We narrowed to 22,151 results for: tes

1. pCMV-IRES-Renilla Luciferase-IRES-Gateway-Firefly Luciferase (pIRIGF)

   Plasmid

   #101139

   Purpose

   Luciferase reporter plasmid. Note that both renilla luciferase and ORF-firefly luciferase are encoded by a single mRNA and both are translated independently via internal ribosomal binding sites.

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmTurquoise2-LAMP1

   Plasmid

   #110948

   Purpose

   Expresses LAMP1 fused to mTurquoise2

   Depositor

   Pablo Rivera-Fuentes

   Article

   Halabi et al Nat Commun. 2019 Mar 15;10(1):1232. doi:

   Insert

   Lysosomal-associated membrane protein 1 (LAMP1 Human)
   

   Use

   Tags

   mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-sgRNA(lib)-puro

   Plasmid

   #119976

   Purpose

   Lenti sgRNA cloning backbone with CMV-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Wensheng Wei

   Article

   Liu et al Nat Biotechnol. 2018 Nov 5. pii: nbt.428

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA-Flag-H3.3-polyA

   Plasmid

   #59780

   Purpose

   Express Flag-tagged H3.3 (H3F3B) in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   H3F3B (H3-3B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Sept. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. Calnexin-HT8-mGold

   Plasmid

   #183989

   Purpose

   Expresses fluorescent HaloTag8 fusion protein in mammalian cells with localization in the ER.

   Depositor

   Pablo Rivera-Fuentes

   Article

   Emmert et al Nat Chem. 2023 Oct;15(10):1415-1421. doi

   Insert

   Calnexin (CANX Human)
   

   Use

   Tags

   HaloTag8 (C61S, C262S), mGold

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRISPR-cBEST

   Plasmid

   #125689

   Purpose

   C to T base editor for actinomycetes

   Depositor

   Tilmann Weber

   Article

   Tong et al Proc Natl Acad Sci U S A. 2019 Oct 8;116

   Insert

   streptomyces codon optimized spCas9n (D10A), modified sgRNA casette, streptomyces codon optimized rAPOBEC1
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   ermE*/tipA

   Available since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPLN-hFluc-WT-HA-GFP11-KDEL

   Plasmid

   #177722

   Purpose

   Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminus

   Depositor

   Erik Böttger

   Article

   Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

   Insert

   hFluc
   

   Use

   Tags

   GFP11, HA, KDEL, and Prolactin Signal Sequence

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. HA-MBP-TEVpCS-ADGRA3 CTFdeltaTA-FLAG

   Plasmid

   #217713

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRA3 (ADGRA3 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-743

   Promoter

   Available since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. HA-MBP-TEVpCS-ADGRA2 CTFdeltaTA-FLAG

   Plasmid

   #217712

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRA2 (ADGRA2 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-752

   Promoter

   Available since

   April 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AgDD

    Plasmid

    #78289

    Purpose

    create protein aggregates in living cells

    Depositor

    Thomas Wandless

    Article

    Miyazaki et al Nat Commun. 2016 May 27;7:11689. doi: 10

    Insert

    NES-L106P-sfGFP (FKBP1A Human)
    

    Use

    Tags

    MLALKLAGLDI

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pATZ-Myc

    Plasmid

    #177718

    Purpose

    ER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant), myc tagged

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    alpha-1-antitrypsin (SERPINA1 Human)
    

    Use

    Tags

    Myc

    Expression

    Bacterial and Mammalian

    Mutation

    E342K (ATZ mutant)

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p403 – TDH3-MTS-mCherry-GFP1-10-His

    Plasmid

    #177730

    Purpose

    mCherry and the 1-10th β-strands of GFP (GFP1-10) fused to a mitochondria signal sequence for mitochondrial targeting in yeast plasmid with HIS marker

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    mCherry-GFP1-10
    

    Use

    Tags

    Mitochondrial Targeting Sequence

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pmTurquoise2-LC3

    Plasmid

    #110947

    Purpose

    Expresses the autophagosome marker LC3 fused to mTurquoise2

    Depositor

    Pablo Rivera-Fuentes

    Article

    Nguyen et al Org Biomol Chem. 2021 Mar 28;19(12):2681

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. NLS-AgDD

    Plasmid

    #80625

    Purpose

    create nuclear protein aggregates in mammalian cells

    Depositor

    Thomas Wandless

    Article

    Miyazaki et al Nat Commun. 2016 May 27;7:11689. doi: 10

    Insert

    NLS-NES-L106P-sfGFP (FKBP1A Human)
    

    Use

    Tags

    MPPKKKRKVLALKLAGLDI

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pATZ-HA-GFP11

    Plasmid

    #177720

    Purpose

    ER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant) fused to an HA tag and the eleventh β-strand of GFP

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    alpha-1-antitrypsin (SERPINA1 Human)
    

    Use

    Tags

    GFP11 and HA

    Expression

    Bacterial and Mammalian

    Mutation

    E342K (ATZ mutant)

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mCherry-NES-SspB-MCS-P2A-iLIDcaax

    Plasmid

    #173869

    Purpose

    Mammalian expression of mCherry-NES-SspB-P2A-iLIDcaax (opto-control) containing multiple cloning sites (MCS)

    Depositor

    Takao Nakata

    Article

    Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

    Insert

    mCherry-NES-SspB-MCS-P2A-iLIDcaax
    

    Use

    Tags

    C-terminal CAAX-box and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDM002-sgRNA-lenti-ZeoR

    Plasmid

    #200060

    Purpose

    Lentiviral vector for expression of sgRNA with mCherry, zeocin resistance, BlpI + BstXI cloning sites

    Depositor

    Britt Glaunsinger

    Article

    McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

    Insert

    Bleomycin resistance, mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcdna3 Flag Jip1b

    Plasmid

    #52123

    Purpose

    a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

    Depositor

    Roger Davis

    Article

    Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

    Insert

    JNK interacting protein-1b (JIP-1b) (Mapk8ip1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HA-MBP-TEVpCS-ADGRG6 CTF-FLAG

    Plasmid

    #217704

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGR6 (ADGRG6 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-840

    Promoter

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HA-MBP-TEVpCS-ADGRG6 CTFdeltaTA-FLAG

    Plasmid

    #217737

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGR6 (ADGRG6 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-846

    Promoter

    Available since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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