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We narrowed to 5,900 results for: pcas

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Showing: 2521 - 2540 of 5900 results

  2. pFS_0272_pET30_pCat-tetR-Term-ptetA-Rluc-rrnBterm_Fusicatenibacter_saccharivorans_ARRAY2_FaqI

    Plasmid
    #116956
    Purpose
    Expresses FsRT-Cas1-Cas2 under pT7lac and Rluc under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout
    Depositor
    Randall Platt
    Article
    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:
    Insert
    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    116956-plasmid-map-sequence-id-227069

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  7. SIN-cPPT-G1B3-spCas9-WPRE-miR124T

    Plasmid
    #245071
    Purpose
    CRISPR Editing with SpCas9, with miRNA-124 target sequence to repress transgene expression in neurons
    Depositor
    Nicole Déglon
    Insert
    Cas9, miR124T site
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    Human G1B3 promoter
    Available Since
    Dec. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    245071-plasmid-map-sequence-id-495712

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  9. pCMV-SpCas9-VQR-P2A-EGFP (LRF199)

    Plasmid
    #242664
    Purpose
    CMV promoter expression plasmid for human codon optimized SpCas9-VQR-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-SpCas9-VQR-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    VQR mutations in SpCas9(D1135V/R1335Q/T1337R)
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242664-plasmid-map-sequence-id-488971

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  19. pCMV-T7-SpCas9(SRQMRG)-P2A-EGFP (RAS3017)

    Plasmid
    #223103
    Purpose
    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with SRQMRG amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(SRQMRG)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(SRQMRG)=D1135S, S1136R, G1218Q, E1219M, R1…
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223103-plasmid-map-sequence-id-447689

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  20. pCMV-T7-SpCas9(MRKQKC)-P2A-EGFP (RAS2838)

    Plasmid
    #223104
    Purpose
    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MRKQKC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(MRKQKC)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(MRKQKC)=D1135M, S1136R, G1218K, E1219Q, R1…
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223104-plasmid-map-sequence-id-447670

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Showing: 2521 - 2540 of 5900 results