

Plasmid

#118501

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mTurquoise2

Expression

Bacterial

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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MEIS1A-pSP65

Plasmid

#21014

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 1997 Nov;17(11):6448-58

Insert

MEIS1A (Meis1 Mouse)

Use

In vitro tnt

Tags

Flag

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
vsv-CenpB 1-166-Sgo1-GFP

Plasmid

#108495

Purpose

expression of vsv-CenpB-1-166-Sgo1-EGFP

Depositor

Susanne Lens

Article

Meppelink et al Cell Rep. 2015 Apr 28;11(4):508-15. doi:

Insert

CenpB-Shugoshin 1 (SGO1 Human)

Tags

EGFP and VSV

Expression

Mammalian

Mutation

CenpB 1-166 fusion before Sgo1

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pIhh742bp-Luc

Plasmid

#53605

Purpose

Reporter construct containing a 742 bp promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assays

Depositor

Xiangli Yang

Article

Wang et al Development. 2009 Dec;136(24):4143-53. d

Insert

Ihh promoter

Use

Luciferase; Reporter

Available Since

June 30, 2014

Availability

Academic Institutions and Nonprofits only