We narrowed to 16,228 results for: sgRNA

1. PGL3-U6-ALDOB_pegRNA-Csy4RS-nick_sgRNA-EGFP

   Plasmid

   #172668

   Purpose

   For expression of transcript containing pegRNA and nick-sgRNA targeting human ALDOB gene from the U6 promoter with pegRNA flanked by Csy4 recognition site

   Depositor

   Xiaolong Wang

   Article

   Liu et al Cell Res. 2021 Jun 8. pii: 10.1038/s4142

   Insert

   ALDOB pegRNA and ALDOB_nick-sgRNA
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_gcrA1

   Plasmid

   #133340

   Purpose

   for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets gcrA gene's promoter on the non-template strand in Caulobacter crescentus

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   sgRNA_gcrA
   

   Use

   CRISPR

   Promoter

   constitutive

   Available Since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHBS953 TARDBP-Exon2-ko-sgRNA2-SpCas9

   Plasmid

   #107858

   Purpose

   To knock-out TDP43

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   gRNA against TARDBP (Exon 2) (TARDBP Human)
   

   Expression

   Mammalian

   Available Since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBXMCS-2 Pconstitutive-sgRNA(Spy)_ctrA

   Plasmid

   #133334

   Purpose

   for constitutive expression of a single guide RNA from Streptococcus pyogenes with a seed region that targets ctrA gene's promoter from Caulobacter crescentus

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   sgRNA_ctrA
   

   Use

   CRISPR

   Promoter

   constitutive

   Available Since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC19-U6-BsmBI_cassette-CjeCas9-sgRNA (KAC482)

   Plasmid

   #133793

   Purpose

   U6 promoter sgRNA entry vector used for all CjeCas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to V2-TGAA linker sgRNA architecture from Kim et al. Nature Communications 2017

   Depositor

   Benjamin Kleinstiver, Joseph Bondy-Denomy

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   CjeCas9 sgRNA entry vector
   

   Expression

   Mammalian

   Mutation

   V2-TGAA linker sgRNA architecture from Kim et al.…

   Promoter

   U6

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pdCas9-sgRNA-com-BoxB-vector

   Plasmid

   #132556

   Purpose

   Expresses dCas9-DMNT3a-DMNT3l fusion protein, lambda N22-KRAB fusion protein, and sgRNA scaffold with com and BoxB replacing the Tetraloop and the loop 2 respectively.

   Depositor

   Baisong Lu

   Inserts

   dCas9-DMNT3a-DMNT3l (DNMT3A Synthetic)
   Lambda N22-KRAB
   sgRNA acaffold with com and BoxB replacing the Tetraloop and the loop 2.
   

   Expression

   Mammalian

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pspCas9-ABE-3'UTR-sgRNA-2xMS2

   Plasmid

   #132554

   Purpose

   Vector plasmid expressing ABEmax and sgRNA scaffold with MS2 replacing the Tetraloop and loop 2

   Depositor

   Baisong Lu

   Article

   Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1

   Inserts

   ABEmax
   sgRNA, with Tetraloop and loop 2 replaced by MS2 aptamer
   

   Expression

   Mammalian

   Available Since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. PGL3-U6-RNF2_pegRNA-Csy4RS-nick_sgRNA-EGFP

   Plasmid

   #172669

   Purpose

   For expression of transcript containing pegRNA and nick-sgRNA targeting human RNF2 gene from the U6 promoter with pegRNA flanked by Csy4 recognition site

   Depositor

   Xiaolong Wang

   Article

   Liu et al Cell Res. 2021 Jun 8. pii: 10.1038/s4142

   Insert

   RNF2 pegRNA and RNF2_nick-sgRNA
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMuLE ENTR U6 stuffer sgRNA scaffold L1-R5

   Plasmid

   #62127

   Purpose

   MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and sgRNA scaffold module for gRNA expression. Compatible with MultiSite Gateway cloning

   Depositor

   Ian Frew

   Article

   Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

   Type

   Empty backbone

   Use

   CRISPR; Mule gateway entry vector

   Expression

   Mammalian

   Available Since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. lentiCRISPR - Amplicon, BCR/ABL sgRNA 1

    Plasmid

    #70659

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic BCR/ABL-targeting sgRNA element from U6 promoter. Lentiviral backbone

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    sgRNA against BCR/ABLamplicon found in CML-derived K562 cell line
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. 8xCTS1_WT-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_WT-hutRNApro_SV40polyA

    Plasmid

    #124541

    Purpose

    Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    WT-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_WT-hutRNApro
    

    Expression

    Mammalian

    Mutation

    WT, WT

    Promoter

    Minimal ADE

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. CAGGS-AsCpf1-2A-GFP-U6-AAVS1-sgRNA

    Plasmid

    #194723

    Purpose

    based on (CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector, Addgene 159281), with guide RNA that target hAAVS1

    Depositor

    Rudolf Jaenisch

    Article

    Haiting et al bioRxiv

    Insert

    AsCpf1 (AAVS1 Acidaminococcus sp.)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMuLE ENTR U6 stuffer sgRNA scaffold L5-L4

    Plasmid

    #62129

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and sgRNA scaffold module for gRNA expression. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Type

    Empty backbone

    Use

    CRISPR; Mule gateway entry vector

    Expression

    Mammalian

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 8xCTS1_deltaC55A-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

    Plasmid

    #124539

    Purpose

    Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    deltaC55A-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro
    

    Expression

    Mammalian

    Mutation

    d11-41 C55A, d11-41

    Promoter

    Minimal ADE

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. Px458-Cas9n-Trp63-Exon4-2sgRNA

    Plasmid

    #88849

    Purpose

    CRISPR KO of Trp63

    Depositor

    Joan Massague

    Article

    Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

    Insert

    Trp63 (Trp63 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

16. pCF824_U6-Sau-sgRNA-EF1a-mCherry2_lenti

    Plasmid

    #138318

    Purpose

    Lenti expression of mCherry2 with U6 promoter for SauCas9 sgRNA targeting GFP

    Depositor

    Jennifer Doudna

    Article

    Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

    Insert

    SauCas9 GFP sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti pTERT sgRNA2(MS2) mCherry

    Plasmid

    #171055

    Purpose

    Targeting of Vp64-dCas9 activation system to hTERT promoter

    Depositor

    Agnel Sfeir

    Article

    Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

    Insert

    pTERT sgRNA2(MS2) mCherry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330-Flag-wtSpCas9 (without sgRNA)

    Plasmid

    #92353

    Purpose

    Expression plasmid for human codon-optimized wild-type SpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    3xFlag-NLS-wild-type SpCas9-NLS
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMuLE ENTR U6 stuffer sgRNA scaffold L5-L2

    Plasmid

    #62130

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and sgRNA scaffold module for gRNA expression. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Type

    Empty backbone

    Use

    CRISPR; Mule gateway entry vector

    Expression

    Mammalian

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_cpaA

    Plasmid

    #133344

    Purpose

    for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets cpaA gene's promoter from Caulobacter crescentus

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    sgRNA_cpaA
    

    Use

    CRISPR

    Promoter

    constitutive

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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