We narrowed to 8,708 results for: sgRNA

1. pPSG804

   Plasmid

   #239534

   Purpose

   CRISPR plasmid with Cas9 and sgRNA targeting YDR514C locus in S. cerevisiae

   Depositor

   John Dueber

   Insert

   sgRNA_YDR514C
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPSG779

   Plasmid

   #239532

   Purpose

   CRISPR plasmid withCas9 and sgRNA targeting YBR197C locus in S. cerevisiae

   Depositor

   John Dueber

   Insert

   sgRNA_YBR197C
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPSG803

   Plasmid

   #239533

   Purpose

   CRISPR plasmid with Cas9 and sgRNA targeting YMR206W locus in S. cerevisiae

   Depositor

   John Dueber

   Insert

   sgRNA_YMR206W
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LHZ1493

   Plasmid

   #222102

   Purpose

   Cloning backbone for sgRNA in Kluyveromyces marxianus. Expresses highly specific SlugCas9.

   Depositor

   Yongming Wang

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. LHZ1494

   Plasmid

   #222104

   Purpose

   Triple sgRNAs expression plasmid in Kluyveromyces marxianus.

   Depositor

   Yongming Wang

   Insert

   sg1RNA, sg4RNA, sg7RNA
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pX330-PITCh-TOMM20

   Plasmid

   #207789

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of TOMM20 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of TOMM20 (TOMM20 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330-PITCh-VIM

   Plasmid

   #227301

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of VIM for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of VIM (VIM Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330-PITCh-MAPRE1

   Plasmid

   #207793

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of MAPRE1 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of MAPRE1 (MAPRE1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pX330-CEP135

   Plasmid

   #227286

   Purpose

   Expresses SpCas9 and a sgRNA targeting the N-terminus of CEP135 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting N-terminus of CEP135 (CEP135 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pL-CRISPR.SFFV.GFP

    Plasmid

    #57827

    Purpose

    Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. SFFV Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    SpCas9
    Sp sgRNA scaffold
    SFFV
    P2A-eGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330-PITCh-GOLGA2

    Plasmid

    #207791

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of GOLGA2 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting N-terminus of GOLGA2 (GOLGA2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pX330-PITCh-LMNB1

    Plasmid

    #207770

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of LMNB1 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting N-terminus of LMNB1 (LMNB1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. lentiSAM v2 (Puro)

    Plasmid

    #92062

    Purpose

    Modified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Adam Karpf

    Article

    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (sgRNA) and EF1a (dCas9-VP64)

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO-CreERT2 stuffer v3

    Plasmid

    #158046

    Purpose

    lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA casette (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9

    Depositor

    Daniel Schramek

    Article

    Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. BPK764

    Plasmid

    #65767

    Purpose

    Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7 (x2)

    Available since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC57-Amp_CRISPIE donor B5

    Plasmid

    #172846

    Purpose

    CRISPIE donor B5 (Zhong et al, eLife 2021), CDS of VCL exon21-mEGFP, translational phase (0-stop), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    CRISPIE designer exon (phase 0-stop) encoding the CDS of VCL exon 21 fused to mEGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLKO-Cre stuffer v4

    Plasmid

    #158032

    Purpose

    lenti-viral construct with Cre recombinase and U6 driven sgRNA casette with modified tracrRNA (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9

    Depositor

    Daniel Schramek

    Article

    Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pCRISPR-Cre stuffer v4

    Plasmid

    #158033

    Purpose

    Lentiviral construct with Cas9, Cre recombinase and U6 driven sgRNA cassette with modified tracr RNA (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI)

    Depositor

    Daniel Schramek

    Article

    Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MSP1673

    Plasmid

    #65769

    Purpose

    Bacterial expression plasmid for S. thermophilus1 Cas9 & sgRNA (need to clone in spacer into BspMI sites): T7-humanSt1Cas9-NLS-T7-BspMIcassette-St1-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7 (x2)

    Available since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pX330-LAMP1

    Plasmid

    #207787

    Purpose

    Expresses SpCas9 and a sgRNA targeting the C-terminus of LAMP1 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of LAMP1 (LAMP1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge